| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
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| ln Vitro |
6,2'-DiHydroxyflavone is a novel antagonist of GABAA receptors. With a Ki of 37.2±4.5 nM, 6,2'-Dihydroxyflavone prevents [3H]-flunitrazepam from binding to rat cerebral cortex membranes. The current generated by GABA EC50 concentration was lowered to 73.6±1.9% of control when co-administered 5 μM 6,2'-dihydroxyflavone (n=5), whereas that of 1 μM FG was reduced to 65.9±3.0% -7142 (n= 5). The maximal GABA current dropped and the EC50 of the GABA dosage response rose from 47.6 μM to 59.7 μM following the co-administration of 5 μM 6,2'-dihydroxyflavone [1].
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|---|---|
| ln Vivo |
The number of times entering the closed arm [F(4,73)= 0.79, P=0.54] is not as significant as the percentage of 6,2'-dihydroxyflavone-treated mice entering the open arm [F (4,73)=8.01, P<0.0001] or the percentage of time spent in the open arm [F (4,73)=5.19, P<0.002]. At dosages of 8 mg/kg and 16 mg/kg, 6,2'-dihydroxyflavone significantly decreased the percentage of open arm entry and time spent in the open arm, according to Newmane-Keuls post hoc testing. Step latency is similarly increased by 6,2'-dihydroxyflavone treatment [F(4,75)=4.71, P<0.002], suggesting improved cognitive function [1].
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| References |
| Molecular Formula |
C15H10O4
|
|---|---|
| Molecular Weight |
254.2375
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| Exact Mass |
254.058
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| CAS # |
92439-20-8
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| PubChem CID |
688661
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
2.871
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
19
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| Complexity |
390
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YCGXYGWBHFKQHY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H10O4/c16-9-5-6-14-11(7-9)13(18)8-15(19-14)10-3-1-2-4-12(10)17/h1-8,16-17H
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| Chemical Name |
6-hydroxy-2-(2-hydroxyphenyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~393.33 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.83 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL | |
| 5 mM | 0.7867 mL | 3.9333 mL | 7.8666 mL | |
| 10 mM | 0.3933 mL | 1.9666 mL | 3.9333 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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