| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| Targets |
Tridecanoic acid targets bacterial cells, specifically inhibiting persister cell formation and biofilm formation in Escherichia coli. The compound's mechanism involves disruption of bacterial persistence and biofilm formation, making it effective against antibiotic-resistant bacteria. As a substrate of phospholipase A2, it may also interact with host lipid metabolism pathways.
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|---|---|
| ln Vitro |
Tridecanoic acid demonstrates potent in vitro antibacterial activity. In a direct head-to-head comparison, tridecanoic acid demonstrated the highest antibacterial activity among all saturated fatty acids tested against Streptococcus mutans, with a Minimum Inhibitory Concentration (MIC) of 12.5 μg/ml. It inhibited E. coli BW25113 persister cell formation by 44-fold. Undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli persister cell formation.
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| ln Vivo |
In vivo activity of tridecanoic acid has been suggested by its antibacterial and antibiofilm properties. Its ability to inhibit bacterial persistence and biofilm formation suggests potential for in vivo applications in treating bacterial infections, particularly those associated with biofilms. Further in vivo studies are needed to evaluate its efficacy and safety in animal models.
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| Enzyme Assay |
In vitro enzyme assays for tridecanoic acid are not typically performed, as the compound's mechanism involves disruption of bacterial persistence and biofilm formation rather than specific enzyme inhibition. Its antibacterial activity is assessed using standard microbial growth inhibition assays, where the compound's ability to inhibit bacterial growth is measured and MIC values are determined.
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| Cell Assay |
In vitro cellular assays for tridecanoic acid involve culturing bacterial cells (e.g., E. coli, S. mutans) in the presence of varying concentrations of the compound. Antibacterial activity is assessed by measuring inhibition of growth using optical density measurements or colony counting. Persister cell formation and biofilm formation are assessed using specific assays for persister cell survival and biofilm biomass quantification.
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| Animal Protocol |
In vivo animal experiments for tridecanoic acid are not extensively documented. If used in animal models of bacterial infection, typical protocols would involve administering the compound to infected animals and evaluating efficacy by measuring bacterial load in tissues, biofilm formation, or clinical outcomes. Further studies are needed to characterize its in vivo activity.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for tridecanoic acid are limited. As a medium-chain saturated fatty acid, its absorption, distribution, metabolism, and excretion properties would be influenced by its lipid nature. The compound is found in dairy products and is a product of anaerobic biodegradation. It is a substrate of phospholipase A2. Further pharmacokinetic studies are needed.
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| Toxicity/Toxicokinetics |
Toxicological data for tridecanoic acid are limited. As a naturally occurring fatty acid found in dairy products, it is generally considered to have low toxicity. However, comprehensive toxicological studies have not been reported. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Tridecanoic acid is a C13 straight-chain saturated fatty acid that plays a role in plant metabolism. It is a long-chain fatty acid, a straight-chain saturated fatty acid, and the conjugate acid of Tridecanoic acid. Tridecanoic acid has been reported in Leea guineensis, Inula grandis, and other organisms with relevant data. Tridecanoic acid is a short-chain fatty acid. See also: Fatty acids, C10-16 (note moved here).
Tridecanoic acid (Tridecanoic acid; N-Tridecanoic acid) (CAS#: 638-53-9) has the molecular formula C13H26O2 and a molecular weight of 214.34. It is a 13-carbon medium-chain saturated fatty acid found in dairy products. Tridecanoic acid can serve as an antipersister and antibiofilm agent that may be applied to research bacterial infections. It inhibits E. coli persistence and biofilm formation. It is for research use only. |
| Molecular Formula |
C13H26O2
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|---|---|
| Molecular Weight |
214.34
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| Exact Mass |
214.193
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| CAS # |
638-53-9
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| Related CAS # |
Tridecanoic acid-d2;64118-44-1;Tridecanoic acid-d25;202529-03-1;Tridecanoic acid-d9
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| PubChem CID |
12530
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| Appearance |
White to off-white solid powder
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
308.2±5.0 °C at 760 mmHg
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| Melting Point |
41-42 °C(lit.)
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| Flash Point |
139.6±12.5 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.450
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| LogP |
5.56
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
15
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| Complexity |
144
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SZHOJFHSIKHZHA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H26O2/c1-2-3-4-5-6-7-8-9-10-11-12-13(14)15/h2-12H2,1H3,(H,14,15)
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| Chemical Name |
tridecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (466.55 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 6.25 mg/mL (29.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6655 mL | 23.3274 mL | 46.6548 mL | |
| 5 mM | 0.9331 mL | 4.6655 mL | 9.3310 mL | |
| 10 mM | 0.4665 mL | 2.3327 mL | 4.6655 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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