| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
7-Amino-4-methylcoumarin is used as a substrate for various proteases, including cathepsin B and leucine aminopeptidase. When conjugated to a peptide, it serves as a fluorogenic probe for enzyme activity. It does not have a specific biological receptor target but is a tool compound.
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|---|---|
| ln Vitro |
In cell-free systems, 7-Amino-4-methylcoumarin is used as a fluorogenic substrate. The non-fluorescent AMC-peptide conjugate is cleaved by the target protease, releasing free AMC, which can be quantified by its fluorescence (excitation 351 nm, emission 430 nm). This provides a direct measure of enzyme activity. In vitro, 7-Amino-4-methylcoumarin exhibits antimicrobial activity against various bacteria and fungi, including Aspergillus, Candida, E. coli, Staphylococcus, Salmonella, and Vibrio. Its primary use, however, is as a fluorescent probe in enzyme activity assays, where it does not exert a direct biological effect on cells.
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| ln Vivo |
In vivo studies are not commonly performed with 7-Amino-4-methylcoumarin as it is primarily a research tool for in vitro applications. Its use as an antimicrobial agent has been studied in vitro, but in vivo efficacy and safety data are not well-documented.
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| Enzyme Assay |
Enzyme assays using 7-Amino-4-methylcoumarin are performed in a cell-free system. A peptide substrate conjugated to AMC is incubated with the target enzyme (e.g., protease) in a suitable buffer. The increase in fluorescence (ex. 351 nm, em. 430 nm) is monitored over time to measure enzymatic activity.
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| Cell Assay |
Cells are treated with an AMC-conjugated peptide substrate. Upon cleavage by intracellular proteases, the released AMC accumulates in the cells and can be detected by fluorescence microscopy or flow cytometry. This allows for the measurement of protease activity within living cells.
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| Animal Protocol |
In vivo studies are generally not applicable. For research purposes, animal models could be used to study the antimicrobial efficacy of the compound, but this is not a standard application. Its primary role is as a biochemical reagent for in vitro and cell-based assays.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for 7-Amino-4-methylcoumarin as it is not a therapeutic agent. It is used as a research tool in biochemical assays, where its stability and fluorescent properties are the primary considerations.
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| Toxicity/Toxicokinetics |
The compound is considered safe for laboratory use. As a fluorescent dye and enzyme substrate, it is not intended for therapeutic use. Its toxicity profile is not well-characterized, but it is handled with standard laboratory safety precautions.
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| References | |
| Additional Infomation |
Coumarin 120 is a member of the 7-aminocoumarin family and functions as a fluorescent dye. Reports have indicated the presence of 7-amino-4-methylcoumarin in tobacco, and relevant data are available for reference.
7-Amino-4-methylcoumarin is a widely used fluorogenic substrate for protease assays. It is also used in histopathological analysis and copper ion detection. It is a key component in many commercial kits for measuring enzyme activity and is a standard tool in biochemistry and cell biology. |
| Molecular Formula |
C10H9NO2
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|---|---|
| Molecular Weight |
175.1840
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| Exact Mass |
175.063
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| CAS # |
26093-31-2
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| PubChem CID |
92249
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| Appearance |
Light yellow to brown solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
378.3±37.0 °C at 760 mmHg
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| Melting Point |
223-226 °C(lit.)
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| Flash Point |
216.9±24.0 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.627
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| LogP |
1.13
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
13
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| Complexity |
260
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GLNDAGDHSLMOKX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
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| Chemical Name |
7-amino-4-methylchromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~570.84 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.27 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 1 mg/mL (5.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: 1 mg/mL (5.71 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7084 mL | 28.5421 mL | 57.0841 mL | |
| 5 mM | 1.1417 mL | 5.7084 mL | 11.4168 mL | |
| 10 mM | 0.5708 mL | 2.8542 mL | 5.7084 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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