| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
Ethidium Bromide (EtBr) is occasionally added to the running buffer when using agarose gel electrophoresis to separate DNA fragments. It is utilized because the DNA banding pattern can be seen when the molecule binds to the DNA and is illuminated with a UV light source. Intercalation between base pairs is how EtBr binds. The DNA molecule's weight, charge, conformation, and flexibility are all altered by this binding. DNA has always moved less freely in the gels containing EtBr[1].
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| References |
| Molecular Formula |
C21H20BRN3
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|---|---|
| Molecular Weight |
394.31
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| Exact Mass |
393.08
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| Elemental Analysis |
C, 63.97; H, 5.11; Br, 20.26; N, 10.66
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| CAS # |
1239-45-8
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| Related CAS # |
1239-45-8 (Br); 3546-21-2; 602-52-8 (Cl)
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| Appearance |
Powder
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| SMILES |
CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
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| InChi Key |
ZMMJGEGLRURXTF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
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| Chemical Name |
5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine;bromide
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| Synonyms |
Dromilac; ETHIDIUM BROMIDE; EtBr; Homidium bromide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~78 mg/mL (~197.8 mM)
Ethanol : ~1 mg/mL (~2.5 mM) Water : ~7 mg/mL (~17.8 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 9.09 mg/mL (23.05 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5361 mL | 12.6804 mL | 25.3608 mL | |
| 5 mM | 0.5072 mL | 2.5361 mL | 5.0722 mL | |
| 10 mM | 0.2536 mL | 1.2680 mL | 2.5361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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