| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
CTP targets RNA polymerases, serving as a substrate for RNA synthesis. During transcription, RNA polymerase incorporates CTP into the growing RNA chain by forming a phosphodiester bond between the 3' hydroxyl group of the ribose sugar in the existing RNA chain and the α-phosphate of CTP. CTP also acts as a substrate for CTP:phosphocholine cytidylyltransferase in the synthesis of phosphatidylcholine. |
|---|---|
| ln Vitro |
CTP demonstrates in vitro activity as a substrate for RNA polymerases in transcription reactions. A 31-fold difference in Km indicates that native CTP binds with substantially higher efficiency to RNA polymerase, making it the required substrate. The compound's activity is assessed by its ability to support RNA synthesis in vitro, where it is incorporated into RNA transcripts opposite guanine residues in the template strand.
|
| ln Vivo |
CTP is essential for RNA synthesis in vivo and is involved in the synthesis of mRNA, rRNA, and tRNA through RNA polymerases. It also plays a critical role in the synthesis of phosphatidylcholine via the enzyme CTP:phosphocholine cytidyltransferase. As an endogenous metabolite, CTP is present in all living organisms and participates in numerous biochemical pathways.
|
| Enzyme Assay |
In vitro enzyme assays for CTP involve measuring its incorporation into RNA by RNA polymerases in transcription reactions. The assay typically uses purified RNA polymerase, a DNA template, and a mixture of NTPs including radiolabeled or fluorescently labeled CTP. CTP:phosphocholine cytidylyltransferase activity can be measured by detecting the formation of CDP-choline from CTP and phosphocholine.
|
| Cell Assay |
In vitro cellular assays for CTP are not typically performed, as it is a naturally occurring nucleotide used as a substrate in biochemical reactions. Its role in RNA synthesis can be studied in cell-free transcription systems or in cell culture models by measuring RNA production or by using labeled CTP to track RNA synthesis.
|
| Animal Protocol |
CTP is not typically administered in animal experiments as a therapeutic agent. It is a naturally occurring nucleotide that is essential for RNA synthesis in all living organisms. Its role in transcription makes it essential for gene expression and cellular function. In research settings, CTP may be used in cell-free transcription systems.
|
| ADME/Pharmacokinetics |
CTP is a naturally occurring nucleotide that is synthesized intracellularly. Its concentration is tightly regulated within cells. CTP is involved in RNA synthesis, phospholipid synthesis, and protein glycosylation. The compound is used as a research reagent in molecular biology and biochemistry applications. It is supplied as a sodium salt hydrate for enhanced stability.
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| Toxicity/Toxicokinetics |
CTP is a naturally occurring nucleotide and is not considered toxic at physiological concentrations. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
CTP stands for pyrimidine ribonucleoside 5'-triphosphate and cytidine 5'-phosphate. It is a metabolite in both E. coli and mice. It is the conjugate acid of CTP(4-) and CTP(3-). Cytidine triphosphate is a metabolite found or produced in E. coli (K12 strain, MG1655 strain). It has been reported to be detected in Homo sapiens, Apis cerana, and Caenorhabditis elegans, with relevant data available. Cytidine triphosphate is a cytosine-derived nucleotide composed of three phosphate groups esterified onto a deoxyribose glycosyl group. Nucleotide metabolism is upregulated in cancer cells; therefore, this nucleotide can be used as a biomarker for detecting the presence of cancer cells. Cytidine triphosphate is a metabolite found or produced in Saccharomyces cerevisiae. Cytidine 5'-(tetrahydrotriphosphate). A cytosine nucleotide whose glycosyl moiety is esterified with three phosphate groups.
Cytidine-5'-triphosphate (CTP) (CAS#: 65-47-4) has the molecular formula C9H16N3O14P3 and a molecular weight of 483.16. It is a pyrimidine nucleoside triphosphate and a high-energy molecule that serves as a substrate for RNA synthesis by RNA polymerases. CTP is also critical for the synthesis of phosphatidylcholine and other membrane phospholipids. |
| Molecular Formula |
C9H16N3O14P3
|
|---|---|
| Molecular Weight |
483.16
|
| Exact Mass |
482.984
|
| CAS # |
65-47-4
|
| Related CAS # |
Cytidine-5'-triphosphate disodium;36051-68-0;Cytidine-5′-triphosphate-d14 disodium
|
| PubChem CID |
6176
|
| Appearance |
White to off-white solid powder
|
| Density |
2.5±0.1 g/cm3
|
| Boiling Point |
849.2±75.0 °C at 760 mmHg
|
| Melting Point |
215 - 218 °C
|
| Flash Point |
467.4±37.1 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.801
|
| LogP |
-5.52
|
| Hydrogen Bond Donor Count |
7
|
| Hydrogen Bond Acceptor Count |
14
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
29
|
| Complexity |
855
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O
|
| InChi Key |
PCDQPRRSZKQHHS-XVFCMESISA-N
|
| InChi Code |
InChI=1S/C9H16N3O14P3/c10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H2,10,11,15)(H2,16,17,18)/t4-,6-,7-,8-/m1/s1
|
| Chemical Name |
[[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : 83.33 mg/mL (172.47 mM)
DMSO : < 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (206.97 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0697 mL | 10.3485 mL | 20.6971 mL | |
| 5 mM | 0.4139 mL | 2.0697 mL | 4.1394 mL | |
| 10 mM | 0.2070 mL | 1.0349 mL | 2.0697 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Link: https://clinicaltrials.gov/ct2/show/NCT03505684
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