| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Quorum sensing receptors in Gram-negative bacteria (e.g., LuxR-type receptors). C10-HSL is a natural AHL signal molecule that binds to quorum sensing receptors and activates downstream gene expression. The specific receptor depends on the bacterial species, but C10-HSL generally activates LuxR-type transcriptional regulators that control virulence, biofilm formation, and other QS-regulated phenotypes.
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| ln Vitro |
In Arabidopsis, N-decanoyl-L-homoserine lactone (0-75 µM) reduces primary root growth and encourages the production of lateral roots [1]. In wild-type seedlings, N-decanoyl-L-homoserine lactone (0–30µM) can raise the cytosolic Ca2+ concentration [1]. Through cross-kingdom interactions, the bacterial signal N-decanoyl-L-homoserine lactone promotes the germination of Galactomyces geotrichum spores [2].
In vitro, C10-HSL acts as a quorum sensing signal molecule in various Gram-negative bacterial species. It regulates quorum sensing-dependent gene expression, including genes involved in biofilm formation, virulence factor production, and bioluminescence (in reporter strains). The compound is used as a standard in analytical assays and as a signaling molecule to study QS mechanisms and host-pathogen interactions. |
| ln Vivo |
In vivo activity data for C10-HSL are limited. As a bacterial signaling molecule, it is typically studied in vitro or in ex vivo models rather than in animal models. The compound's role in bacterial pathogenesis and host interactions can be studied using infection models where QS is known to play a role. However, C10-HSL itself is not used as a therapeutic agent but as a research tool.
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| Enzyme Assay |
Quorum sensing activation is assessed using reporter strains (e.g., E. coli or Agrobacterium tumefaciens harboring QS-responsive reporter plasmids). Bacteria are cultured in the presence of varying concentrations of C10-HSL, and reporter gene expression (e.g., β-galactosidase, luciferase, or GFP) is measured. The EC50 for QS activation is determined from dose-response curves. Analytical quantification is performed by LC-MS/MS.
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| Cell Assay |
C10-HSL is used in cell-based (bacterial) assays to study quorum sensing regulation. Bacteria (e.g., Pseudomonas aeruginosa, Vibrio fischeri, or reporter strains) are cultured in the presence of C10-HSL at various concentrations. QS-regulated phenotypes (e.g., biofilm formation, pigment production, virulence factor secretion) are quantified. Gene expression is analyzed by qRT-PCR or reporter assays.
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| Animal Protocol |
C10-HSL is not typically used in animal models as a therapeutic agent. However, it may be used in infection models to study the role of quorum sensing in bacterial pathogenesis. For example, mice may be infected with QS-deficient bacterial strains, and C10-HSL may be administered to complement the QS deficiency and restore virulence. Such studies help elucidate the role of QS in infection.
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| ADME/Pharmacokinetics |
C10-HSL has molecular formula C14H25NO3 and molecular weight 255.35. It is also known as N-decanoyl-L-homoserine lactone. CAS number is 177315-87-6. The compound is a quorum sensing signaling molecule used as a standard and research tool. Its pharmacokinetic properties are not relevant for its use as a research standard. Storage at -20°C is recommended.
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| Toxicity/Toxicokinetics |
No toxicity data are specifically reported for C10-HSL. As a bacterial signaling molecule, it is not used as a therapeutic agent and its safety profile is not relevant for clinical applications. Standard laboratory safety precautions should be followed when handling the compound. It is for research use only and is not intended for human or veterinary use.
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| References |
[1]. Cao XY, et al. Cellular messengers involved in the inhibition of the Arabidopsis primary root growth by bacterial quorum-sensing signal N-decanoyl-L-homoserine lactone. BMC Plant Biol. 2022 Oct 14;22(1):488.
[2]. Xin Lu, et al. Bacterial signal C10-HSL stimulates spore germination of Galactomyces geotrichum by transboundary interaction. Chinese Chemical Letters. 2022. |
| Additional Infomation |
N-decanoyl-L-homoserine lactone is an N-acyl amino acid. It has been reported that N-[(3s)-2-oxotetrahydrofuran-3-yl]decanoamide has been found in Azotobacter lipodilator, and relevant data are available for reference.
N-Decanoyl-L-homoserine lactone (C10-HSL) is a naturally occurring N-acyl homoserine lactone (AHL) quorum sensing signal molecule in Gram-negative bacteria. It activates LuxR-type quorum sensing receptors and regulates QS-dependent gene expression. The compound is used as a standard and signaling molecule in quorum sensing research, biofilm studies, and host-pathogen interaction studies. It has not entered clinical trials or received FDA approval. Purity is typically ≥95-98%. |
| Molecular Formula |
C14H25NO3
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|---|---|
| Molecular Weight |
255.35
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| Exact Mass |
255.183
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| CAS # |
177315-87-6
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| PubChem CID |
10131281
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
466.1±34.0 °C at 760 mmHg
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| Melting Point |
127-130 °C (lit.)
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| Flash Point |
235.7±25.7 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.476
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| LogP |
2.09
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
18
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| Complexity |
266
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| Defined Atom Stereocenter Count |
1
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| SMILES |
O1C([C@]([H])(C([H])([H])C1([H])[H])N([H])C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])=O)=O
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| InChi Key |
TZWZKDULKILUPV-LBPRGKRZSA-N
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| InChi Code |
InChI=1S/C14H25NO3/c1-2-3-4-5-6-7-8-9-13(16)15-12-10-11-18-14(12)17/h12H,2-11H2,1H3,(H,15,16)/t12-/m0/s1
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| Chemical Name |
N-[(3S)-2-oxooxolan-3-yl]decanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (391.62 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.79 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (9.79 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9162 mL | 19.5810 mL | 39.1619 mL | |
| 5 mM | 0.7832 mL | 3.9162 mL | 7.8324 mL | |
| 10 mM | 0.3916 mL | 1.9581 mL | 3.9162 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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