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|---|---|---|
| Other Sizes |
| Targets |
ADPS is a chromogenic reagent used in enzyme-coupled assays for the detection of hydrogen peroxide (H₂O₂). It does not have a specific biological target but reacts with H₂O₂ in the presence of peroxidase to produce a colored product.
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|---|---|
| ln Vitro |
In biochemical assays, ADPS reacts with hydrogen peroxide in the presence of peroxidase to generate a colored product that can be measured spectrophotometrically. The compound has high water solubility and a molar absorption coefficient of ≥8200 at λmax 255 nm. It is used as a Trinder's reagent in clinical chemistry.
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| ln Vivo |
Cells are not typically treated with ADPS in biological assays; rather, the compound is used as a reagent in cell-free diagnostic systems. It is employed in assays for measuring glucose, cholesterol, uric acid, and other analytes that generate H₂O₂.
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| Enzyme Assay |
The Trinder reaction assay involves incubating ADPS with a sample containing H₂O₂ and horseradish peroxidase (HRP). The reaction produces a colored product that is measured spectrophotometrically at the appropriate wavelength. ADPS is typically used at concentrations of 0.1-10 mM in assay buffer. The reaction is linear over a wide range of H₂O₂ concentrations.
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| Cell Assay |
Cell-free diagnostic assays use ADPS as a chromogenic substrate. The compound is dissolved in assay buffer and combined with HRP and the sample. The reaction is incubated at 37°C for a defined period, and absorbance is measured using a plate reader or spectrophotometer.
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| Animal Protocol |
In vivo studies are not typically conducted with ADPS as it is a diagnostic reagent rather than a therapeutic compound. The compound is used exclusively in in vitro diagnostic applications.
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| ADME/Pharmacokinetics |
ADPS (C₁₂H₁₈NNaO₄S, MW 295.33) is a highly water-soluble compound. It is stable as a white to slightly brown powder and is typically stored at 2-8°C. The compound has a purity of ≥99%.
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| Toxicity/Toxicokinetics |
ADPS is considered a safe laboratory reagent with no significant toxicity at concentrations used in diagnostic assays. Standard laboratory safety precautions should be followed when handling the compound.
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| Additional Infomation |
ADPS (N-ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt) is a novel Trinder's reagent used in clinical chemistry and biochemical diagnostics. It is a water-soluble modified Trinder reagent with high sensitivity for hydrogen peroxide detection. The compound is used in assays for measuring various analytes including glucose, cholesterol, uric acid, and triglycerides.
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| Molecular Formula |
C12H21NNAO5S
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|---|---|
| Molecular Weight |
314.3536
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| Exact Mass |
295.085
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| CAS # |
82611-88-9
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| PubChem CID |
23709986
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| Appearance |
White to gray solid powder
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| LogP |
2.537
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
19
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| Complexity |
331
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[Na].O=S(CCCN(CC)C1C=C(OC)C=CC=1)(O)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~338.60 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (338.60 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1812 mL | 15.9058 mL | 31.8117 mL | |
| 5 mM | 0.6362 mL | 3.1812 mL | 6.3623 mL | |
| 10 mM | 0.3181 mL | 1.5906 mL | 3.1812 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT04647773 | UNKNOWN STATUS | Drug: HSK16149 20mg BID Drug: HSK16149 40mg BID Drug: HSK16149 60mg BID |
Diabetic Peripheral Neuropathic Pain | Haisco Pharmaceutical Group Co., Ltd. | 2020-12-02 | Phase 2 Phase 3 |
| NCT05140863 | COMPLETED | Drug: HSK16149 20mg BID Drug: HSK16149 40mg BID Drug: Placebo BID |
Postherpetic Neuralgia | Haisco Pharmaceutical Group Co., Ltd. | 2021-11-01 | Phase 3 |
| NCT02607280 | COMPLETEDWITH RESULTS | Drug: DS-5565 | Diabetic Peripheral Neuropathic Pain Post-herpetic Neuralgia | Daiichi Sankyo Co., Ltd. | 2015-12 | Phase 3 |
| NCT02318706 | COMPLETEDWITH RESULTS | Drug: DS-5565 Drug: placebo |
Diabetic Peripheral Neuropathic Pain | Daiichi Sankyo Co., Ltd. | 2015-01 | Phase 3 |
| NCT02318719 | COMPLETEDWITH RESULTS | Drug: Placebo Drug: DS-5565 |
Post-Herpetic Neuralgia | Daiichi Sankyo Co., Ltd. | 2015-01 | Not Applicable |
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