| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
N-acetylcysteine amide exhibited considerable cytotoxicity at 10–20 mM, although it had no discernible influence on the viability of H9c2 cells treated with <1 mM doxorubicin (DOX). N-acetyl cysteine amide (750 μM) decreases ROS levels and lipid peroxidation caused by DOX, and it also restores the GSH/GSSG ratio and antioxidant enzyme activity, including catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) [1]. Methamphetamine (METH)-induced cell death is prevented in human brain microvascular endothelium (HBMVEC) by N-acetylcysteine amide (1 mM) [3].
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| ln Vivo |
The central nervous system is more bioavailable when N-acetylcysteine amide is present. In rats with traumatic brain injury (TBI), N-acetylcysteine amide (150 mg/kg, i.p.) increases mitochondrial bioenergetics, decreases oxidative stress, preserves mitochondrial trough, and enhances cortical protection and functional outcomes[2].
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| References |
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| Additional Infomation |
N-Acetylcysteine Amide is the amide form of N-acetylcysteine (NAC), a synthetic N-acetyl derivative and prodrug of the endogenous amino acid and antioxidant glutathione (GSH) precursor L-cysteine, with potential antioxidant and anti-inflammatory activities. Upon administration, N-acetylcysteine amide (NACA) increases GSH levels. GSH scavenges reactive oxygen species (ROS), reduces oxidative stress and prevents ROS-mediated cell damage and apoptosis. NACA has enhanced lipophilicity and membrane permeability compared with NAC.
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| Molecular Formula |
C5H10N2O2S
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|---|---|
| Molecular Weight |
162.2101
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| Exact Mass |
162.046
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| CAS # |
38520-57-9
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| PubChem CID |
10176265
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| Appearance |
White to off-white solid powder
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| LogP |
0.896
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
10
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| Complexity |
149
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(N[C@@H](CS)C(N)=O)=O
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| InChi Key |
UJCHIZDEQZMODR-BYPYZUCNSA-N
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| InChi Code |
InChI=1S/C5H10N2O2S/c1-3(8)7-4(2-10)5(6)9/h4,10H,2H2,1H3,(H2,6,9)(H,7,8)/t4-/m0/s1
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| Chemical Name |
(2R)-2-acetamido-3-sulfanylpropanamide
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| Synonyms |
NACANac amide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~200 mg/mL (~1232.97 mM)
DMSO : ≥ 100 mg/mL (~616.48 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (15.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (15.41 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (15.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (616.48 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.1648 mL | 30.8242 mL | 61.6485 mL | |
| 5 mM | 1.2330 mL | 6.1648 mL | 12.3297 mL | |
| 10 mM | 0.6165 mL | 3.0824 mL | 6.1648 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT05994534 | RECRUITING | Drug: Cysteamine Bitartrate Drug: N-Acetylcysteine Amide |
Cystinosis | Nacuity Pharmaceuticals, Inc. | 2023-10-29 | Phase 1 Phase 2 |
| NCT06169280 | NOT YET RECRUITING | Biological: NSC-CRAd-S-pk7 Dietary Supplement: N-acetylcysteine amide (NACA) |
Glioma, Malignant New Diagnosis Tumor |
Northwestern University | 2024-06-01 | Phase 1 |
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