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250mg |
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Purity: ≥98%
Aloe emodin (also known as NSC 38628, Rhabarberone; 3-Hydroxymethylchrysazine) is a hydroxyanthraquinone based and naturally occuring CFTR channel activator found in Aloe vera leaves, and has a specific in vitro and in vivo antitumor activity. It is an interferon-inducing agent with IC50 of about 1 μg/mL for JEV and of about 0.33 μg/mL for EV71. Aloe-emodin shows significant inhibitory activity against the P-388 leukemia in mice when administered as a suspension in acetone-Tween 80. Has a specific in vitro and in vivo antineuroectodermal tumor activity. Aloe-emodin treatment led to the dissociation of heat shock protein 90 (HSP90) and ER α and increased ER α ubiquitination.
ln Vitro |
In a dose-dependent manner, aloe emodin (0–15 μM; 24-96 hours) suppresses PC3 cell proliferation [1]. Aloe emodin (0–15 μM; 24 hours) prevents Akt and PKCα, two downstream substrates of mTORC2, from activating. Aloe emodin has the ability to attach itself to intracellular mTORC2 and stop kinase activation [1].
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ln Vivo |
Through the inhibition of mTOR complex 2 activity, aloe emodin (10–50 mg/kg) suppresses the growth of tumors [1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: PC3 cells Tested Concentrations: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM Incubation Duration: 24 h, 48 h, 72 h or 96 h Experimental Results: Suppressed proliferation and anchorage-independent growth of PC3 cells. Western Blot Analysis[1] Cell Types: PC3 cells Tested Concentrations: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM Incubation Duration: 24 h Experimental Results: Inhibited the activation of the downstream substrates of mTORC2, Akt and PKCα. |
Animal Protocol |
Animal/Disease Models: Athymic nude mice (BALB/c nude mouse, 6 weeks old)[1]
Doses: 10 mg/kg, 50 mg/kg (20% PEG400 in autoclaved PBS) Route of Administration: intraperitoneal (ip)injection, five times per week; for 28 days Experimental Results: demonstrated tumor suppression effects in vivo in an athymic nude mouse model. |
References |
[1]. Liu K, et al. Aloe-emodin suppresses prostate cancer by targeting the mTOR complex 2. Carcinogenesis. 2012 Jul;33(7):1406-11.
[2]. Li SW, et al. Antiviral activity of aloe-emodin against influenza A virus via galectin-3 up-regulation. Eur J Pharmacol. 2014 Sep 5;738:125-32. |
Molecular Formula |
C15H10O5
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Molecular Weight |
270.24
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CAS # |
481-72-1
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Related CAS # |
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SMILES |
O([H])C1=C([H])C(C([H])([H])O[H])=C([H])C2C(C3C([H])=C([H])C([H])=C(C=3C(C=21)=O)O[H])=O
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InChi Key |
YDQWDHRMZQUTBA-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C15H10O5/c16-6-7-4-9-13(11(18)5-7)15(20)12-8(14(9)19)2-1-3-10(12)17/h1-5,16-18H,6H2
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Chemical Name |
1,8-dihydroxy-3-(hydroxymethyl)anthracene-9,10-dione
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Synonyms |
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (7.70 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (7.70 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL | |
5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | |
10 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.