Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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Targets |
Kd: 3 nM (Calmodulin)[3]
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ln Vitro |
In addition to its common usage as a calmodulin (CaM) antagonist, carbamazole is also known to cause apoptosis in specific cancer cell lines. Mouse F9 ECC development is inhibited by trimetazolium chloride (3, 5, 7, 10 μM, 30 minutes-24 hours) [2].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: Mouse F9 ECCs Tested Concentrations: 3, 5, 7, 10 μM Incubation Duration: 30 minutes-24 hrs (hours) Experimental Results: The IC50s of Calmidazolium chloride treated F9 ECCs and E14 ESCs are 8.18 μM, and 12.69 μM[2]. |
References |
[1]. Gietzen K, et al. Comparison of the calmodulin antagonists compound 48/80 and calmidazolium. Biochem J. 1983 Dec 15;216(3):611-6.
[2]. Lee J, et al. Calmidazolium chloride inhibits growth of murine embryonal carcinoma cells, a model of cancer stem-like cells. Toxicol In Vitro. 2016 Sep;35:86-92. [3]. Budu A, et al. Calmidazolium evokes high calcium fluctuations in Plasmodium falciparum. Cell Signal. 2016 Mar;28(3):125-135. |
Molecular Formula |
C31H23CL7N2O
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Molecular Weight |
687.70
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CAS # |
57265-65-3
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Related CAS # |
57265-65-3 (chloride);95013-41-5 (cation);
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SMILES |
ClC1=CC=C(COC(C2=CC=C(Cl)C=C2Cl)CN3C=C[N+](C(C4=CC=C(Cl)C=C4)C5=CC=C(Cl)C=C5)=C3)C(Cl)=C1.[Cl-]
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 100 mg/mL (145.41 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.02 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.02 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.4541 mL | 7.2706 mL | 14.5412 mL | |
5 mM | 0.2908 mL | 1.4541 mL | 2.9082 mL | |
10 mM | 0.1454 mL | 0.7271 mL | 1.4541 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.