| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg | |||
| Other Sizes |
| Targets |
MCF-7 cells (IC50 = 0.61 μM); SF-268 cells (IC50 = 0.54 μM)
|
|---|---|
| ln Vitro |
6-Methoxydihydrosanguinarine exhibited cytotoxicity against cancer cell lines (e.g., human hepatocellular carcinoma HepG2 and gastric adenocarcinoma BGC-823 cells) in MTT assays, with IC50 values ranging from 1.2 to 4.8 μM. The compound induced apoptosis, as evidenced by increased caspase-3 activity and DNA fragmentation. Mechanistic studies suggested involvement of mitochondrial membrane potential disruption and upregulation of pro-apoptotic Bax protein expression. Additionally, colony formation assays showed significant inhibition of cancer cell clonogenicity. [1]
|
| Cell Assay |
MTT Assay: Cancer cells (HepG2, BGC-823) were seeded in 96-well plates (5,000 cells/well) and treated with 6-Methoxydihydrosanguinarine (0.1–10 μM) for 48 h. Cell viability was measured using MTT reagent, and absorbance at 570 nm was recorded. IC50 values were calculated using dose-response curves.
Apoptosis Detection: Cells treated with 6-Methoxydihydrosanguinarine (2 μM) were stained with Annexin V-FITC/PI and analyzed by flow cytometry to quantify apoptotic cells. Caspase-3 activity was measured using a colorimetric assay kit. Colony Formation Assay: Cells (500 cells/well) were seeded in 6-well plates and treated with 6-Methoxydihydrosanguinarine (1 μM). After 14 days, colonies were fixed, stained with crystal violet, and counted. [1] |
| References | |
| Additional Infomation |
6-Methoxydihydrosandrone is a benzophenanthridine alkaloid isolated from Macleaya cordata. The compound was identified by phytochemical screening and its structure was characterized by nuclear magnetic resonance (NMR) and mass spectrometry (MS). Its cytotoxic activity was compared with other alkaloids from the same plant, highlighting its potential as an anticancer drug. [1]
|
| Molecular Formula |
C21H17NO5
|
|---|---|
| Molecular Weight |
363.36338
|
| Exact Mass |
363.11
|
| CAS # |
72401-54-8
|
| PubChem CID |
14847270
|
| Appearance |
Off-white to pink solid
|
| LogP |
4.1
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
27
|
| Complexity |
575
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
MHPDDMNAUJQRSW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C21H17NO5/c1-22-19-13(4-3-11-7-16-17(8-14(11)19)26-9-25-16)12-5-6-15-20(27-10-24-15)18(12)21(22)23-2/h3-8,21H,9-10H2,1-2H3
|
| Chemical Name |
23-methoxy-24-methyl-5,7,18,20-tetraoxa-24-azahexacyclo[11.11.0.02,10.04,8.014,22.017,21]tetracosa-1(13),2,4(8),9,11,14(22),15,17(21)-octaene
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~34.40 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3.85 mg/mL (10.60 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 38.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7521 mL | 13.7605 mL | 27.5209 mL | |
| 5 mM | 0.5504 mL | 2.7521 mL | 5.5042 mL | |
| 10 mM | 0.2752 mL | 1.3760 mL | 2.7521 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
