| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents amino acid 25, and the second Gly Represents amino acid 25. The last Met stands for amino acid 35. Amyloid β-peptide (25-35) was also investigated in gel form for the first time. Comparative investigations were also undertaken using vibration absorption and ECD. The structural preferences of Aβ(25-35) peptide films were also examined using vibrational absorption and VCD spectroscopy [1]. Amyloid β-peptide (25-35) can trigger apoptosis in isolated brain mitochondria and methionine-35 redox state, playing a vital role in generating programmed cell death pathways and toxic events [2]. Recommendations for Beta-Amyloid Aggregation (This is our suggested approach; it should be adjusted to suit your particular need as it simply offers guidelines). 1. Solid Aβ peptide should be dissolved in cold hexafluoro-2-propanol (HFIP). To achieve monomerization and randomization of the structure, incubate the peptides for a minimum of one hour at room temperature. 2. To get rid of HFIP, evaporate, and then store the resultant peptide in a film at -20 or -80°C. 3. The resultant membrane should be dissolved in 5 mM of anhydrous DMSO, vortexed, and diluted with buffer (serum- and phenol-red-free media) to the proper concentration. 4. After that, let the solution age for 48 hours at 4–8°C. After centrifuging the samples for 10 minutes at 4–8°C at 14,000 g, the supernatant contained soluble oligomers. For the studies, the supernatant was diluted 10-200 times. Different approaches are used based on the final application.
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| ln Vivo |
Alzheimer's disease models can be created using animal models with β-Amyloid (25–35).
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| References |
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| Molecular Formula |
C45H81N13O14S
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|---|---|
| Molecular Weight |
1060.2683
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| Exact Mass |
1059.57
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| CAS # |
131602-53-4
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| PubChem CID |
3407255
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| Appearance |
White to off-white solid powder
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| Density |
1.249g/cm3
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| Boiling Point |
1517.336ºC at 760 mmHg
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| Flash Point |
871.453ºC
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| Vapour Pressure |
0mmHg at 25°C
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| Index of Refraction |
1.544
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| LogP |
1.058
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| Hydrogen Bond Donor Count |
15
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| Hydrogen Bond Acceptor Count |
17
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| Rotatable Bond Count |
37
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| Heavy Atom Count |
73
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| Complexity |
1880
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WIHBNMPFWRHGDF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C45H81N13O14S/c1-9-24(5)36(43(69)50-21-35(63)52-29(17-23(3)4)40(66)55-28(45(71)72)14-16-73-8)58-44(70)37(25(6)10-2)57-38(64)26(7)51-34(62)20-49-39(65)27(13-11-12-15-46)54-41(67)30(18-32(48)60)56-42(68)31(22-59)53-33(61)19-47/h23-31,36-37,59H,9-22,46-47H2,1-8H3,(H2,48,60)(H,49,65)(H,50,69)(H,51,62)(H,52,63)(H,53,61)(H,54,67)(H,55,66)(H,56,68)(H,57,64)(H,58,70)(H,71,72)
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| Chemical Name |
2-[[2-[[2-[[2-[[2-[2-[[2-[[6-amino-2-[[4-amino-2-[[2-[(2-aminoacetyl)amino]-3-hydroxypropanoyl]amino]-4-oxobutanoyl]amino]hexanoyl]amino]acetyl]amino]propanoylamino]-3-methylpentanoyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-4-methylpentanoyl]amino]-4-methylsulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product is not stable in solution, please use freshly prepared working solution for optimal results. (2). Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 3.33 mg/mL (~3.14 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9432 mL | 4.7158 mL | 9.4316 mL | |
| 5 mM | 0.1886 mL | 0.9432 mL | 1.8863 mL | |
| 10 mM | 0.0943 mL | 0.4716 mL | 0.9432 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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