| Size | Price | |
|---|---|---|
| 250mg | ||
| Other Sizes |
| Targets |
Angelic acid does not have a well-defined pharmacological target in the traditional sense. As a natural carboxylic acid, it is a plant metabolite and defense substance. It has been reported to have sedative and psychotropic properties and may aid in wound healing. The compound's biological effects are likely mediated through interactions with various cellular targets, though specific receptors or enzymes have not been identified.
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|---|---|
| ln Vitro |
In vitro, angelic acid has been studied for its sedative and psychotropic properties. As a carboxylic acid, its activity is related to its chemical properties rather than specific receptor interactions. The compound is used as a defense substance by beetles, suggesting antimicrobial or deterrent properties. Its effects on wound healing have been reported, though detailed in vitro data are limited.
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| ln Vivo |
In vivo, angelic acid has been reported to have sedative and psychotropic properties and may aid in wound healing. As a natural product found in various plants, it is consumed in the diet. However, detailed in vivo pharmacological data for angelic acid are limited in the available literature. The compound is primarily of interest as a natural product and chemical intermediate rather than as a therapeutic agent.
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| Enzyme Assay |
The in vitro assay for angelic acid depends on the specific biological activity being studied. For antimicrobial studies, angelic acid is tested against various bacterial or fungal strains using standard broth dilution or disk diffusion methods. The compound is dissolved in appropriate solvent and tested at varying concentrations (typically 0.1-1000 ug/mL). For studies of wound healing, cell migration assays using keratinocytes or fibroblasts can be performed. Cells are treated with angelic acid, and wound closure is monitored by microscopy. For sedative or psychotropic effects, receptor binding or neurotransmitter assays may be performed.
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| Cell Assay |
In vitro cellular assays for angelic acid are conducted using relevant cell lines depending on the research application. For wound healing studies, keratinocytes or fibroblasts are seeded in culture dishes and a scratch wound is created. Cells are treated with varying concentrations of angelic acid (typically 0.1-100 uM) for 24-72 hours, and wound closure is monitored by microscopy. Cell viability is assessed using MTT or similar assays. For studies of psychotropic effects, neuronal cell lines may be used, and neurotransmitter levels or receptor activation may be measured.
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| Animal Protocol |
In vivo animal studies for angelic acid are not extensively documented. As a natural product with reported sedative and psychotropic properties, typical studies would involve administration to rodents to assess behavioral effects. The compound would be administered by oral gavage or intraperitoneal injection at doses determined from preliminary studies. Behavioral tests such as the open field test, elevated plus maze, or forced swim test might be performed. However, detailed protocols are not available in the published literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of angelic acid are characteristic of a small organic acid. The compound has a molecular weight of 100.12 and a molecular formula of C5H8O2. It has a density of 0.96 g/cm3 and a melting point of 45degC. Angelic acid is soluble in hot water, alcohol, ether, and methanol, and sparingly soluble in cold water. It is sensitive to heat and light. As a small, polar molecule, it is expected to be well absorbed and rapidly metabolized.
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| Toxicity/Toxicokinetics |
The toxicological profile of angelic acid is characteristic of a naturally occurring organic acid. Despite its name, it is used as a defense substance by beetles and has a pungent odor and biting acid taste. The compound is generally considered to have low toxicity at normal dietary levels. Standard safety precautions should be followed when handling the compound in a laboratory setting. No specific LD50 values have been reported.
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| References | |
| Additional Infomation |
Angelic acid is the (Z)-isomer of 2-methylbut-2-enoic acid, found in plants of the Apiaceae family. It is a plant metabolite whose function is related to isocrotonic acid. Angelic acid has been reported in Lomatium nuttallii, Euryops arabicus, and other organisms with relevant data. See also: Roots (parts) of Petasites hybridus.
Angelic acid (CAS 565-63-9) is the (Z)-isomer of 2-methylbut-2-enoic acid found in various plants, particularly in the family Apiaceae. It is also known as cis-2,3-dimethylacrylic acid. Angelic acid has a pungent odor and biting acid taste and is used as a defense substance by beetles. It has been reported to have sedative and psychotropic properties and may aid in wound healing. The compound is available for research purposes only. |
| Molecular Formula |
C5H8O2
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|---|---|
| Molecular Weight |
100.1158
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| Exact Mass |
100.052
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| CAS # |
565-63-9
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| PubChem CID |
643915
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
198.5±0.0 °C at 760 mmHg
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| Melting Point |
44ºC
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| Flash Point |
95.9±9.6 °C
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| Vapour Pressure |
0.2±0.7 mmHg at 25°C
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| Index of Refraction |
1.451
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| LogP |
1.35
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
7
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| Complexity |
103
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C/C=C(/C)\C(=O)O
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| InChi Key |
UIERETOOQGIECD-ARJAWSKDSA-N
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| InChi Code |
InChI=1S/C5H8O2/c1-3-4(2)5(6)7/h3H,1-2H3,(H,6,7)/b4-3-
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| Chemical Name |
(Z)-2-methylbut-2-enoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~2497.00 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (20.78 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (20.78 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (20.78 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.9880 mL | 49.9401 mL | 99.8801 mL | |
| 5 mM | 1.9976 mL | 9.9880 mL | 19.9760 mL | |
| 10 mM | 0.9988 mL | 4.9940 mL | 9.9880 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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