| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
Acid blue 3 does not have a defined biological target as it is a dye rather than a pharmaceutical agent. It is used for its coloring properties as a food additive (E131) and as a dye in textile and biological staining applications. Its mechanism of action is physical, based on its color properties and ability to bind to biological tissues for staining purposes.
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|---|---|
| ln Vitro |
Acid blue 3 is not a bioactive compound with defined pharmacological activity. Its in vitro activity is related to its use as a pH indicator and a dye for staining biological specimens. The compound changes color in response to pH changes and binds to proteins and other biological molecules, making it useful for histological and cytological staining applications.
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| ln Vivo |
Acid blue 3 is not used as a therapeutic agent and does not have defined in vivo pharmacological activity. It is a food additive and industrial dye used for coloring purposes. The compound is not intended for in vivo use as a drug. Its applications are limited to food, cosmetic, and laboratory settings.
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| Enzyme Assay |
In vitro assays for Acid blue 3 typically involve measuring its color properties, including absorption spectra and color stability. The compound is dissolved in appropriate solvents, and UV-Vis spectra are recorded. Its stability under various conditions (pH, temperature, light) is assessed. Protein binding studies may be performed to characterize its staining properties for histological applications.
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| Cell Assay |
Cell-based assays are not applicable for Acid blue 3 as it is not a bioactive compound. The compound is used as a dye for staining biological specimens in histology and microscopy. It is not intended for use in cellular assays or as a therapeutic agent. Its potential cytotoxicity may be assessed in cell culture for safety evaluation purposes.
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| Animal Protocol |
In vivo animal studies are not applicable for Acid blue 3 as it is not a therapeutic agent. The compound is a food additive and industrial dye used for coloring purposes. Toxicological studies have been conducted to assess its safety as a food colorant in animal models, but these are not standard pharmacological studies.
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| ADME/Pharmacokinetics |
Acid blue 3 has a molecular weight of 1159.42 g/mol and a molecular formula of (C27H31N2O7S2)2Ca. It appears as a dark bluish powder. The compound is soluble in water and should be stored under dry conditions protected from light. It is used as a food coloring agent (E131) and as a biological stain.
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| Toxicity/Toxicokinetics |
Acid blue 3 (Patent Blue V) is generally recognized as safe for use as a food colorant at approved concentrations. It has been evaluated for safety by regulatory authorities. The compound may cause allergic reactions in some individuals. It is not intended for therapeutic use. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
CI Acid Blue 3 is a diarylmethane.
Acid blue 3 (Patent Blue V calcium salt, CI 42051, E131) is a synthetic triphenylmethane dye used as a food coloring agent and biological stain. It is a calcium-chelated dimer with a molecular formula of (C27H31N2O7S2)2Ca. The compound is not a therapeutic agent and is intended for use as a colorant in food, cosmetics, and research applications. |
| Molecular Formula |
C54H62CAN4O14S4
|
|---|---|
| Molecular Weight |
1159.42
|
| Exact Mass |
1158.277
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| CAS # |
3536-49-0
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| PubChem CID |
77073
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| Appearance |
Brown to black solid powder
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| Melting Point |
>300ºC
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| LogP |
11.258
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
14
|
| Heavy Atom Count |
77
|
| Complexity |
1070
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
RTMBGDBBDQKNNZ-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/2C27H32N2O7S2.Ca/c2*1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33/h2*9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36)/q+2/p-2
|
| Chemical Name |
Bis(hydrogen (4-(4-(diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium), calcium salt
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| Synonyms |
Acid blue 3 Food Blue 3C.I. 42051 L-Blau 3NSC 5010Blue ZN 3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~143.49 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.58 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8625 mL | 4.3125 mL | 8.6250 mL | |
| 5 mM | 0.1725 mL | 0.8625 mL | 1.7250 mL | |
| 10 mM | 0.0863 mL | 0.4313 mL | 0.8625 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT00003654 | Terminated | Drug: patent blue V dye Procedure: lymphangiography |
Breast Cancer | UNICANCER | September 1997 | Not Applicable |
| NCT00052676 | Completed | Drug: patent blue V dye Procedure: radionuclide imaging |
Breast Cancer | Centre Paul Strauss | October 2002 | Not Applicable |
| NCT04879680 | Completed | Procedure: sentinel node detection | Breast Cancer Sentinel Lymph Node |
Faculty of Medicine, Sousse | April 2016 | |
| NCT03476499 | Completed | Device: Near Infrared Imaging (NIR) device |
Flap Necrosis | University Health Network, Toronto | October 29, 2018 | Not Applicable |
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