| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
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| Other Sizes |
|
| Targets |
P2Y6 Receptor
|
|---|---|
| ln Vitro |
Microglia dramatically upregulates the expression of CCL2 and CCL3 mRNA when exposed to uridine-5'-diphosphate disodium salt (100 μM; 15 min). Microglia are induced to express chemokines by uridine-5'-diphosphate disodium salt (100 μM; 3 hours) [2]. In 30 minutes, mRNA encoding CCL2 and CCL3 is induced to express; this expression reaches a maximum level at 1 hour and returns to the base level after 3–12 hours when uridine-5'-diphosphate disodium salt (100 μM; 0.5, 1, 3, 6 12 hours) is added. Chemokine expression is increased concentration-dependently at the mRNA and protein levels when uridine-5'-diphosphate disodium salt (10, 100, and 1000 μM) is added during a three-hour period. Microglia exhibit activation of NFATc1 and NFATc2 with exposure to 100 μM uridine-5'-diphosphate disodium salt for 15 minutes [2]. Although it is not an antagonist of the rat P2Y14 receptor, uridine-5'-diphosphate disodium salt is a competitive antagonist of the human P2Y14 receptor (pEC50=7.28) [1]. Strong P2Y14 agonist UDP-glucose (UDPG; HY-N7032) [3][4].
|
| Cell Assay |
RT-PCR[2]
Cell Types: Primary microglia Tested Concentrations: 100 μM Incubation Duration: For 15 min Experimental Results: Dramatically induced microglial CCL2 and CCL3 mRNA expression. |
| References |
|
| Additional Infomation |
UDP is a uridine 5'-phosphate and a pyrimidine ribonucleoside 5'-diphosphate. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a conjugate acid of an UDP(3-).
Uridine 5'-diphosphate is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). Sdccgsbi-0051172.P002 has been reported in Helianthus tuberosus, Homo sapiens, and other organisms with data available. A uracil nucleotide containing a pyrophosphate group esterified to C5 of the sugar moiety. |
| Molecular Formula |
C9H12N2NA2O12P2
|
|---|---|
| Molecular Weight |
448.12
|
| Exact Mass |
628.029
|
| CAS # |
27821-45-0
|
| Related CAS # |
Uridine 5'-diphosphate sodium salt;21931-53-3
|
| PubChem CID |
6031
|
| Appearance |
White to off-white solid powder
|
| LogP |
-4.7
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
25
|
| Complexity |
676
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1=CN(C(=O)NC1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)O)O)O
|
| InChi Key |
XCCTYIAWTASOJW-XVFCMESISA-N
|
| InChi Code |
InChI=1S/C9H14N2O12P2/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(22-8)3-21-25(19,20)23-24(16,17)18/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,10,12,15)(H2,16,17,18)/t4-,6-,7-,8-/m1/s1
|
| Chemical Name |
[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphono hydrogen phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : 100 mg/mL (223.15 mM)
DMSO : 1 mg/mL (2.23 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (223.15 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2315 mL | 11.1577 mL | 22.3155 mL | |
| 5 mM | 0.4463 mL | 2.2315 mL | 4.4631 mL | |
| 10 mM | 0.2232 mL | 1.1158 mL | 2.2315 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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