| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
KDM5B (lysine‑specific demethylase 5B) – no IC50/Ki/EC50 values provided; AS8351 is proposed to inhibit KDM5B by competing with α‑ketoglutarate for iron chelation, based on functional knockdown studies. [2]
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| ln Vitro |
By competing with α-ketoglutarate (α-KG) to chelate iron [Fe(II)] in specific epigenetic enzymes, like the JmjC-containing structure that needs α-KG and iron domain-based histone demethylase (JmjC-KDM) as a cofactor, AS8351 influences epigenetic modifications [2].
AS8351 was included in a 9‑compound cocktail (9C: CHIR99021, A‑83‑01, BIX01294, AS8351, SC1, Y27632, OAC2, SU16F, JNJ10198409) that converted human foreskin fibroblasts (HFFs) and human fetal lung fibroblasts (HLFs) into chemically induced cardiomyocyte‑like cells (ciCMs). Removal of AS8351 from 9C significantly reduced the number of beating clusters and the percentage of cTNT⁺ cells, indicating it is indispensable for cardiac reprogramming (Fig. 1E, fig. S2B). [2] Knockdown of KDM5B by shRNA or use of the KDM5B inhibitor PBIT phenocopied the effect of AS8351 in generating ciCMs, suggesting that KDM5B is a functional target of AS8351. AS8351 is hypothesized to affect epigenetic modifications by competing with α‑ketoglutarate for chelating iron/Fe(II) in JmjC‑domain‑containing histone demethylases. [2] |
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| ln Vivo |
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| Cell Assay |
Cell reprogramming assay: Human foreskin fibroblasts (HFFs) were treated with a cocktail of 9 compounds including AS8351 at a concentration of 5 μM (table S2) for 6 days in DMEM/F12 medium supplemented with 10% FBS and 1% nonessential amino acids. Then cells were cultured in cardiac induction medium (CIM) containing activin A, BMP4, VEGF, and CHIR99021 for 5 days, followed by replating in human CM‑conditioned medium. At day 30, cells were analyzed for beating clusters, cTNT expression by flow cytometry, and cardiomyocyte markers by immunofluorescence. [2]
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| Animal Protocol |
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| References | |||
| Additional Infomation |
AS8351 was identified as an indispensable component of a 9‑compound chemical cocktail (9C) that reprograms human fibroblasts into functional cardiomyocytes (ciCMs). It is a KDM5B inhibitor, and its removal significantly reduces reprogramming efficiency. The compound is thought to modulate chromatin structure by promoting active histone methylation marks (H3K4me3, H3K27ac) at heart developmental gene loci. [2]
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| Molecular Formula |
C17H13N3O2
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|---|---|---|
| Molecular Weight |
291.3
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| Exact Mass |
291.101
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| Elemental Analysis |
C, 70.09; H, 4.50; N, 14.42; O, 10.98
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| CAS # |
796-42-9
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| Related CAS # |
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| PubChem CID |
135400486
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.364g/cm3
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| Boiling Point |
503.5ºC at 760 mmHg
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| Melting Point |
266 °C
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| Flash Point |
258.3ºC
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| Index of Refraction |
1.715
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| LogP |
3.095
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
407
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1C=CN=CC=1)NN=CC1C2C(=CC=CC=2)C=CC=1O
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| InChi Key |
WQSHHAVECQJKLX-YBFXNURJSA-N
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| InChi Code |
InChI=1S/C17H13N3O2/c21-16-6-5-12-3-1-2-4-14(12)15(16)11-19-20-17(22)13-7-9-18-10-8-13/h1-11,21H,(H,20,22)/b19-11+
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| Chemical Name |
N-[(E)-(2-hydroxynaphthalen-1-yl)methylideneamino]pyridine-4-carboxamide
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.58 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4329 mL | 17.1644 mL | 34.3289 mL | |
| 5 mM | 0.6866 mL | 3.4329 mL | 6.8658 mL | |
| 10 mM | 0.3433 mL | 1.7164 mL | 3.4329 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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