| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
N-Hexanoyl-DL-homoserine lactone targets quorum sensing systems in Gram-negative bacteria. It binds to specific receptor proteins in bacteria, leading to the activation or repression of target genes. Its role as a quorum sensing signal in bacteria facilitates communication that enhances biofilm formation and virulence. In plants, it induces transcriptional changes.
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| ln Vitro |
N-Hexanoyl-DL-homoserine lactone demonstrates in vitro activity as a quorum sensing signaling molecule. Application of the compound (60 µM) to tomato leaves suppresses the development of lesions induced by plant pathogenic fungi. It promotes lipid accumulation in algae.
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| ln Vivo |
In vivo activity of N-hexanoyl-DL-homoserine lactone has been demonstrated in plant models. Application to tomato leaves suppresses fungal lesion development. It induces transcriptional changes in Arabidopsis and may contribute to tuning plant growth to the microbial composition of the rhizosphere.
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| Enzyme Assay |
In vitro receptor binding assays for N-hexanoyl-DL-homoserine lactone involve measuring its binding to quorum sensing receptors. The compound's ability to activate quorum sensing pathways can be evaluated using reporter gene assays. Its effects on plants can be assessed by measuring transcriptional changes in Arabidopsis.
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| Cell Assay |
In vitro cellular assays for N-hexanoyl-DL-homoserine lactone involve treating bacterial cultures with varying concentrations of the compound and measuring quorum-sensing-regulated phenotypes such as biofilm formation and virulence factor production. Plant assays involve applying the compound to leaves and measuring lesion development.
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| Animal Protocol |
In vivo animal experiments for N-hexanoyl-DL-homoserine lactone are not extensively documented. The compound is primarily studied in plant models for its effects on plant-microbe interactions. Further studies in animal models of bacterial infection could evaluate its role in pathogenesis.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for N-hexanoyl-DL-homoserine lactone are limited. The compound has a molecular weight of 199.25 and a molecular formula of C10H17NO3. It is a bacterial quorum sensing molecule produced in the rhizosphere. Its stability and bioavailability in biological systems require further investigation.
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| Toxicity/Toxicokinetics |
Toxicological data for N-hexanoyl-DL-homoserine lactone are limited. As a bacterial signaling molecule, it is generally considered to have low toxicity in research settings. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
[1]. von Rad U, et al. Response of Arabidopsis thaliana to N-hexanoyl-DL-homoserine-lactone, a bacterial quorum sensing molecule produced in the rhizosphere. Planta. 2008;229(1):73-85.
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| Additional Infomation |
N-(2-oxooxopentane-3-yl)hexylamide is an N-acyl amino acid. It has been reported that N-(2-oxooxopentane-3-yl)hexylamide has been found in Vibrio fischeri, and relevant data are available for reference.
N-Hexanoyl-DL-homoserine lactone (N-Hexanoyl-DL-homoserine lactone) (CAS#: 106983-28-2) has the molecular formula C10H17NO3 and a molecular weight of 199.25. It is a member of the N-acyl-homoserine lactone family involved in quorum sensing in Gram-negative bacteria. The compound induces transcriptional changes in plants and suppresses fungal lesion development. |
| Molecular Formula |
C10H17NO3
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|---|---|
| Molecular Weight |
199.25
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| Exact Mass |
199.121
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| CAS # |
106983-28-2
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| PubChem CID |
3462373
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| Appearance |
White to off-white solid powder
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| Melting Point |
101-106 °C
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| LogP |
1.838
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
14
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| Complexity |
215
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCC(NC1CCOC1=O)=O
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| InChi Key |
ZJFKKPDLNLCPNP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H17NO3/c1-2-3-4-5-9(12)11-8-6-7-14-10(8)13/h8H,2-7H2,1H3,(H,11,12)
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| Chemical Name |
N-(2-oxooxolan-3-yl)hexanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (501.88 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.55 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.55 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0188 mL | 25.0941 mL | 50.1882 mL | |
| 5 mM | 1.0038 mL | 5.0188 mL | 10.0376 mL | |
| 10 mM | 0.5019 mL | 2.5094 mL | 5.0188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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