| Size | Price | |
|---|---|---|
| Other Sizes |
Purity: ≥98%
| Targets |
Amino-PEG3-alcohol does not have a specific biological target, as it is a chemical linker. Its function is to serve as a covalent connector between molecular components. The primary amine group is a target for conjugation with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), and other electrophilic groups. The primary alcohol group can be further derivatized or substituted by other reactive functional groups. The hydrophilic PEG3 spacer enhances solubility in aqueous media and reduces steric hindrance between conjugated components.
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|---|---|
| ln Vitro |
Amino-PEG3-alcohol, as a linker, does not possess any direct in vitro biological activity, such as enzyme inhibition, receptor agonism, or cytotoxicity. Its function is to enable the synthesis of bioactive conjugates. The in vitro activity of this linker is assessed indirectly through the characterization of the final conjugates it helps to create. The efficiency of the conjugation reactions (amine coupling and alcohol activation) are the primary measures of this linker's performance in vitro.
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| ln Vivo |
Amino-PEG3-alcohol is not intended for in vivo use as a standalone compound. Its relevance in vivo is as a component of larger conjugates, such as PROTACs or ADCs. In these contexts, the hydrophilic PEG3 spacer contributes to the overall pharmacokinetic profile of the conjugate by improving solubility and reducing aggregation. However, the specific in vivo activity is determined by the conjugate's warhead and targeting ligand, not the linker itself. The linker's role is to stably connect the functional components.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for Amino-PEG3-alcohol, as it is a chemical reagent. Its characterization is performed using analytical chemistry techniques such as NMR, HPLC, and MS to verify its structure and purity. The functionality of the amine group can be confirmed by reacting it with a model NHS ester and monitoring the formation of the amide bond. The reactivity of the alcohol can be tested by converting it to a more reactive derivative. These are quality control steps, not biological assays.
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| Cell Assay |
There are no standard in vitro cell-based assays for Amino-PEG3-alcohol itself, as it is not a bioactive molecule. Its use in cell biology is indirect, as a reagent for constructing bioconjugates. For example, when used to create a PROTAC, the PROTAC's ability to degrade a target protein would be evaluated in cell-based assays. The linker's role is to ensure the PROTAC is functional and stable. However, there is no direct assay for the linker in cells.
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| Animal Protocol |
Amino-PEG3-alcohol is not used in in vivo animal experiments as a standalone compound. It is a synthetic building block for creating bioconjugates such as PROTACs or ADCs. Animal studies would be conducted on the final, larger conjugates that incorporate this linker. In such studies, the linker's contribution to the overall pharmacokinetic and pharmacodynamic profile of the conjugate might be assessed. However, there is no standard animal protocol for the linker itself.
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| ADME/Pharmacokinetics |
Amino-PEG3-alcohol has a molecular weight of 149.19 g/mol and a molecular formula of C6H15NO3. It has a CAS number of 6338-55-2. It is a solid or liquid compound with a purity of ≥95%. It is soluble in water and organic solvents. For storage, it is recommended to keep the compound at -20°C in a dry, dark environment. Detailed pharmacokinetic properties are not relevant as the compound is a linker, not a therapeutic agent.
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| Toxicity/Toxicokinetics |
Amino-PEG3-alcohol is considered to have low toxicity, consistent with other PEG-based linkers. PEG polymers are widely regarded as biocompatible, non-toxic, and non-immunogenic. The compound is classified as a research reagent, and standard laboratory safety precautions should be followed when handling it. Its toxicity profile has not been extensively studied, but due to its intended use and chemical class, it is not expected to be acutely toxic.
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| References |
Pignatello R, Impallomeni G, Pistarà V, Cupri S, Graziano AC, Cardile V, Ballistreri A. New amphiphilic derivatives of poly(ethylene glycol) (PEG) as surface modifiers of colloidal drug carriers. III. Lipoamino acid conjugates with carboxy- and amino-PEG(5000) polymers. Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:470-81. doi: 10.1016/j.msec.2014.10.054. Epub 2014 Oct 23. PubMed PMID: 25492012.
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| Additional Infomation |
Amino-PEG3-alcohol is a research-use-only reagent and is not a drug, nor is it approved for any clinical or therapeutic use. It is a heterobifunctional PEG linker that is used in bioconjugation and the synthesis of more complex molecules. It is a PROTAC linker of the polyethylene glycol (PEG) category that may be utilized to prepare PROTAC protein degraders. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The hydrophilic PEG spacer increases solubility in aqueous media. It is a valuable tool for medicinal chemists and chemical biologists working on targeted protein degradation and bioconjugation.
|
| Molecular Formula |
C6H15NO3
|
|---|---|
| Molecular Weight |
149.1882
|
| Exact Mass |
149.105
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| CAS # |
6338-55-2
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| Related CAS # |
6338-55-2;
|
| PubChem CID |
95875
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
253.8±20.0 °C at 760 mmHg
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| Flash Point |
107.3±21.8 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.455
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| LogP |
-1.73
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
10
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| Complexity |
61
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C([H])([H])C([H])([H])N([H])[H])C([H])([H])C([H])([H])OC([H])([H])C([H])([H])O[H]
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| InChi Key |
ASDQMECUMYIVBG-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H15NO3/c7-1-3-9-5-6-10-4-2-8/h8H,1-7H2
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| Chemical Name |
2-[2-(2-aminoethoxy)ethoxy]ethanol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~670.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 2.08 mg/mL (13.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (13.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7029 mL | 33.5143 mL | 67.0286 mL | |
| 5 mM | 1.3406 mL | 6.7029 mL | 13.4057 mL | |
| 10 mM | 0.6703 mL | 3.3514 mL | 6.7029 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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