| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| Other Sizes |
| Targets |
Ammonium chloride-15N does not have a specific biological target but serves as a stable isotope tracer for studying nitrogen metabolism and incorporation. When administered to biological systems, the 15N-labeled ammonium is incorporated into amino acids, proteins, nucleic acids, and other nitrogen-containing metabolites. This allows researchers to track nitrogen flux through metabolic pathways using mass spectrometry or NMR spectroscopy.
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|---|---|
| ln Vitro |
In vitro activity of Ammonium chloride-15N is assessed by its utility as a stable isotope tracer in metabolic studies. The compound is used to label cells or tissues with 15N, allowing for the tracking of nitrogen incorporation into proteins, nucleic acids, and metabolites. The efficiency of labeling is assessed by mass spectrometry or NMR analysis. The compound's isotopic purity (>98 atom % 15N) ensures high-quality labeling for research applications.
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| ln Vivo |
In vivo studies for Ammonium chloride-15N are conducted in various animal models and human subjects for metabolic tracer studies. The compound is administered orally or intravenously, and the incorporation of 15N into various metabolites is tracked over time. These studies provide insights into nitrogen metabolism, protein turnover, and amino acid biosynthesis. The compound is widely used in nutritional and metabolic research.
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| Enzyme Assay |
For metabolic labeling studies, Ammonium chloride-15N is dissolved in appropriate media or buffer and added to cell cultures or administered to animals. Cells or tissues are incubated with the labeled compound for defined periods, and samples are collected for analysis. Nitrogen incorporation is assessed by mass spectrometry (GC-MS or LC-MS) or NMR spectroscopy. The isotopic enrichment of amino acids, proteins, or nucleic acids is calculated from mass spectra.
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| Cell Assay |
For cellular studies, cells are cultured in media containing Ammonium chloride-15N as the sole nitrogen source or as a supplement. Cells are incubated for various time points (hours to days) to allow for incorporation of 15N into cellular metabolites. Proteins, amino acids, or nucleic acids are extracted and analyzed by mass spectrometry to determine the extent of 15N incorporation and to study nitrogen metabolism pathways.
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| Animal Protocol |
For in vivo metabolic studies, animals (typically rodents) or human subjects are administered Ammonium chloride-15N via oral gavage, intravenous injection, or dietary incorporation. Blood, urine, and tissue samples are collected at various time points. The 15N enrichment in amino acids, proteins, and other metabolites is measured by mass spectrometry to study nitrogen metabolism, protein turnover, and amino acid kinetics.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Ammonium chloride-15N are similar to those of unlabeled ammonium chloride. Following administration, ammonium is rapidly absorbed and distributed throughout the body. It is metabolized primarily in the liver through the urea cycle and excreted in urine as urea. The 15N label allows for tracking of ammonium metabolism and incorporation into nitrogen-containing compounds. The compound is typically stored at room temperature.
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| Toxicity/Toxicokinetics |
Ammonium chloride-15N is generally considered safe for research use at appropriate concentrations. As a stable isotope-labeled compound, it is not radioactive and poses minimal health risks when handled properly. Standard laboratory safety precautions should be followed when handling this compound. The compound should be stored in a tightly sealed container to prevent contamination.
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| References | |
| Additional Infomation |
Ammonium chloride-15N is a stable isotope-labeled compound with >98 atom % 15N enrichment. It is used as a tracer in metabolic studies, protein labeling, and NMR spectroscopy for tracking nitrogen metabolism. The compound is for research use only and has not been approved for clinical applications. Storage at room temperature in a tightly sealed container is recommended.
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| Molecular Formula |
CLH415N
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|---|---|
| Molecular Weight |
54.48
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| Exact Mass |
54
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| CAS # |
39466-62-1
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| Related CAS # |
Ammonium chloride, AR, 99.5%;12125-02-9
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| PubChem CID |
11051564
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| Appearance |
White to off-white solid powder
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| Melting Point |
340ºC (subl.)(lit.)
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
2
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| Complexity |
0
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[15NH4+].[Cl-]
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| InChi Key |
NLXLAEXVIDQMFP-IEOVAKBOSA-N
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| InChi Code |
InChI=1S/ClH.H3N/h1H;1H3/i;1+1
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| Chemical Name |
azanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 33.33 mg/mL (611.78 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (45.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (45.89 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (45.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 18.3554 mL | 91.7768 mL | 183.5536 mL | |
| 5 mM | 3.6711 mL | 18.3554 mL | 36.7107 mL | |
| 10 mM | 1.8355 mL | 9.1777 mL | 18.3554 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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