| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
Calcium uptake, microsomal calcium binding, and Ca2+-stimulated Mg2+-dependent ATPase activity are all decreased by adrenochrome. There is no correlation between pH (6.0-8.0), calcium concentration (10-200 μM), protein concentration (0.02-0.10 mg/mL), temperature (25-37 degrees C), and incubation period (2-30 minutes) and the inhibitory impact of adrenochrome on the calcium uptake activity of separation membrane microsomes [2].
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| ln Vivo |
Adrenochrome concentrations ranging from 1 to 1000 ng/mL induced dose- and time-dependent increases in coronary artery pressure in isolated rat hearts. Moreover, the concentration of CaCl2 in the perfusion medium determines the degree of adrenochrome contraction [1].
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| References |
[1]. M Karmazyn,, et al. Adrenochrome-induced coronary artery constriction in the rat heart. J Pharmacol Exp Ther. 1981 Oct;219(1):225-30.
[2]. S Takeo,et al. Effects of adrenochrome on calcium accumulating and adenosine triphosphatase activities of the rat heart microsomes. J Pharmacol Exp Ther. 1980 Sep;214(3):688-93. [3]. Koji Ueda, et al. Catecholamine oxidation-mediated transcriptional inhibition in Mn neurotoxicity. J Toxicol Sci. 2020;45(10):619-624. |
| Additional Infomation |
Adrenochrome is a member of indoles.
Pigment obtained by the oxidation of epinephrine. |
| Molecular Formula |
C9H9NO3
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|---|---|
| Molecular Weight |
179.18
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| Exact Mass |
179.058
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| Elemental Analysis |
C, 60.33; H, 5.06; N, 7.82; O, 26.79
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| CAS # |
54-06-8
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| PubChem CID |
5898
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| Appearance |
Solid powder
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| Density |
1.42g/cm3
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| Boiling Point |
375.1ºC at 760 mmHg
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| Melting Point |
115-120ºC
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| Flash Point |
180.7ºC
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| Index of Refraction |
1.63
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| LogP |
-1.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
13
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| Complexity |
354
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN1CC(C2=CC(=O)C(=O)C=C21)O
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| InChi Key |
RPHLQSHHTJORHI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H9NO3/c1-10-4-9(13)5-2-7(11)8(12)3-6(5)10/h2-3,9,13H,4H2,1H3
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| Chemical Name |
3-hydroxy-1-methyl-2,3-dihydroindole-5,6-dione
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| Synonyms |
Adrenochrome Adraxone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~558.13 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (13.95 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (13.95 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5810 mL | 27.9049 mL | 55.8098 mL | |
| 5 mM | 1.1162 mL | 5.5810 mL | 11.1620 mL | |
| 10 mM | 0.5581 mL | 2.7905 mL | 5.5810 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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