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| Other Sizes |
| Targets |
9-Fluorenylmethyl carbazate does not have a specific biological target. Its primary application is as a derivatization reagent for analytical chemistry. Its mechanism of action is based on its ability to react with carbonyl groups (e.g., aldehydes and ketones) on carbohydrates and steroids to form fluorescent derivatives. The carbazate group reacts with the carbonyl to form a hydrazone, which can then be detected by fluorescence or by UV absorbance. This derivatization enhances the sensitivity of detection for these compounds in analytical techniques such as HPLC.
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| ln Vitro |
In vitro, 9-Fluorenylmethyl carbazate is used as a fluorophore reagent for the fluorimetric detection of glycans. It is used as a derivatization reagent for the analysis of various steroids and carbohydrates by high-performance liquid chromatography (HPLC). The compound reacts with carbonyl groups to form fluorescent derivatives that can be detected with high sensitivity. Its use in the analysis of progesterone and other 3-keto steroids demonstrates its utility in analytical chemistry.
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| ln Vivo |
In vivo activity of 9-Fluorenylmethyl carbazate is not studied, as it is a derivatization reagent for analytical chemistry. It is not a bioactive drug and does not have therapeutic applications. Its use is limited to in vitro analytical procedures for the detection and quantification of specific analytes.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays using 9-Fluorenylmethyl carbazate are not typical, as it is a derivatization reagent rather than a binding agent. However, it can be used to derivatize analytes that are then detected in various assays. A standard protocol for derivatization of carbohydrates: a carbohydrate sample is dissolved in a suitable solvent, and 9-fluorenylmethyl carbazate is added in the presence of a catalyst (e.g., acetic acid). The reaction mixture is incubated at a specific temperature for a defined period. The resulting fluorescent hydrazone derivative is then analyzed by HPLC with fluorescence detection. This method is used for the sensitive quantification of carbohydrates in complex samples.
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| Cell Assay |
In vitro cell-based assays for 9-Fluorenylmethyl carbazate are not applicable, as it is not a cell-active compound. It is used in analytical chemistry for the detection of specific analytes in samples that may be derived from cells (e.g., cell culture supernatants or cell lysates). However, the derivatization reaction is performed on the extracted sample, not on living cells.
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| Animal Protocol |
In vivo animal experiments for 9-Fluorenylmethyl carbazate are not performed, as it is a derivatization reagent and not a drug. It is not intended for administration to animals. Its use is limited to in vitro analytical procedures.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 9-Fluorenylmethyl carbazate are not relevant, as it is a reagent for analytical chemistry and not a drug. The compound is typically stored as a solid at refrigerated temperatures (0-10°C). It is soluble in organic solvents and is used in solution for derivatization reactions. Its stability and reactivity are the main considerations for its use.
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| Toxicity/Toxicokinetics |
Toxicity data for 9-Fluorenylmethyl carbazate is limited. As a chemical reagent, it should be handled with standard laboratory precautions. Inhalation, ingestion, and skin contact should be avoided. Appropriate personal protective equipment should be worn. The compound is not intended for human use.
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| References | |
| Additional Infomation |
9-Fluorenylmethyl carbazate is a fluorophore reagent used for fluorescence detection of sugar chains. It is also used as a derivatization reagent for the analysis of progesterone and other 3-keto steroids by HPLC. The compound is also known as Fmoc-hydrazine. It is not a drug and has no clinical use or approval status. It is commercially available from chemical suppliers for research purposes only.
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| Molecular Formula |
C15H14N2O2
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|---|---|
| Molecular Weight |
254.2839
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| Exact Mass |
254.105
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| CAS # |
35661-51-9
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| PubChem CID |
161883
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
485.8±14.0 °C at 760 mmHg
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| Melting Point |
-170ºC (dec.)
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| Flash Point |
247.6±20.1 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.632
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| LogP |
2.88
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
312
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| Defined Atom Stereocenter Count |
0
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| SMILES |
NNC(OCC1C2=C(C3=C1C=CC=C3)C=CC=C2)=O
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| InChi Key |
YGCGPEUVGHDMLO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H14N2O2/c16-17-15(18)19-9-14-12-7-3-1-5-10(12)11-6-2-4-8-13(11)14/h1-8,14H,9,16H2,(H,17,18)
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| Chemical Name |
9H-fluoren-9-ylmethyl N-aminocarbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~196.63 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9327 mL | 19.6634 mL | 39.3267 mL | |
| 5 mM | 0.7865 mL | 3.9327 mL | 7.8653 mL | |
| 10 mM | 0.3933 mL | 1.9663 mL | 3.9327 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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