| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
Purity: =97.02%
| Targets |
IC50: 10.3 nM (ComD1 receptor)[1]
CSP1 targets the ComD1 receptor, a histidine kinase receptor in the quorum sensing system of Streptococcus pneumoniae. As a competence-stimulating peptide, it activates the ComD1 receptor, triggering a signaling cascade that leads to the expression of competence genes involved in genetic transformation. The compound's potent and selective agonism (IC50 = 10.3 nM) makes it a valuable tool for studying quorum sensing in S. pneumoniae. |
|---|---|
| ln Vitro |
CSP1 demonstrates potent in vitro activity as a ComD1 receptor agonist with an IC50 of 10.3 nM. It regulates genetic transformation of S. pneumoniae by modulating quorum sensing. The compound's activity is concentration-dependent, and its effects on competence development, biofilm formation, and gene expression can be measured in bacterial cultures.
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| ln Vivo |
CSP1 is used in research to study microbial signaling and host-microbe interactions. It plays a crucial role in regulating the genetic transformation of S. pneumoniae. Further in vivo studies are needed to fully characterize its effects on bacterial pathogenesis and potential therapeutic applications as an antimicrobial agent.
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| Enzyme Assay |
In vitro receptor binding assays for CSP1 involve measuring its binding affinity to the ComD1 receptor using surface plasmon resonance or radioligand binding assays. The compound's ability to activate ComD1-mediated signaling can be assessed using reporter gene assays or by measuring downstream gene expression in S. pneumoniae cultures.
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| Cell Assay |
CSP1 activity is assessed in vitro using S. pneumoniae cultures. Bacterial cells are treated with varying concentrations of the peptide, and competence development is measured by assessing genetic transformation efficiency or by measuring the expression of competence genes using qRT-PCR. Biofilm formation can be quantified using crystal violet staining or other biofilm assays.
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| Animal Protocol |
In vivo animal experiments for CSP1 are not extensively documented. If used in animal models of pneumococcal infection, typical protocols would involve administering the peptide to infected animals and evaluating its effects on bacterial colonization, pathogenesis, or host immune responses. Further studies are needed to characterize its in vivo activity.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for CSP1 are limited. As a peptide, its absorption, distribution, metabolism, and excretion properties would be influenced by its peptide nature. The compound is typically used in in vitro research settings. Its stability and bioavailability in biological systems require further investigation.
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| Toxicity/Toxicokinetics |
Toxicological data for CSP1 are limited. As a research peptide, comprehensive toxicological studies have not been reported. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
[1]. Yang Y, et, al. Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumonia. Biochemistry. 2018 Sep 11;57(36):5359-5369.
[2]. Johnsborg O, et, al. A hydrophobic patch in the competence-stimulating Peptide, a pneumococcal competence pheromone, is essential for specificity and biological activity. J Bacteriol. 2006 Mar; 188(5): 1744-9. |
| Additional Infomation |
CSP1 (CAS#: 172889-49-5) is a highly potent and selective agonist of the ComD1 receptor with an IC50 of 10.3 nM. It is a major variant of competence-stimulating peptide (CSP) that regulates genetic transformation of Streptococcus pneumoniae by modulating quorum sensing. The compound is used in research on microbial signaling and host-microbe interactions.
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| Molecular Formula |
C103H168N30O24S
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|---|---|
| Molecular Weight |
2242.69
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| Exact Mass |
2242.26
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| CAS # |
172889-49-5
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| PubChem CID |
155517775
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
-9
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| Hydrogen Bond Donor Count |
34
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| Hydrogen Bond Acceptor Count |
32
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| Rotatable Bond Count |
84
|
| Heavy Atom Count |
158
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| Complexity |
4590
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| Defined Atom Stereocenter Count |
18
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| SMILES |
CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC2=CC=CC=C2)NC(=O)[C@H](CC3=CC=CC=C3)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(=O)O)N
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| InChi Key |
UAYVTMYTRQNBHL-YSTVHMHISA-N
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| InChi Code |
InChI=1S/C103H168N30O24S/c1-8-60(6)83(99(155)131-74(52-59(4)5)92(148)124-70(40-41-80(108)135)90(146)120-67(36-24-47-115-101(109)110)85(141)119-65(33-18-21-44-104)86(142)125-72(100(156)157)35-20-23-46-106)133-97(153)77(55-63-31-16-11-17-32-63)129-96(152)78(56-82(138)139)130-89(145)69(38-26-49-117-103(113)114)122-94(150)75(53-61-27-12-9-13-28-61)128-95(151)76(54-62-29-14-10-15-30-62)127-87(143)66(34-19-22-45-105)123-98(154)79(57-134)132-93(149)73(51-58(2)3)126-88(144)68(37-25-48-116-102(111)112)121-91(147)71(43-50-158-7)118-84(140)64(107)39-42-81(136)137/h9-17,27-32,58-60,64-79,83,134H,8,18-26,33-57,104-107H2,1-7H3,(H2,108,135)(H,118,140)(H,119,141)(H,120,146)(H,121,147)(H,122,150)(H,123,154)(H,124,148)(H,125,142)(H,126,144)(H,127,143)(H,128,151)(H,129,152)(H,130,145)(H,131,155)(H,132,149)(H,133,153)(H,136,137)(H,138,139)(H,156,157)(H4,109,110,115)(H4,111,112,116)(H4,113,114,117)/t60-,64-,65-,66-,67-,68-,69-,70-,71-,72-,73-,74-,75-,76-,77-,78-,79-,83-/m0/s1
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| Chemical Name |
(2S)-6-amino-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S,3S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-carboxybutanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxypropanoyl]amino]hexanoyl]amino]-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-carboxypropanoyl]amino]-3-phenylpropanoyl]amino]-3-methylpentanoyl]amino]-4-methylpentanoyl]amino]-5-oxopentanoyl]amino]-5-carbamimidamidopentanoyl]amino]hexanoyl]amino]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 100 mg/mL (44.59 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (22.29 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.4459 mL | 2.2295 mL | 4.4589 mL | |
| 5 mM | 0.0892 mL | 0.4459 mL | 0.8918 mL | |
| 10 mM | 0.0446 mL | 0.2229 mL | 0.4459 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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