| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
CECR2 30 nM (IC50) CECR2 10 nM (Kd) CECR2 7.5 (pIC50) BPTF 350 nM (IC50) BPTF 120 nM (Kd) BPTF 6.5 (pIC50) BRD9 5.9 (pIC50)
CECR2 (cat eye syndrome chromosome region candidate 2) and BPTF (bromodomain PHD finger transcription factor). TP-238 is a chemical probe targeting the bromodomains of these proteins, which are involved in chromatin remodeling and gene transcription. |
|---|---|
| ln Vitro |
TP-238 has metabolic activity that is on target at 10–30 nM for CECR2 and 100–350 nM for BPTF. TP-238 shows efficacy in an Alpha screen assay for both BPTF (pIC50 of 6.5) and CECR2 (pIC50 of 7.5). With a Kd of 10 nM for CECR2 and 120 nM for BPTF, isothermal titration calorimetry (ITC) reveals TP-238[1][2].
TP-238 hydrochloride is a potent inhibitor of CECR2 (IC₅0 = 30 nM) and BPTF (IC₅0 = 350 nM). It also inhibits BRD9 with a pIC₅0 of 5.9. It shows minimal activity against 338 other kinases, demonstrating high selectivity. |
| ln Vivo |
No in vivo activity data has been reported for TP-238 hydrochloride in available literature. As a chemical probe, it is primarily used in biochemical and cell-based studies.
|
| Enzyme Assay |
Bromodomain binding assays: TP-238 is incubated with purified bromodomain proteins (CECR2, BPTF, BRD9) in AlphaScreen or TR-FRET assays. Binding affinity is measured by competition with a labeled probe. IC₅0 values are calculated from dose-response curves.
|
| Cell Assay |
Cells are treated with TP-238, and target engagement is assessed by CETSA (cellular thermal shift assay). Chromatin immunoprecipitation (ChIP) can be used to measure the effects on histone modifications and gene transcription. Cell viability is assessed using standard proliferation assays.
|
| Animal Protocol |
No in vivo animal model data has been reported for TP-238 hydrochloride. As a chemical probe for CECR2 and BPTF, it could potentially be used in mouse models to study the role of these bromodomain proteins in disease.
|
| ADME/Pharmacokinetics |
TP-238 hydrochloride has a molecular weight of 495.04 and a molecular formula of C22H31ClN₆O3S. It is a selective dual CECR2/BPTF probe. Detailed pharmacokinetic parameters have not been reported.
|
| Toxicity/Toxicokinetics |
No toxicity data has been published for TP-238 hydrochloride. As a research compound and chemical probe, it is not intended for human therapeutic use. Its selectivity profile suggests low off-target toxicity.
|
| References | |
| Additional Infomation |
TP-238 hydrochloride is a potent and selective dual CECR2/BPTF chemical probe. It inhibits CECR2 (IC₅0 = 30 nM) and BPTF (IC₅0 = 350 nM) with selectivity over 338 other kinases. The compound is for research use only and has not entered clinical trials.
|
| Molecular Formula |
C22H31CLN6O3S
|
|---|---|
| Molecular Weight |
495.04
|
| Exact Mass |
494.186
|
| CAS # |
2415263-05-5
|
| Related CAS # |
TP-238;2415263-04-4
|
| PubChem CID |
146018950
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
33
|
| Complexity |
636
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN(C)CCCOC1=CC=C(C=C1)C2=CC(=NC(=N2)S(=O)(=O)C)NCCCN3C=CC=N3.Cl
|
| InChi Key |
FHVADRKEPFRFFF-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C22H30N6O3S.ClH/c1-27(2)13-6-16-31-19-9-7-18(8-10-19)20-17-21(26-22(25-20)32(3,29)30)23-11-4-14-28-15-5-12-24-28;/h5,7-10,12,15,17H,4,6,11,13-14,16H2,1-3H3,(H,23,25,26);1H
|
| Chemical Name |
6-[4-[3-(dimethylamino)propoxy]phenyl]-2-methylsulfonyl-N-(3-pyrazol-1-ylpropyl)pyrimidin-4-amine;hydrochloride
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 100 mg/mL (202.00 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.05 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.05 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.05 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0200 mL | 10.1002 mL | 20.2004 mL | |
| 5 mM | 0.4040 mL | 2.0200 mL | 4.0401 mL | |
| 10 mM | 0.2020 mL | 1.0100 mL | 2.0200 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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