| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
AMPPD does not have a pharmacological target, as it is a chemiluminescent substrate used for diagnostic purposes. It is specifically designed to be dephosphorylated by alkaline phosphatase, an enzyme commonly used as a label in immunoassays. The enzymatic dephosphorylation of AMPPD produces a stable intermediate that emits light.
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|---|---|
| ln Vitro |
In vitro, AMPPD is used as a substrate for alkaline phosphatase in chemiluminescent assays. The enzyme cleaves the phosphate group, producing a dioxetane anion that decomposes and emits light at 470 nm. The light emission is proportional to the amount of alkaline phosphatase present, allowing for highly sensitive detection.
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| ln Vivo |
AMPPD is not used in vivo as a pharmacological agent. It is a diagnostic reagent used in in vitro assays. It is not administered to animals or humans for therapeutic purposes.
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| Enzyme Assay |
The in vitro assay for AMPPD measures its chemiluminescent signal upon dephosphorylation by alkaline phosphatase. These cell-free assays use purified alkaline phosphatase or enzyme-labeled antibodies and measure the light emission using a luminometer. The signal intensity is proportional to the enzyme concentration, allowing for quantitative detection.
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| Cell Assay |
AMPPD is not used in cellular assays as a pharmacological agent. It is a diagnostic reagent. It may be used in cell-based immunoassays to detect alkaline phosphatase-labeled antibodies or other probes, but the substrate itself is not the subject of the assay.
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| Animal Protocol |
AMPPD is not used in animal studies, as it is a diagnostic reagent and not a pharmacological or therapeutic agent. Its applications are strictly limited to in vitro diagnostics and research.
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| ADME/Pharmacokinetics |
AMPPD is not administered systemically as it is not a drug. Therefore, pharmacokinetic properties such as absorption, distribution, metabolism, and excretion are not applicable. The compound is used in vitro as a reagent and is not intended for biological use.
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| Toxicity/Toxicokinetics |
AMPPD is a chemical reagent and is not intended for human or animal consumption. It may be irritating to skin, eyes, and respiratory tract. Standard laboratory safety precautions should be taken when handling this compound. Its toxicity profile is not extensively studied as it is not a pharmaceutical agent.
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| References |
J Chem Theory Comput. 2013 May 14;9(5):2300-12.
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| Additional Infomation |
AMPPD (CAS 122341-56-4) is a 1,2-dioxo-cyclohexane derivative and a supersensitive alkaline phosphatase substrate. It is a chemiluminescent substrate that emits light at 470 nm upon enzymatic dephosphorylation. It has a molecular formula of C18H23O7P and a molecular weight of 382.34. It is used in biochemical and clinical diagnostics.
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| Molecular Formula |
C18H23O7P
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|---|---|
| Molecular Weight |
382.3448
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| Exact Mass |
382.118
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| CAS # |
122341-56-4
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| PubChem CID |
64599
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
548.6±60.0 °C at 760 mmHg
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| Flash Point |
285.6±32.9 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.618
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| LogP |
1.99
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
591
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
XYIPYISRNJUPBA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H23O7P/c1-22-18(13-3-2-4-16(10-13)23-26(19,20)21)17(24-25-18)14-6-11-5-12(8-14)9-15(17)7-11/h2-4,10-12,14-15H,5-9H2,1H3,(H2,19,20,21)
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| Chemical Name |
[3-(3'-methoxyspiro[adamantane-2,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~83.33 mg/mL (~217.95 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (261.55 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6155 mL | 13.0774 mL | 26.1547 mL | |
| 5 mM | 0.5231 mL | 2.6155 mL | 5.2309 mL | |
| 10 mM | 0.2615 mL | 1.3077 mL | 2.6155 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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