| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
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| 5mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
Bacterial and mammalian nucleic acids (DNA). Hexidium iodide is a cationic dye that binds to nucleic acids, emitting bright fluorescence upon binding. It allows for differentiation between Gram-positive and Gram-negative bacteria based on differential absorption through bacterial cell walls.
|
|---|---|
| ln Vitro |
Hexidium iodide selectively stains gram-positive organisms in suspension, enabling the determination of Gram status through differential absorption through bacterial cell walls[1].
Hexidium iodide functions as a fluorescent nucleic acid stain. It selectively stains almost all Gram-positive bacteria in the presence of Gram-negative bacteria. Upon binding DNA, it exhibits excitation/emission at approximately 518/600 nm. It can penetrate mammalian cells. |
| ln Vivo |
No in vivo activity as a therapeutic agent; used as a diagnostic or research staining tool. It can be used to assess Gram status in bacterial samples and for fluorescence-based bacterial detection.
|
| Enzyme Assay |
Hexidium iodide is used in fluorescence microscopy, flow cytometry, and fluorometric assays for bacterial detection and differentiation. The dye is added to bacterial suspensions, and fluorescence is measured at excitation/emission wavelengths of ~518/600 nm. Staining specificity for Gram-positive bacteria is confirmed by comparison with Gram-negative controls.
|
| Cell Assay |
Bacterial cultures (Gram-positive and Gram-negative strains) are incubated with Hexidium iodide. Fluorescence is measured using flow cytometry or fluorescence microscopy. Staining patterns are compared to determine the ability of the dye to differentiate between bacterial types. The dye can also be used to stain mammalian cells for nucleic acid detection.
|
| Animal Protocol |
Animal models of bacterial infection could potentially be used to evaluate Hexidium iodide as a diagnostic tool for detecting Gram-positive bacteria in vivo. However, it is primarily used as an in vitro research reagent.
|
| ADME/Pharmacokinetics |
Hexidium iodide has a molecular weight of 497.41 and a molecular formula of C2₅H2₈IN3. It is a fluorescent nucleic acid stain with excitation/emission at ~518/600 nm. Storage: -20degC, protect from light.
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| Toxicity/Toxicokinetics |
No toxicity data has been published for Hexidium iodide. As a fluorescent stain and research reagent, it is not intended for human therapeutic use. Standard laboratory safety precautions should be followed.
|
| References | |
| Additional Infomation |
Hexidium iodide is a fluorescent nucleic acid stain that selectively stains almost all Gram-positive bacteria. It has excitation/emission at ~518/600 nm and can penetrate mammalian cells. It is used for bacterial detection and differentiation. The compound is for research use only.
|
| Molecular Formula |
C25H28IN3
|
|---|---|
| Molecular Weight |
497.41
|
| Exact Mass |
497.133
|
| CAS # |
211566-66-4
|
| PubChem CID |
71358343
|
| Appearance |
Brown to reddish brown solid powder
|
| LogP |
7.782
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
29
|
| Complexity |
474
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCCCCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[I-]
|
| InChi Key |
DBMJYWPMRSOUGB-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C25H27N3.HI/c1-2-3-4-8-15-28-24-17-20(27)12-14-22(24)21-13-11-19(26)16-23(21)25(28)18-9-6-5-7-10-18;/h5-7,9-14,16-17,27H,2-4,8,15,26H2,1H3;1H
|
| Chemical Name |
5-hexyl-6-phenylphenanthridin-5-ium-3,8-diamine;iodide
|
| Synonyms |
Hexidium iodide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~100.5 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0104 mL | 10.0521 mL | 20.1041 mL | |
| 5 mM | 0.4021 mL | 2.0104 mL | 4.0208 mL | |
| 10 mM | 0.2010 mL | 1.0052 mL | 2.0104 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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