| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Bacterial riboflavin riboswitches[1]
|
|---|---|
| ln Vitro |
Ribocil-C inhibits the flavin mononucleotide (FMN) riboswitch, which is extremely selective and controls the expression of de novo riboflavin (RF, vitamin B2) production in Escherichia coli. In order to independently regulate the RF production and absorption processes necessary for Staphylococcus aureus growth and pathogenicity, riboflavin-C selectively inhibits dual FMN riboswitches[1]. In order to limit ribB expression and RF production, which in turn stops bacterial growth, Ribocil-C, a synthetic small-molecule FMN mimic, binds the FMN riboswitch of certain GN bacteria, such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii[1][2].
|
| ln Vivo |
In comparison to mice given sham therapy, treatment groups receiving higher doses of Ribocil-C (60 and 120 mg kg21 ribocil-C) show a dose-dependent decrease in bacterial load of 1.87 and 3.29 log10[CFU per g spleen], respectively, without significant toxicity or mortality[2].
|
| References |
| Molecular Formula |
C21H21N7OS
|
|---|---|
| Molecular Weight |
419.502741575241
|
| Exact Mass |
419.152
|
| CAS # |
2309762-18-1
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| Related CAS # |
Ribocil;1381289-58-2;Ribocil-C;1825355-56-3;Ribocil-C (R enantiomer);2177266-81-6
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| PubChem CID |
137220378
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| Appearance |
White to off-white solid powder
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| LogP |
1
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
5
|
| Heavy Atom Count |
30
|
| Complexity |
693
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S1C=CC=C1C1=CC(NC(C2CN(CC3=CN(C4N=CC=CN=4)C=N3)CCC2)=N1)=O
|
| InChi Key |
UVDVCDUBJWYRJW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H21N7OS/c29-19-10-17(18-5-2-9-30-18)25-20(26-19)15-4-1-8-27(11-15)12-16-13-28(14-24-16)21-22-6-3-7-23-21/h2-3,5-7,9-10,13-15H,1,4,8,11-12H2,(H,25,26,29)
|
| Chemical Name |
2-[1-[(1-pyrimidin-2-ylimidazol-4-yl)methyl]piperidin-3-yl]-4-thiophen-2-yl-1H-pyrimidin-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 30 mg/mL (71.51 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3838 mL | 11.9190 mL | 23.8379 mL | |
| 5 mM | 0.4768 mL | 2.3838 mL | 4.7676 mL | |
| 10 mM | 0.2384 mL | 1.1919 mL | 2.3838 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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