| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
AMPK
Metformin-d6 hydrochloride does not have a distinct biological target as it is an analytical internal standard. The parent compound, metformin hydrochloride, targets the mitochondrial respiratory chain in the liver, leading to AMPK activation and enhanced insulin sensitivity. Metformin suppresses glucose production by the liver and is used to treat type 2 diabetes. |
|---|---|
| ln Vitro |
Metformin-d6 hydrochloride does not exhibit pharmacological activity as it is used exclusively as an analytical standard. Its utility is in enabling accurate quantification of metformin in biological samples by mass spectrometry. The compound exhibits identical chromatographic and mass spectrometric behavior to metformin, allowing for precise analytical measurements.
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| ln Vivo |
Metformin-d6 hydrochloride is not used as a therapeutic agent and does not exhibit in vivo activity. It is administered as an internal standard in pharmacokinetic studies to accurately quantify metformin concentrations. The parent compound, metformin, can penetrate the blood-brain barrier and induce autophagy.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not performed for metformin-d6 hydrochloride, as it is an analytical standard. Its characterization involves analytical method validation, including assessment of its chromatographic behavior and mass spectrometric response. The compound is used as a reference standard for the detection and quantification of metformin in biological samples.
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| Cell Assay |
In vitro cellular assays are not applicable to metformin-d6 hydrochloride, as it is an analytical standard used for quantification purposes. The compound is spiked into biological samples to serve as an internal standard for analytical methods such as LC-MS/MS or GC-MS.
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| Animal Protocol |
In vivo animal experiments for metformin-d6 hydrochloride involve administering the compound alongside metformin in pharmacokinetic studies. Blood samples are collected at various time points, and metformin concentrations are measured by mass spectrometry using the deuterated internal standard for normalization.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for metformin-d6 hydrochloride are not separately characterized, as it is used as an internal standard for measuring metformin levels. The compound has a molecular weight of 171.66 and a molecular formula of C4H6D6ClN5. Purity is typically >98% with >98% atom% D. Stability is ensured if stored under recommended conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for metformin-d6 hydrochloride are not separately evaluated, as it is used in trace amounts as an analytical internal standard. The compound is for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Metformin-d6 hydrochloride (1,1-Dimethylbiguanide-d6 hydrochloride) (CAS#: 1185166-01-1) has the molecular formula C4H6D6ClN5 and a molecular weight of 171.66. It is a deuterium-labeled internal standard for metformin quantification by mass spectrometry. The parent compound, metformin hydrochloride, inhibits the mitochondrial respiratory chain, leading to AMPK activation. Purity is >98%. It is for research use only.
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| Molecular Formula |
C4H6D6CLN5
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|---|---|
| Molecular Weight |
171.66
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| Exact Mass |
171.115
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| CAS # |
1185166-01-1
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| Related CAS # |
Metformin hydrochloride;1115-70-4
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| PubChem CID |
45039707
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| Appearance |
White to off-white solid powder
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| Melting Point |
215-218°C
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| LogP |
1.058
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
10
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| Complexity |
132
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])N(C(=N)N=C(N)N)C([2H])([2H])[2H].Cl
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| InChi Key |
OETHQSJEHLVLGH-TXHXQZCNSA-N
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| InChi Code |
InChI=1S/C4H11N5.ClH/c1-9(2)4(7)8-3(5)6;/h1-2H3,(H5,5,6,7,8);1H/i1D3,2D3;
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| Chemical Name |
3-(diaminomethylidene)-1,1-bis(trideuteriomethyl)guanidine;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 50 mg/mL (291.27 mM)
DMSO : 50 mg/mL (291.27 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (12.12 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (12.12 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8255 mL | 29.1273 mL | 58.2547 mL | |
| 5 mM | 1.1651 mL | 5.8255 mL | 11.6509 mL | |
| 10 mM | 0.5825 mL | 2.9127 mL | 5.8255 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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