| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
| Targets |
Methyl benzethonium chloride targets microbial cell membranes. As a quaternary ammonium compound, it acts as a cationic surfactant that disrupts the cell membrane of microorganisms, leading to cell lysis and death. Its broad-spectrum antimicrobial activity makes it effective against a wide range of pathogens.
|
|---|---|
| ln Vitro |
Benzethonium chloride is a biochemical reagent that can be utilized in life science research as an organic compound or biological material.
In vitro, methyl benzethonium chloride exhibits broad-spectrum antimicrobial activity. It is effective against bacteria, viruses, and fungi. Its activity is typically assessed using standard antimicrobial susceptibility tests, such as the broth microdilution method, to determine its minimum inhibitory concentration (MIC) against a panel of microorganisms. |
| ln Vivo |
In vivo, methyl benzethonium chloride is used as a preservative and disinfectant in various products. It has been used in a study to investigate the anti-leishmanial activity of paromomycin ointment combined with the immunomodulator Imiquimod. Its use in these applications is based on its broad-spectrum antimicrobial properties.
|
| Enzyme Assay |
The antimicrobial activity of methyl benzethonium chloride is assessed using standard in vitro susceptibility tests. Bacterial or fungal cultures are incubated with varying concentrations of the compound, and the minimum inhibitory concentration (MIC) is determined. Its activity against viruses can be assessed using viral plaque assays.
|
| Cell Assay |
The cellular activity of methyl benzethonium chloride is evaluated in cell culture models to study its effects on mammalian cells. It has been used in a study to assess stem cell death-inducing small molecules. Its effect on cell viability and proliferation can be measured using standard assays.
|
| Animal Protocol |
In animal studies, methyl benzethonium chloride is not typically administered systemically. Its use is primarily as a topical agent or preservative. Its safety and efficacy as a preservative and disinfectant have been established through its long history of use in various products.
|
| ADME/Pharmacokinetics |
Methyl benzethonium chloride has a molecular weight of 462.11 g/mol and a chemical formula of C28H44ClNO2. It is a quaternary ammonium compound that is soluble in water and organic solvents. It is typically stored as a powder at room temperature. Its stability and purity are important for its use as a preservative.
|
| Toxicity/Toxicokinetics |
The toxicity profile of methyl benzethonium chloride is related to its antimicrobial activity. As a cationic surfactant, it can be irritating to skin and mucous membranes. Its safety as a preservative in personal care products has been established through regulatory review.
|
| Additional Infomation |
Methylbenzethonium chloride is an alkylbenzene.
Methyl benzethonium chloride (CAS: 25155-18-4) is a widely used quaternary ammonium compound with broad-spectrum antimicrobial properties. Its use as a preservative and disinfectant in personal care and wellness products highlights its importance in maintaining product safety and stability. It is also used as a research tool in studies of antimicrobial activity and cell death. |
| Molecular Formula |
C28H45CLNO2
|
|---|---|
| Molecular Weight |
463.12
|
| Exact Mass |
461.306
|
| CAS # |
25155-18-4
|
| PubChem CID |
5702238
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
159-162 °C
|
| LogP |
3.384
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
32
|
| Complexity |
502
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1=C(C=CC(=C1)C(C)(C)CC(C)(C)C)OCCOCC[N+](C)(C)CC2=CC=CC=C2.[Cl-]
|
| InChi Key |
QWZLBLDNRUUYQI-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C28H44NO2.ClH/c1-23-20-25(28(5,6)22-27(2,3)4)14-15-26(23)31-19-18-30-17-16-29(7,8)21-24-12-10-9-11-13-24;/h9-15,20H,16-19,21-22H2,1-8H3;1H/q+1;/p-1
|
| Chemical Name |
benzyl-dimethyl-[2-[2-[2-methyl-4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethyl]azanium;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~54.10 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (2.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1593 mL | 10.7963 mL | 21.5927 mL | |
| 5 mM | 0.4319 mL | 2.1593 mL | 4.3185 mL | |
| 10 mM | 0.2159 mL | 1.0796 mL | 2.1593 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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