| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
7,8-Dihydroneopterin is a heterocyclic pteridine that is generated and secreted by macrophages and monocytes in response to the cytokine interferon-y (IFN-y) [1]. Inhibiting the proliferation of U373MG astrocytes, 7,8-DiHydroneopterin (0.1, 0.5, 1, 2.5, 5 mM; 2 days and 5 days) [1].
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|---|---|
| Cell Assay |
Cell Viability Assay [1]
Cell Types: Astrocyte line U373MG Tested Concentrations: 0.1, 0.5, 1, 2.5, 5 mM Incubation Duration: 2 days and 5 days Experimental Results: After 2 days of treatment, a significant decrease in the number of cells in these cells was observed. 5 mM and 2.5 mM treated cells. After 5 days of incubation, concentrations of 1-5 mM completely eliminated acid phosphatase activity to background levels in complete cell culture medium. Significant cell growth inhibition was also seen for cells incubated with 0.5 mM, whereas 0.1 mM did not alter cell growth. |
| References | |
| Additional Infomation |
7,8-dihydroneopterin is a neopterin where positions C-7 and C-8 have been hydrogenated. It has a role as a metabolite, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite and a mouse metabolite. It is a dihydropterin and a member of neopterins.
7,8-Dihydroneopterin is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). 7,8-Dihydroneopterin has been reported in Homo sapiens and Methanococcus with data available. 7,8-Dihydroneopterin is a metabolite found in or produced by Saccharomyces cerevisiae. |
| Molecular Formula |
C₉H₁₃N₅O₄
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|---|---|
| Molecular Weight |
255.23
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| Exact Mass |
255.097
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| CAS # |
1218-98-0
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| PubChem CID |
135398602
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| Appearance |
Light yellow to yellow solid powder
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| Density |
2.03g/cm3
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| Boiling Point |
614.6ºC at 760mmHg
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| Flash Point |
325.5ºC
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| Vapour Pressure |
1.13E-17mmHg at 25°C
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| Index of Refraction |
1.86
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| LogP |
-3.3
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
470
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C1C(=NC2=C(N1)N=C(NC2=O)N)[C@@H]([C@@H](CO)O)O
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| InChi Key |
YQIFAMYNGGOTFB-XINAWCOVSA-N
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| InChi Code |
InChI=1S/C9H13N5O4/c10-9-13-7-5(8(18)14-9)12-3(1-11-7)6(17)4(16)2-15/h4,6,15-17H,1-2H2,(H4,10,11,13,14,18)/t4-,6+/m1/s1
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| Chemical Name |
2-amino-6-[(1S,2R)-1,2,3-trihydroxypropyl]-7,8-dihydro-3H-pteridin-4-one
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| Synonyms |
7,8Dihydroneopterin; 7,8 Dihydroneopterin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~979.51 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (8.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9180 mL | 19.5902 mL | 39.1803 mL | |
| 5 mM | 0.7836 mL | 3.9180 mL | 7.8361 mL | |
| 10 mM | 0.3918 mL | 1.9590 mL | 3.9180 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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