| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
Delphinidin-3-O-galactoside chloride targets various cellular pathways through its antioxidant and anti-inflammatory mechanisms. As an anthocyanin, it scavenges reactive oxygen species (ROS) and modulates signaling pathways involved in inflammation and cell survival. The compound may interact with nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPKs), and other signaling molecules to exert its biological effects. The specific molecular targets are still being elucidated through ongoing research.
|
|---|---|
| ln Vitro |
In vitro studies have demonstrated that Delphinidin-3-O-galactoside chloride exhibits potent antioxidant activity through free radical scavenging and metal chelation. It has been shown to inhibit inflammatory cytokine production and modulate cellular signaling pathways. The compound's activity is typically assessed using standard antioxidant assays such as DPPH, ABTS, and FRAP, as well as cell-based assays measuring oxidative stress markers and inflammatory responses.
|
| ln Vivo |
In vivo studies for Delphinidin-3-O-galactoside chloride have been limited, though anthocyanins in general have shown health benefits in animal models and human studies. As a dietary anthocyanin, it may contribute to the health benefits associated with consumption of colorful fruits and vegetables. Studies in appropriate animal models would be needed to evaluate its specific in vivo efficacy, pharmacokinetics, and safety profile for therapeutic applications.
|
| Enzyme Assay |
For antioxidant activity assays, Delphinidin-3-O-galactoside chloride is tested using standard methods such as DPPH radical scavenging, ABTS radical cation decolorization, and FRAP (ferric reducing antioxidant power) assays. The compound is dissolved in appropriate solvents and tested at various concentrations. The percentage of radical scavenging or reducing power is calculated and compared to standard antioxidants such as Trolox or ascorbic acid. IC50 values are determined from dose-response curves.
|
| Cell Assay |
For cellular studies, appropriate cell lines (e.g., endothelial cells, immune cells, or cancer cells) are cultured in appropriate media and treated with Delphinidin-3-O-galactoside chloride at various concentrations (typically 1-100 μM). Cellular antioxidant activity is assessed by measuring ROS levels using fluorescent probes such as DCFH-DA. Anti-inflammatory activity is evaluated by measuring cytokine production (TNF-α, IL-6, IL-1β) by ELISA. Cell viability is assessed using MTT assays to determine the compound's cytotoxicity profile.
|
| Animal Protocol |
For in vivo studies, appropriate animal models would be used to evaluate Delphinidin-3-O-galactoside chloride. The compound would be administered via oral gavage or intraperitoneal injection at various doses. For antioxidant studies, biomarkers of oxidative stress in plasma and tissues would be measured. For anti-inflammatory studies, inflammatory markers and tissue histopathology would be assessed. However, detailed in vivo protocols for this specific compound have not been extensively reported.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Delphinidin-3-O-galactoside chloride have not been fully characterized in the available literature. As an anthocyanin glycoside, it is expected to have limited oral bioavailability due to poor absorption and extensive metabolism. The compound is typically stored at -20°C to maintain stability. Comprehensive PK studies are needed to fully understand its absorption, distribution, metabolism, and excretion profile.
|
| Toxicity/Toxicokinetics |
Delphinidin-3-O-galactoside chloride is a research compound intended for laboratory use only and is not approved for human therapeutic use. As a naturally occurring anthocyanin, it is used in research for studying antioxidant, anti-inflammatory, and health-promoting properties. Standard laboratory safety precautions should be followed when handling this compound. Storage at -20°C is recommended.
|
| References | |
| Additional Infomation |
Delphinidin-3-O-galactoside chloride is a naturally occurring anthocyanin pigment (delphinidin-3-galactoside chloride) with potent antioxidant properties. It is found in various fruits and flowers and is used in research for studying the health benefits of anthocyanins. The compound is for research use only and has not been approved for clinical applications.
|
| Molecular Formula |
C21H21CLO12
|
|---|---|
| Molecular Weight |
500.84
|
| Exact Mass |
500.072
|
| CAS # |
28500-00-7
|
| PubChem CID |
176440
|
| Appearance |
Brown to dark brown solid powder
|
| Hydrogen Bond Donor Count |
9
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
34
|
| Complexity |
641
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C1=C(C=C(C(=C1O)O)O)C2=[O+]C3=CC(=CC(=C3C=C2O[C@H]4[C@@H]([C@H]([C@H]([C@H](O4)CO)O)O)O)O)O.[Cl-]
|
| InChi Key |
ZJWIIMLSNZOCBP-KGDMUXNNSA-N
|
| InChi Code |
InChI=1S/C21H20O12.ClH/c22-6-15-17(28)18(29)19(30)21(33-15)32-14-5-9-10(24)3-8(23)4-13(9)31-20(14)7-1-11(25)16(27)12(26)2-7;/h1-5,15,17-19,21-22,28-30H,6H2,(H4-,23,24,25,26,27);1H/t15-,17+,18+,19-,21-;/m1./s1
|
| Chemical Name |
(2S,3R,4S,5R,6R)-2-[5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)chromenylium-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 100 mg/mL (199.66 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9966 mL | 9.9832 mL | 19.9665 mL | |
| 5 mM | 0.3993 mL | 1.9966 mL | 3.9933 mL | |
| 10 mM | 0.1997 mL | 0.9983 mL | 1.9966 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
