| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Arborine targets the peripheral effects of acetylcholine, acting as an inhibitor. This suggests it may interact with muscarinic acetylcholine receptors (mAChRs). By inhibiting the action of acetylcholine, it can induce a fall in blood pressure. Its mechanism of action is not fully detailed but is primarily related to its anticholinergic effects.
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| ln Vitro |
In vitro, Arborine inhibits the peripheral effects of acetylcholine. This activity is likely assessed by measuring its ability to block acetylcholine-induced contractions in isolated smooth muscle preparations or by its ability to displace radiolabeled ligands from mAChRs. It also has a growth inhibitory effect on Drosophila melanogaster larvae.
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| ln Vivo |
In vivo, Arborine induces a decrease in blood pressure. This effect is attributed to its inhibition of the peripheral actions of acetylcholine. Its potential as a hypotensive agent has been noted, but it is not used clinically.
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| Enzyme Assay |
In vitro non-cell enzyme/receptor binding assays for Arborine would typically involve radioligand binding studies to determine its affinity for muscarinic acetylcholine receptors. Membranes from cells expressing these receptors are incubated with the compound and a radiolabeled antagonist, and the Ki is determined.
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| Cell Assay |
In vitro cell-based assays for Arborine could involve measuring its effects on acetylcholine-induced signaling in cell lines expressing mAChRs. This could include measuring changes in intracellular calcium levels or other downstream signaling events. Its effects on cell proliferation could also be studied in Drosophila cell lines.
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| Animal Protocol |
In vivo animal studies for Arborine would likely involve measuring its effects on blood pressure in rodent models. The compound would be administered, and its hypotensive effect would be monitored. Its effects on other physiological parameters could also be assessed.
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| ADME/Pharmacokinetics |
Arborine has a molecular weight of 250.30 g/mol and a molecular formula of C₁₆H₁₄N₂O. It is a quinazolone alkaloid. As a small, lipophilic molecule, it is expected to be well-absorbed and to cross the blood-brain barrier. Detailed pharmacokinetic data are not widely published.
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| Toxicity/Toxicokinetics |
The toxicity profile of Arborine is not extensively detailed in the provided search results. As an alkaloid, it may have significant biological activity and potential toxicity. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Arborine is a member of the quinazoline class of compounds. It has been reported that Virginia creeper contains Arborine, and relevant data is available for reference.
Arborine is a quinazolone alkaloid from Glycosmis arborea that inhibits the peripheral effects of acetylcholine and induces hypotension. It also has insecticidal activity. It is also known as Glycosine. It is not approved for clinical use and is intended for research purposes only. |
| Molecular Formula |
C16H14N2O
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|---|---|
| Molecular Weight |
250.2952
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| Exact Mass |
250.11
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| CAS # |
6873-15-0
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| PubChem CID |
63123
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
421.0±38.0 °C at 760 mmHg
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| Melting Point |
161 - 162 °C
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| Flash Point |
208.4±26.8 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.622
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| LogP |
2.25
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
19
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| Complexity |
371
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
XVPZRKIQCKKYNE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H14N2O/c1-18-14-10-6-5-9-13(14)16(19)17-15(18)11-12-7-3-2-4-8-12/h2-10H,11H2,1H3
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| Chemical Name |
2-benzyl-1-methylquinazolin-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~66.60 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (6.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (6.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9952 mL | 19.9760 mL | 39.9521 mL | |
| 5 mM | 0.7990 mL | 3.9952 mL | 7.9904 mL | |
| 10 mM | 0.3995 mL | 1.9976 mL | 3.9952 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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