| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
(±)-α-Tocopherol acetate targets oxidative stress and free radicals. As a vitamin E derivative, it acts as a lipid-soluble antioxidant, protecting cell membranes from damage caused by free radicals. The compound's mechanism of action involves scavenging free radicals and preventing lipid peroxidation. (±)-α-Tocopherol acetate has oral activity and can be used in studies of fish susceptibility to infectious diseases. Its antioxidant properties make it a valuable tool for studying oxidative stress-related diseases and nutritional supplementation.
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| ln Vitro |
In vitro, (±)-α-Tocopherol acetate functions as a lipid-soluble antioxidant. It protects cells from oxidative damage by scavenging free radicals and preventing lipid peroxidation. The compound's antioxidant activity can be measured using various in vitro assays such as DPPH radical scavenging and lipid peroxidation inhibition assays. (±)-α-Tocopherol acetate is used as a vitamin E supplement and has been studied for its effects on heat-stress-induced decreases in blastocyst number.
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| ln Vivo |
(±)-alpha-tocopheryl acetate ((±)-vitamin E acetate) (oral; 600 mg/kg, 1200 mg/kg, 1800 mg/kg; 15, 30, and 45 days) exhibited a noteworthy level of complement activity [1]. (±)-α-Tocopherol acetate (600 mg/kg, 1200 mg/kg, 1800 mg/kg; 15, 30, and 45 days) after 30 days of in vivo fish administration method increases nonspecific immunity in sea bream. Consumption (1200 mg/kg) [1].
In vivo, (±)-α-Tocopherol acetate inhibits heat-stress-induced decreases in blastocyst number in pregnant mice when administered at a dose of 1 g/kg. It also inhibits heat-stress-induced decreases in glutathione levels in isolated mouse zygotes in a model of hyperthermia-induced pre-implantation embryonic death. The compound has oral activity and is used in studies of fish susceptibility to infectious diseases. Its antioxidant and cytoprotective effects make it a valuable compound for research on oxidative stress and reproductive biology. |
| Enzyme Assay |
In vitro experiments with (±)-α-Tocopherol acetate typically involve preparing stock solutions in ethanol or DMSO and diluting them in assay buffers or cell culture media. For antioxidant assays, the compound is incubated with free radical-generating systems, and the scavenging activity is measured. For cell-based studies, cells are treated with varying concentrations of the compound (1-100 µM) for 24-72 hours, and cell viability, oxidative stress markers, and signaling pathways are assessed. The compound is typically dissolved in ethanol or DMSO and diluted in cell culture medium.
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| Cell Assay |
In vitro cell-based assays using (±)-α-Tocopherol acetate are performed in various cell lines to study its antioxidant and cytoprotective effects. Cells are treated with the compound at concentrations of 1-100 µM for 24-72 hours. Oxidative stress is induced using agents such as hydrogen peroxide or heat stress. Cell viability is assessed using MTT or other cell viability assays. ROS levels are measured using fluorescent probes. The compound's ability to protect cells from oxidative damage is assessed by measuring cell viability and ROS levels. The compound is typically dissolved in ethanol or DMSO and diluted in cell culture medium.
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| Animal Protocol |
Animal/Disease Models: Gilthead seabream (Sparus aurata L.) [1]
Doses: 600 mg/kg, 1200 mg/kg, 1800 mg/kg Route of Administration: oral; 600 mg/kg, 1200 mg/kg, 1800 mg/kg kg; 15-day, 30-day and 45-day Experimental Results: positively correlated with serum α-tocopherol levels, and the increase is related to dose and time of use. They did not show an enhancement in any immune parameter, while the specific growth rate of fish fed the diet supplemented with 1200 mg/kg was slightly higher (but not statistically significant) than that of fish fed the other diets. After 30 and 45 days, dietary intake of 1200 mg/kg enhanced serum hemolytic activity and phagocytosis of head kidney leukocytes, but leukocyte migration and respiratory burst activity were not affected. Does not cause immune stimulation at 1800 mg/kg. In vivo animal experiments with (±)-α-Tocopherol acetate have been conducted in pregnant mice to study its effects on heat-stress-induced embryonic death. The compound was administered orally at a dose of 1 g/kg. The number of blastocysts and glutathione levels in isolated mouse zygotes were measured as markers of embryonic development and oxidative stress. The compound's protective effects against heat stress were assessed. The compound has also been used in studies of fish susceptibility to infectious diseases. |
| ADME/Pharmacokinetics |
(±)-α-Tocopherol acetate has a molecular weight of 472.75 g/mol and the formula C31H52O3. The compound is soluble in DMSO at 100 mg/mL. For storage, the compound is kept at room temperature in a dry place, protected from light. It is an acetate form of (±)-α-tocopherol and an active form of vitamin E. The compound is a lipid-soluble antioxidant found in whole grains, nuts, and oils. It is used as a vitamin E supplement.
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| Toxicity/Toxicokinetics |
Specific toxicological data for (±)-α-Tocopherol acetate are not extensively provided. As a vitamin E derivative, it is generally considered to have low toxicity. However, at very high doses, vitamin E can have adverse effects. The compound is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection.
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| References | |
| Additional Infomation |
DL-α-Tocopherol acetate is a tocopherol. It has been reported that DL-α-tocopherol acetate is found in catechu (Senegalia catechu), and relevant data exists. Vitamin E acetate is an acetate of the fat-soluble vitamin E, possessing potent antioxidant properties. Vitamin E is considered essential for stabilizing biological membranes, especially those rich in polyunsaturated fatty acids. It is a collective term for tocopherols and tocotrienols (collectively known as tocopherols). Vitamin E is a potent superoxide radical scavenger, capable of non-competitively inhibiting cyclooxygenase activity in various tissues, thereby reducing prostaglandin production. Vitamin E can also inhibit angiogenesis and tumor dormancy by inhibiting vascular endothelial growth factor (VEGF) gene transcription. (NCI04) It is a natural tocopherol and one of the most effective antioxidants. α-Tocopherol possesses antioxidant activity due to the phenolic hydroxyl group on its 2H-1-benzopyran-6-ol core. Its 6-chromoyl alcohol core has four methyl groups. Natural d-α-tocopherol is more active than its synthetic racemic mixture of dl-α-tocopherol.
See also: α-tocopherol acetate (note moved here). (±)-α-Tocopherol acetate (CAS 7695-91-2) is an acetate form of (±)-α-tocopherol and an active form of vitamin E. It has the molecular formula C31H52O3 and a molecular weight of 472.75 g/mol. The compound is a lipid-soluble antioxidant found in whole grains, nuts, and oils. (±)-α-Tocopherol acetate is used as a vitamin E supplement and has oral activity. It inhibits heat-stress-induced decreases in blastocyst number in pregnant mice when administered at a dose of 1 g/kg. The compound is also used in studies of fish susceptibility to infectious diseases. It is intended for research use only. |
| Molecular Formula |
C31H52O3
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|---|---|
| Molecular Weight |
472.7428
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| Exact Mass |
472.391
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| CAS # |
7695-91-2
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| Related CAS # |
D-α-Tocopherol acetate;58-95-7;DL-α-Tocopherol acetate;52225-20-4
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| PubChem CID |
2117
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| Appearance |
Colorless to light yellow viscous liquid
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
485.3±0.0 °C at 760 mmHg
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| Melting Point |
82 °F (NTP, 1992)
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| Flash Point |
235.6±24.7 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.488
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| LogP |
12.07
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
34
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| Complexity |
602
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| Defined Atom Stereocenter Count |
0
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| SMILES |
0
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| InChi Key |
ZAKOWWREFLAJOT-CEFNRUSXSA-N
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| InChi Code |
InChI=1S/C31H52O3/c1-21(2)13-10-14-22(3)15-11-16-23(4)17-12-19-31(9)20-18-28-26(7)29(33-27(8)32)24(5)25(6)30(28)34-31/h21-23H,10-20H2,1-9H3/t22-,23-,31-/m1/s1
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| Chemical Name |
[(2R)-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-3,4-dihydrochromen-6-yl] acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~211.53 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (5.29 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (5.29 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.29 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1153 mL | 10.5766 mL | 21.1533 mL | |
| 5 mM | 0.4231 mL | 2.1153 mL | 4.2307 mL | |
| 10 mM | 0.2115 mL | 1.0577 mL | 2.1153 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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