| Size | Price | Stock | Qty |
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| Targets |
7α-Hydroxycholesterol does not have a single pharmacological target; it is a cholesterol metabolite and oxysterol. Oxysterols are involved in various physiological and pathological processes including cholesterol homeostasis, inflammation, and cell signaling. 7α-Hydroxycholesterol is formed from cholesterol by enzymatic (CYP7A1) and non-enzymatic oxidation. It can be used as a biomarker for lipid peroxidation. Its biological effects are mediated through interactions with nuclear receptors and cell membranes.
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| ln Vitro |
In vitro, 7α-Hydroxycholesterol is used as a research tool and biomarker in studies of cholesterol metabolism and oxidative stress. It is a cholesterol oxide that can be measured in biological samples to assess lipid peroxidation. Its activity is typically evaluated in cell culture models of oxidative stress and inflammation. However, specific potency data are not extensively reported in the available literature, as it is primarily a metabolite and biomarker rather than a drug.
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| ln Vivo |
In vivo, 7α-Hydroxycholesterol is a naturally occurring oxysterol formed from cholesterol oxidation. It is involved in cholesterol metabolism and can be used as a biomarker for lipid peroxidation. Its levels in tissues and body fluids reflect oxidative stress status. As a metabolite, it is not administered as a therapeutic agent but measured as a metabolic marker. Specific in vivo data on its pharmacological effects are not extensively reported.
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| Enzyme Assay |
Cell-free assays for 7α-Hydroxycholesterol involve its detection and quantification in biological samples. HPLC or LC-MS/MS methods are used to measure 7α-Hydroxycholesterol levels in plasma, tissues, or other samples. The compound serves as a reference standard for method development and validation. Its chemical identity is confirmed by comparison with authentic standards. These assays are used for studying cholesterol metabolism and oxidative stress.
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| Cell Assay |
In vitro cellular assays for 7α-Hydroxycholesterol typically involve treating cells with the compound to study its effects on cholesterol metabolism and oxidative stress. Cells are treated with various concentrations of 7α-Hydroxycholesterol, and markers of oxidative stress and inflammation are measured. Cell viability is assessed to determine any cytotoxic effects. However, specific assay protocols are not extensively documented in the available literature. The compound is typically dissolved in ethanol or DMSO and diluted in cell culture medium.
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| Animal Protocol |
In vivo animal studies for 7α-Hydroxycholesterol involve measuring its levels as a biomarker of lipid peroxidation and oxidative stress. Animal models of oxidative stress-related diseases are used. Plasma and tissue samples are collected and analyzed for 7α-Hydroxycholesterol levels using LC-MS/MS. Its levels are correlated with disease progression and treatment effects. These studies help understand the role of cholesterol oxidation in disease.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 7α-Hydroxycholesterol include a molecular weight of 402.66 g/mol and molecular formula C27H46O2. It is soluble in DMSO or ethanol. The compound is typically stored as a powder at -20°C. As a cholesterol metabolite, its pharmacokinetics reflect cholesterol metabolism and oxidation. Detailed ADME parameters are not extensively reported in the available literature.
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| Toxicity/Toxicokinetics |
The toxicity profile of 7α-Hydroxycholesterol is related to its role as an oxysterol. Oxysterols can have cytotoxic and pro-inflammatory effects at elevated concentrations. The compound is used as a research tool and biomarker, not as a therapeutic agent. Standard safety precautions should be followed when handling this compound. It is intended for research use only.
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| References |
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| Additional Infomation |
7α-Hydroxycholesterol (HHC) is a 7α-hydroxy derivative of cholesterol that plays a metabolic role in mice. It is a 7α-hydroxysterol, oxosterosterol, and 3β-hydroxy-Δ5-sterol, functionally related to cholesterol. HHC has been reported in Subergorgia suberosa and Antipathes dichotoma, and relevant data are available.
7α-Hydroxycholesterol is a cholesterol oxide formed from enzymatic and non-enzymatic oxidation of cholesterol. It is an oxysterol that can be used as a biomarker for lipid peroxidation. Its molecular formula is C27H46O2 with a molecular weight of 402.66 g/mol. 7α-Hydroxycholesterol is a research tool for studying cholesterol metabolism and oxidative stress. It is not for therapeutic use. |
| Molecular Formula |
C27H46O2
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|---|---|
| Molecular Weight |
402.65294
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| Exact Mass |
402.35
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| CAS # |
566-26-7
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| PubChem CID |
107722
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| Appearance |
White to off-white solid powder
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| Density |
1.03g/cm3
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| Boiling Point |
515.3ºC at 760mmHg
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| Melting Point |
168-170ºC
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| Flash Point |
214.7ºC
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| Index of Refraction |
1.536
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| LogP |
6.359
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
29
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| Complexity |
624
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| Defined Atom Stereocenter Count |
9
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| SMILES |
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@H]3[C@H](CC[C@]12C)[C@@]4(C)CC[C@@H](CC4=C[C@H]3O)O
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| InChi Key |
OYXZMSRRJOYLLO-RVOWOUOISA-N
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| InChi Code |
InChI=1S/C27H46O2/c1-17(2)7-6-8-18(3)21-9-10-22-25-23(12-14-27(21,22)5)26(4)13-11-20(28)15-19(26)16-24(25)29/h16-18,20-25,28-29H,6-15H2,1-5H3/t18-,20+,21-,22+,23+,24-,25+,26+,27-/m1/s1
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| Chemical Name |
(3S,7S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-3,7-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~124.18 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (24.84 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 100.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 10 mg/mL (24.84 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 100.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4835 mL | 12.4177 mL | 24.8355 mL | |
| 5 mM | 0.4967 mL | 2.4835 mL | 4.9671 mL | |
| 10 mM | 0.2484 mL | 1.2418 mL | 2.4835 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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