| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
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|---|---|
| Enzyme Assay |
Transacylase assay: The reaction mixture for transacylase assay contained tris-HCl buffer pH 8.3 (100 μmol), N-acetyl-L-amino acid (10 μmol for L-isomer or 20 μmol for DL-isomer), DL-[1-14C]phenylalanine (8×10^4 c.p.m.) or L-[U-14C]phenylalanine (4×10^4 c.p.m.), and transacylase (400-500 μg protein) in 1 ml final volume. Incubation was at 37°C for 15 min. The reaction was terminated by boiling for 3 min, centrifuged, and the supernatant applied to a Dowex 50 (H+ form) column (1 cm × 4 cm), eluted with water, and the effluent radioactivity counted. One unit of enzyme activity is defined as the amount catalyzing formation of 1 μmol of N-acetyl-L-[14C]phenylalanine under these conditions. [1]
Acetyl-CoA-L-phenylalanine α-N-acetyltransferase assay: The reaction mixture contained (in 1 ml): tris-HCl buffer pH 8.3 (100 μmol), L-phenylalanine (10 μmol) containing L-[U-14C]phenylalanine (4×10^4 c.p.m.) or DL-[1-14C]phenylalanine (8×10^4 c.p.m.), acetyl-CoA (100 μg), and purified acetyltransferase (10-100 μg). Incubation at 37°C for 15 min, terminated by boiling for 3 min, centrifuged, supernatant applied to Dowex 50 (H+ form) column (1 cm × 4 cm), eluted with water to 25 ml, and radioactivity counted. One unit of activity is the amount catalyzing formation of 1 μmol of N-Acetyl-L-Phenylalanine per min. [1] Reversibility assay: Reaction mixture (1 ml) contained TME buffer (50 μmol), L-phenylalanine (20 μmol), N-acetyl-DL-[1-14C]phenylalanine (6×10^4 c.p.m.), MgCl2 (10 μmol), CoA (100 μg), and α-N-acetyltransferase (100 μg). Incubation at 37°C for 15 min, stopped by heat, centrifuged, supernatant applied to Dowex 50 (H+ form) column (1 cm × 5 cm), washed with water (25 ml), then amino acids eluted with 2 M NH3 (25 ml). Eluates concentrated and radioactivity counted. No reversibility was detected. [1] |
| References | |
| Additional Infomation |
N-acetyl-L-phenylalanine is an N-acetyl derivative of L-phenylalanine and a metabolite. It belongs to the N-acyl-L-phenylalanine, N-acetyl-L-amino acid, and N-acetylphenylalanine families. It is the conjugate acid of N-acetyl-L-phenylalanine and an enantiomer of N-acetyl-D-phenylalanine. N-acetyl-L-phenylalanine has been reported in Daphnia pulex, Bombyx mori, and other organisms with relevant data. N-acetyl-D-phenylalanine is a metabolite found or produced in Saccharomyces cerevisiae. See also: aflanin (note moved here).
N-Acetyl-L-Phenylalanine was identified as the principal acylamino acid in Escherichia coli K12 both free and in proteins. [1] The compound is synthesized from acetyl-CoA and L-phenylalanine by α-N-acetyltransferase, and also participates in transacylation reactions with transacylase. [1] The purified α-N-acetyltransferase had pH optimum 8, Km for L-phenylalanine 3.3 mM and for acetyl-CoA 0.13 mM. [1] The enzyme is a thiol enzyme inhibited by p-hydroxymercuribenzoate, N-ethylmaleimide, and iodoacetamide (0.08 mM gives 80-90% inhibition). [1] |
| Molecular Formula |
C11H13NO3
|
|---|---|
| Molecular Weight |
207.2258
|
| Exact Mass |
207.089
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| CAS # |
2018-61-3
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| PubChem CID |
74839
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| Appearance |
White to off-white powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
453.9±38.0 °C at 760 mmHg
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| Melting Point |
171-173 °C(lit.)
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| Flash Point |
228.3±26.8 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.548
|
| LogP |
0.78
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
15
|
| Complexity |
234
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
O([H])C([C@]([H])(C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H])N([H])C(C([H])([H])[H])=O)=O
|
| InChi Key |
CBQJSKKFNMDLON-JTQLQIEISA-N
|
| InChi Code |
InChI=1S/C11H13NO3/c1-8(13)12-10(11(14)15)7-9-5-3-2-4-6-9/h2-6,10H,7H2,1H3,(H,12,13)(H,14,15)/t10-/m0/s1
|
| Chemical Name |
(2S)-2-acetamido-3-phenylpropanoic acid
|
| Synonyms |
N-Acetylphenylalanine; N-Acetyl-L-phenylalanine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~482.56 mM)
H2O : ~7.69 mg/mL (~37.11 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.06 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.06 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.06 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 7.14 mg/mL (34.45 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8256 mL | 24.1278 mL | 48.2556 mL | |
| 5 mM | 0.9651 mL | 4.8256 mL | 9.6511 mL | |
| 10 mM | 0.4826 mL | 2.4128 mL | 4.8256 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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