| ln Vitro |
N-acetyl-L-glutamic acid significantly increases cell density in animal cell culture, sustains higher cell viability over culture time, and enhances antibody production in antibody-producing cells beyond the effect of cell number increase alone[1]
N-acetyl-L-glutamic acid at 10–50 mg/L promotes cell growth exceeding the saturation density of basal IMDM medium and maintains cell density for a longer culture period[1] |
|---|---|
| Cell Assay |
N-acetyl-L-glutamic acid significantly increases cell density in animal cell culture, sustains higher cell viability over culture time, and enhances antibody production in antibody-producing cells beyond the effect of cell number increase alone[1]
N-acetyl-L-glutamic acid at 10–50 mg/L promotes cell growth exceeding the saturation density of basal IMDM medium and maintains cell density for a longer culture period[1] |
| References | |
| Additional Infomation |
N-acetyl-L-glutamic acid is an N-acyl-L-amino acid, a derivative of L-glutamic acid where one amino hydrogen atom is replaced by an acetyl group. It is a metabolite of both Saccharomyces cerevisiae and the human body. It is both an N-acetyl-L-amino acid and an N-acyl-L-glutamic acid. Functionally, it is related to L-glutamic acid. It is the conjugate acid of N-acetyl-L-glutamic acid (1-). N-acetyl-L-glutamic acid is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). It has also been reported that N-acetyl-L-glutamic acid is present in Hypericum perforatum, soybeans, and some other organisms with relevant data. N-acetyl-L-glutamic acid is a metabolite found or produced in Saccharomyces cerevisiae.
N-acetyl-L-glutamic acid is used as a cell growth promoter and antibody production enhancer in serum-free, low-protein, or protein-free animal cell culture media[1] The effective concentration of N-acetyl-L-glutamic acid in culture medium is 1–200 mg/L, with optimal effect at 10–100 mg/L; the effect plateaus above 50 mg/L[1] N-acetyl-L-glutamic acid participates in the urea cycle (ornithine cycle) carbamoyl phosphate synthesis, promoting nitrogen metabolism to boost cell growth[1] N-acetyl-L-glutamic acid can be autoclaved with basal medium due to its high thermal stability[1] Combination of N-acetyl-L-glutamic acid with D-penicillamine expands the range of culturable cell density[1] |
| Molecular Formula |
C7H11NO5
|
|---|---|
| Molecular Weight |
189.1659
|
| Exact Mass |
189.063
|
| CAS # |
1188-37-0
|
| PubChem CID |
70914
|
| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
495.9±35.0 °C at 760 mmHg
|
| Melting Point |
194-196 °C(lit.)
|
| Flash Point |
253.7±25.9 °C
|
| Vapour Pressure |
0.0±2.7 mmHg at 25°C
|
| Index of Refraction |
1.502
|
| LogP |
-2.24
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
13
|
| Complexity |
225
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
CC(=O)N[C@@H](CCC(=O)O)C(=O)O
|
| InChi Key |
RFMMMVDNIPUKGG-YFKPBYRVSA-N
|
| InChi Code |
InChI=1S/C7H11NO5/c1-4(9)8-5(7(12)13)2-3-6(10)11/h5H,2-3H2,1H3,(H,8,9)(H,10,11)(H,12,13)/t5-/m0/s1
|
| Chemical Name |
(2S)-2-acetamidopentanedioic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~528.63 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2863 mL | 26.4313 mL | 52.8625 mL | |
| 5 mM | 1.0573 mL | 5.2863 mL | 10.5725 mL | |
| 10 mM | 0.5286 mL | 2.6431 mL | 5.2863 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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