| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Antiarol targets reactive oxygen species (ROS) and free radicals, acting as a DPPH free radical scavenger. As a phenolic antioxidant, it neutralizes free radicals through hydrogen atom donation, protecting biological molecules from oxidative damage.
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|---|---|
| ln Vitro |
Antiarol exhibits moderate DPPH free radical scavenging activity, indicating its potential as an antioxidant agent. Its antioxidant capacity is attributed to the phenolic hydroxyl group and the electron-donating methoxy substituents that stabilize the resulting phenoxyl radical.
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| ln Vivo |
In vivo antioxidant activity of Antiarol has not been extensively documented. As a phenolic compound, it may be expected to exhibit systemic antioxidant effects similar to other plant-derived phenols, though specific animal model data are limited.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays for Antiarol typically involve DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assays. The compound is incubated with DPPH solution and the decrease in absorbance at 517 nm is measured to calculate the scavenging activity and IC₅₀ value.
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| Cell Assay |
In vitro cell-based assays for Antiarol typically use cell culture models of oxidative stress, where cells are treated with Antiarol followed by oxidative challenge (e.g., H₂O₂ or UV exposure). Cellular ROS levels, antioxidant enzyme activities (SOD, CAT, GPx), and cell viability are measured to evaluate cytoprotective effects.
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| Animal Protocol |
In vivo animal studies for Antiarol are limited. Standard protocols for evaluating antioxidant compounds involve administering the compound orally or intraperitoneally to rodents, followed by induction of oxidative stress (e.g., via CCl₄ or LPS), and measurement of serum/ tissue antioxidant markers, lipid peroxidation, and histopathological examination.
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| ADME/Pharmacokinetics |
Antiarol has a molecular weight of 184.19 g/mol and formula C₉H₁₂O₄. It is soluble in DMSO (150 mg/mL) and sparingly soluble in water (2 mg/mL). Melting point is 145-149°C. Its small molecular weight and moderate lipophilicity suggest potential for oral absorption, though detailed PK data are not available.
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| Toxicity/Toxicokinetics |
Antiarol is considered relatively non-toxic based on its classification as a phenolic natural product. The oral LD₅₀ is expected to be moderate to high, as with similar trimethoxyphenol compounds. Skin and eye irritation may occur with direct contact.
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| References | |
| Additional Infomation |
3,4,5-Trimethoxyphenol belongs to the phenolic class of compounds, with a structure in which phenolic compounds are substituted with methoxy groups at positions 3, 4, and 5. It is a plant metabolite. It belongs to both the phenolic and methoxybenzene classes. 3,4,5-Trimethoxyphenol has been reported to exist in persimmon (Diospyros eriantha), Tarenna attenuata, and other organisms with relevant data.
Antiarol is primarily used as a research compound for studying antioxidant mechanisms and as a reference standard in natural product chemistry. It is also known as 3,4,5-trimethoxyphenol and is used in organic synthesis and phytochemical research. Not approved for clinical use. |
| Molecular Formula |
C9H12O4
|
|---|---|
| Molecular Weight |
184.1892
|
| Exact Mass |
184.073
|
| CAS # |
642-71-7
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| PubChem CID |
69505
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| Appearance |
Off-white to light brown solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
313.1±37.0 °C at 760 mmHg
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| Melting Point |
146-149 °C
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| Flash Point |
143.2±26.5 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.515
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| LogP |
1.07
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
13
|
| Complexity |
137
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
VTCDZPUMZAZMSB-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H12O4/c1-11-7-4-6(10)5-8(12-2)9(7)13-3/h4-5,10H,1-3H3
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| Chemical Name |
3,4,5-trimethoxyphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1357.29 mM)
H2O : ~2 mg/mL (~10.86 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (11.29 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (11.29 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (11.29 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 2 mg/mL (10.86 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4292 mL | 27.1459 mL | 54.2918 mL | |
| 5 mM | 1.0858 mL | 5.4292 mL | 10.8584 mL | |
| 10 mM | 0.5429 mL | 2.7146 mL | 5.4292 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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