| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
L-Glutamic acid-d5 itself does not have a traditional biological target; it is a stable isotope-labeled internal standard. The parent compound L-Glutamic acid is a non-essential amino acid that is the most abundant excitatory neurotransmitter in the vertebrate nervous system. It is involved in numerous metabolic processes and serves as a precursor for the synthesis of other amino acids and neurotransmitters. L-Glutamic acid-d5 is used to track and quantify L-Glutamic acid in biological samples for metabolic studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
L-Glutamic acid-d5 does not exhibit direct in vitro biological activity as it is an analytical standard. However, the parent compound L-Glutamic acid is a non-essential amino acid and the most abundant excitatory neurotransmitter in the vertebrate nervous system. L-Glutamic acid-d5 is used as an internal standard to accurately measure L-Glutamic acid concentrations in biological samples, facilitating research on amino acid metabolism and neurotransmission. |
| ln Vivo |
L-Glutamic acid-d5 is not intended for in vivo administration as a therapeutic agent; it is used as an analytical standard. The in vivo activity of the parent compound L-Glutamic acid is well documented as a neurotransmitter and metabolic intermediate. L-Glutamic acid-d5 is utilized in pharmacokinetic and metabolic studies to track the distribution, metabolism, and excretion of L-Glutamic acid in biological systems.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for L-Glutamic acid-d5 are not typically performed, as it is an analytical standard. Instead, the compound is used in analytical method development and validation. Quality control assays involve HPLC or LC-MS/MS to verify isotopic purity (≥98 atom % D) and chemical purity. The compound is tested for stability under various storage conditions. It appears as a solid and is soluble in water.
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| Cell Assay |
L-Glutamic acid-d5 itself is not tested in in vitro cellular assays for biological activity, as it is an analytical standard. Its utility is assessed by its performance as an internal standard in analytical workflows, including its ability to provide accurate quantification in LC-MS/MS analyses. The compound is used in quality control applications for L-Glutamic acid analysis.
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| Animal Protocol |
In vivo animal studies for L-Glutamic acid-d5 are not conducted as therapeutic efficacy studies. Instead, the compound is administered to animals as a tracer in pharmacokinetic studies to quantify the parent compound L-Glutamic acid in plasma, tissues, and excreta. These studies help determine the absorption, distribution, metabolism, and excretion (ADME) profile of L-Glutamic acid.
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| ADME/Pharmacokinetics |
L-Glutamic acid-d5 has a molecular weight of approximately 152.16 and a molecular formula of C5H4D5NO4. It has a purity of ≥98% and an isotopic enrichment of ≥98 atom % D. It appears as a solid. The compound is stable under recommended storage conditions. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of L-Glutamic acid-d5 is not extensively characterized, as it is an analytical standard rather than a therapeutic agent. The parent compound L-Glutamic acid is a naturally occurring amino acid that is generally recognized as safe. L-Glutamic acid-d5 is intended for research use only and is not approved for clinical use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
L-glutamic acid-d5 is a deuterated compound of L-glutamic acid.
L-Glutamic acid-d5 (CAS 2784-50-1) is the deuterium-labeled form of L-Glutamic acid. L-Glutamic acid is a non-essential amino acid that is the most abundant excitatory neurotransmitter in the vertebrate nervous system. It is involved in numerous metabolic processes and serves as a precursor for the synthesis of other amino acids and neurotransmitters. L-Glutamic acid-d5 is used as an internal standard for the quantification of L-Glutamic acid by GC- or LC-MS. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C5H4D5NO4
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|---|---|
| Molecular Weight |
152.16
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| Exact Mass |
152.084
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| CAS # |
2784-50-1
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| Related CAS # |
L-Glutamic acid;56-86-0
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| PubChem CID |
12248503
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
333.8±32.0 °C at 760 mmHg
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| Melting Point |
205ºC (dec.)(lit.)
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| Flash Point |
155.7±25.1 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.522
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| LogP |
-1.43
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
145
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H][C@@](C(=O)O)(C([2H])([2H])C([2H])([2H])C(=O)O)N
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| InChi Key |
WHUUTDBJXJRKMK-NKXUJHECSA-N
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| InChi Code |
InChI=1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m0/s1/i1D2,2D2,3D
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| Chemical Name |
(2S)-2-amino-2,3,3,4,4-pentadeuteriopentanedioic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 14.29 mg/mL (93.91 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 7.14 mg/mL (46.92 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5720 mL | 32.8601 mL | 65.7203 mL | |
| 5 mM | 1.3144 mL | 6.5720 mL | 13.1441 mL | |
| 10 mM | 0.6572 mL | 3.2860 mL | 6.5720 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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