| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
α-Tocotrienol does not have a single specific target but exerts its effects through multiple mechanisms. It functions as a potent antioxidant, protecting cells from oxidative stress. It also has a significant inhibitory effect on lipid peroxidation and plays a role in cholesterol biosynthesis inhibition. Its anticancer effects are mediated through the induction of apoptosis.
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| ln Vitro |
In vitro, α-tocotrienol displays cytotoxic activity against mouse +SA cells with an IC50 value of 5000.0 nM in a 24-hour MTT assay. It has been shown to inhibit cholesterol biosynthesis in isolated rat hepatocytes and HepG2 cells. It also induces apoptosis in cancer cells. Its strong antioxidative capacity has been demonstrated in various cellular models.
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| ln Vivo |
In vivo, α-tocotrienol is orally active and has demonstrated antitumor, neuroprotective, and antioxidant effects in animal models. It is absorbed and distributed throughout the body, where it exerts its biological activities. It is used in research to study the prevention and treatment of various diseases, including cancer, cardiovascular disease, and neurodegenerative disorders.
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| Enzyme Assay |
In vitro assays for α-tocotrienol typically measure its antioxidant capacity. This can be done using cell-free assays like the DPPH radical scavenging assay or ORAC (oxygen radical absorbance capacity) assay to quantify its ability to neutralize free radicals. Its effect on lipid peroxidation can be measured in isolated lipid systems or cell membranes.
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| Cell Assay |
In vitro cellular studies are conducted using various cell lines. For example, the MTT assay in mouse +SA cells is used to measure its cytotoxic activity. In HepG2 cells, its effect on cholesterol biosynthesis is studied. Cells are treated with α-tocotrienol, and endpoints such as cell viability, apoptosis, lipid levels, and oxidative stress markers are measured.
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| Animal Protocol |
In vivo animal experiments are performed in models of disease. For example, in cancer models, tumor-bearing mice are treated with α-tocotrienol to assess its antitumor effects. In neurodegenerative disease models, its neuroprotective effects are evaluated through behavioral tests and histological analysis. The compound is typically administered orally or via injection.
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| ADME/Pharmacokinetics |
α-Tocotrienol has a molecular formula of C29H44O2 and a molecular weight of 424.66 g/mol. It is a lipophilic compound with a density of approximately 0.94 g/mL. It is soluble in organic solvents like ethanol and DMSO but insoluble in water. For research, it is typically stored at -20°C, protected from light and air to prevent oxidation. Its purity is often >98%.
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| Toxicity/Toxicokinetics |
Toxicological data for α-tocotrienol are derived from its use as a dietary supplement and in research. It is generally considered safe and well-tolerated at standard doses. As a form of vitamin E, it has a low toxicity profile. However, high doses may have anticoagulant effects. Standard laboratory safety precautions should be followed when handling the compound.
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| Additional Infomation |
α-Tocotrienol is a tocotrienol with the structure chroman-6-ol, substituted with methyl groups at positions 2, 5, 7, and 8, and a farnesyl chain at position 2. It is found in the palm oil of oil palm (Elaeis guineensis). α-Tocotrienol has neuroprotective effects and is a plant metabolite, an inhibitor of ferroptosis, and a human metabolite. It is a tocotrienol and also a form of vitamin E. Tocotrienols have been investigated for their use in cholesterol-lowering treatments. α-Tocotrienol has been reported in amaranth (Amaranthus cruentus), humans (Homo sapiens), and other organisms with relevant data. Tocotrienols are any of the four forms (α, β, γ, and δ) of the vitamin E family and possess potential cholesterol-lowering, antithrombotic, antioxidant, immunomodulatory, and antitumor activities. Tocotrienols inhibit the activity of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, thereby lowering cholesterol levels. Furthermore, tocotrienols induce cell cycle arrest and caspase-mediated apoptosis through multiple signal transduction pathways and reduce tumor cell proliferation. This compound may also inhibit angiogenesis by blocking the vascular endothelial growth factor receptor (VEGFR) and subsequently inhibiting tumor cell-induced angiogenesis. Simultaneously, this compound can prevent free radical generation and inhibit lipid peroxidation, thereby preventing DNA damage. The farnesylisoprene side chain of tocotrienols contains three double bonds (a structure absent in tocopherols), which may be one reason for its anticancer activity.
α-Tocotrienol is a natural isoform of vitamin E found in various food sources. It is being investigated for its potential in cancer prevention, neuroprotection, and cardiovascular health. Its potent antioxidant and anti-inflammatory properties make it a subject of interest in research on aging and chronic diseases. It is available as a research-grade compound with high purity. |
| Molecular Formula |
C29H44O2
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|---|---|
| Molecular Weight |
424.65846
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| Exact Mass |
424.334
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| CAS # |
58864-81-6
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| PubChem CID |
5282347
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| Appearance |
Colorless to light yellow ointment
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
541.7±50.0 °C at 760 mmHg
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| Flash Point |
222.3±24.4 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.523
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| LogP |
10.76
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
31
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| Complexity |
654
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(=CCC/C(=C/CC/C(=C/CC[C@]1(C)CCC2=C(C)C(=C(C)C(=C2O1)C)O)/C)/C)C
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| InChi Key |
RZFHLOLGZPDCHJ-XZXLULOTSA-N
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| InChi Code |
InChI=1S/C29H44O2/c1-20(2)12-9-13-21(3)14-10-15-22(4)16-11-18-29(8)19-17-26-25(7)27(30)23(5)24(6)28(26)31-29/h12,14,16,30H,9-11,13,15,17-19H2,1-8H3/b21-14+,22-16+/t29-/m1/s1
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| Chemical Name |
(2R)-2,5,7,8-tetramethyl-2-[(3E,7E)-4,8,12-trimethyltrideca-3,7,11-trienyl]-3,4-dihydrochromen-6-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~235.48 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3548 mL | 11.7741 mL | 23.5483 mL | |
| 5 mM | 0.4710 mL | 2.3548 mL | 4.7097 mL | |
| 10 mM | 0.2355 mL | 1.1774 mL | 2.3548 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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