| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 500mg |
|
||
| 1g |
|
||
| Other Sizes |
| Targets |
6-Aminofluorescein is a fluorescent dye that targets biomolecules for labeling and detection applications. As a fluorescent probe, it is used to label nucleic acids, proteins, and other biomolecules in diagnostic assays. The compound's primary amine group enables covalent conjugation to target molecules. Its fluorescence properties (Ex/Em = 490/515 nm) make it suitable for various imaging and detection applications. The compound is used for labeling fullerene-based liposome nanostructures termed 'buckysomes'.
|
|---|---|
| ln Vitro |
In vitro, 6-Aminofluorescein is used as a fluorescent labeling reagent for biomolecules including nucleic acids and proteins. It has been demonstrated to be a useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed 'buckysomes' (Ex/Em = 490/515 nm). The compound is widely used as a fluorescent probe in biological and chemical assays. Its water solubility makes it suitable for aqueous-based assays. 6-Aminofluorescein is used in diagnostic assays and biomedical research.
|
| ln Vivo |
In vivo, 6-Aminofluorescein is used as a fluorescent marker for imaging and detection applications. The compound has been used for labeling fullerene-based liposome nanostructures for potential in vivo imaging applications. Its fluorescence properties (Ex/Em = 490/515 nm) enable detection in biological systems. 6-Aminofluorescein is used in biomedical research for diagnostic assays and biomolecule labeling. Further in vivo studies are ongoing to fully characterize its utility in imaging applications.
|
| Enzyme Assay |
6-Aminofluorescein fluorescence assays involve measuring its fluorescence properties for labeling and detection applications. The compound's excitation and emission spectra (Ex/Em = 490/515 nm) are characterized using fluorescence spectrophotometry. Labeling efficiency is assessed by measuring fluorescence intensity of conjugated products. For protein labeling, the compound is conjugated to proteins and labeling efficiency is assessed by SDS-PAGE followed by fluorescence imaging. For nucleic acid labeling, similar approaches are used. Assays are performed in appropriate buffer systems with positive controls such as known fluorescent standards.
|
| Cell Assay |
6-Aminofluorescein cell-based assays are conducted in various cell types for labeling and imaging applications. Cells are cultured in appropriate media and treated with 6-Aminofluorescein-conjugated probes. Labeling efficiency is assessed by fluorescence microscopy or flow cytometry. For fullerene-based liposome nanostructure labeling, 'buckysomes' are labeled with 6-Aminofluorescein and cellular uptake is assessed. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls. The compound's water solubility enables aqueous-based cell labeling applications.
|
| Animal Protocol |
6-Aminofluorescein in vivo studies are conducted in animal models for imaging applications. Animals are administered 6-Aminofluorescein-conjugated probes via injection. Tissue distribution and fluorescence signal are assessed by in vivo imaging systems. For fullerene-based liposome nanostructure studies, labeled 'buckysomes' are evaluated for biodistribution and targeting. Animals are monitored for clinical signs. Tissues are collected for ex vivo fluorescence imaging and histopathological analysis at study endpoints. Studies are conducted in accordance with institutional animal care guidelines.
|
| ADME/Pharmacokinetics |
6-Aminofluorescein (MW 347.32 g/mol, C20H13NO5) is a yellow to orange powder. The compound is water-soluble and is stored at +4°C. It is a xanthene-based fluorescent dye derivative of fluorescein. 6-Aminofluorescein is also known as fluoresceinamine isomer II. Pharmacokinetic properties such as half-life, bioavailability, and tissue distribution would be determined in species-specific studies. The compound is typically used as a fluorescent labeling reagent in research applications.
|
| Toxicity/Toxicokinetics |
6-Aminofluorescein is generally well-tolerated as a fluorescent labeling reagent. The compound is a fluorescein derivative with established safety profiles for in vitro and in vivo applications. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
|
| Additional Infomation |
6-Aminofluorescein is a xanthene-based fluorescent dye derivative of fluorescein used as a fluorescent labeling reagent. It is water-soluble and widely used in biological and chemical assays as a fluorescent probe. The compound labels nucleic acids, proteins, and fullerene-based liposome nanostructures ('buckysomes') with Ex/Em = 490/515 nm. 6-Aminofluorescein is used in diagnostic assays and biomedical research. All applications are limited to non-human research use.
|
| Molecular Formula |
C20H13NO5
|
|---|---|
| Molecular Weight |
347.3209
|
| Exact Mass |
347.079
|
| CAS # |
51649-83-3
|
| Related CAS # |
5-Aminofluorescein;3326-34-9
|
| PubChem CID |
103924
|
| Appearance |
Yellow to orange solid powder
|
| Density |
1.6±0.1 g/cm3
|
| Boiling Point |
706.7±60.0 °C at 760 mmHg
|
| Melting Point |
285 °C (dec.)(lit.)
|
| Flash Point |
381.2±32.9 °C
|
| Vapour Pressure |
0.0±2.3 mmHg at 25°C
|
| Index of Refraction |
1.820
|
| LogP |
2.2
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
26
|
| Complexity |
557
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
YOAWSYSKQHLFPM-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
|
| Chemical Name |
5-amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~287.92 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8792 mL | 14.3959 mL | 28.7919 mL | |
| 5 mM | 0.5758 mL | 2.8792 mL | 5.7584 mL | |
| 10 mM | 0.2879 mL | 1.4396 mL | 2.8792 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
