| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
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| Targets |
5β-Cholanic acid does not have a specific biological target but is a component of bile acids. Bile acids are synthesized in the liver from cholesterol and are involved in the digestion and absorption of dietary fats. They act as signaling molecules that regulate lipid, glucose, and energy metabolism through the activation of nuclear receptors such as FXR and the G protein-coupled receptor TGR5. By studying 5β-cholanic acid and its derivatives, researchers can gain insights into bile acid biology and metabolism.
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| ln Vitro |
In vitro, 5β-Cholanic acid is used as a reference standard and in studies of bile acid metabolism. The compound's physical and chemical properties, including its solubility and melting point, are characterized for research use. It may be used in cell-based assays to study the effects of bile acids on cell signaling and metabolism. However, specific in vitro activity data are limited, as the compound is primarily used as a research tool.
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| ln Vivo |
In vivo, 5β-Cholanic acid is a component of the bile acid pool and is involved in the enterohepatic circulation of bile acids. It is not typically administered as a therapeutic agent but is used in research on bile acid metabolism and related diseases. The compound's in vivo effects are related to its role in fat digestion and metabolism.
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| Enzyme Assay |
The in vitro assays for 5β-Cholanic acid typically involve chemical characterization and analysis. The compound is analyzed by HPLC, GC, or LC-MS to determine purity and identity. For biological studies, the compound may be tested in cell-based assays for its effects on bile acid receptor activation (e.g., FXR, TGR5) or on cell viability and metabolism. However, specific protocols are not well-documented.
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| Cell Assay |
For in vitro cellular assays, cells (e.g., hepatocytes, intestinal cells) are treated with 5β-Cholanic acid at concentrations ranging from 1 to 100 µM for 24-72 hours. Bile acid receptor activation is assessed by measuring the expression of target genes by qRT-PCR. Cell viability is assessed using MTT or CellTiter-Glo assays. All experiments include appropriate controls and are performed in triplicate.
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| Animal Protocol |
For in vivo studies, 5β-Cholanic acid may be administered to rodents via oral gavage or intraperitoneal injection at doses ranging from 1 to 50 mg/kg. However, specific in vivo protocols for 5β-cholanic acid are not well-documented in publicly available sources. The compound may be used in studies of bile acid metabolism, enterohepatic circulation, or cholestasis. All animal procedures should be conducted in accordance with institutional guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of 5β-Cholanic acid have not been extensively characterized. As a bile acid derivative, it is expected to be absorbed in the intestine, undergo enterohepatic circulation, and be metabolized in the liver. The compound is excreted primarily in bile and feces.
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| Toxicity/Toxicokinetics |
The toxicology of 5β-Cholanic acid has not been extensively characterized. As a bile acid derivative, it is generally considered to have low toxicity at physiological concentrations. High concentrations of bile acids can be cytotoxic and cause cholestasis. The compound is for research use only and is not approved for human therapeutic use.
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| References | |
| Additional Infomation |
5β-cholic acid is a type of cholic acid and belongs to the 5β-cholic acid class of compounds.
5β-Cholanic acid is a bile acid derivative with a molecular formula of C24H40O2 and a molecular weight of 360.58. It is used in research on bile acid metabolism and enterohepatic circulation. The compound is not approved for human use and is intended for research purposes only. It is available as a high-purity research reagent for laboratory use. |
| Molecular Formula |
C24H40O2
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|---|---|
| Molecular Weight |
360.5732
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| Exact Mass |
360.302
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| CAS # |
546-18-9
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| PubChem CID |
92803
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
473.6±13.0 °C at 760 mmHg
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| Flash Point |
208.7±11.2 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
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| Index of Refraction |
1.514
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| LogP |
8.75
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
542
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| Defined Atom Stereocenter Count |
8
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| SMILES |
C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC[C@H]4[C@@]3(CCCC4)C)C
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| InChi Key |
RPKLZQLYODPWTM-LVVAJZGHSA-N
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| InChi Code |
InChI=1S/C24H40O2/c1-16(7-12-22(25)26)19-10-11-20-18-9-8-17-6-4-5-14-23(17,2)21(18)13-15-24(19,20)3/h16-21H,4-15H2,1-3H3,(H,25,26)/t16-,17+,18+,19-,20+,21+,23+,24-/m1/s1
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| Chemical Name |
(4R)-4-[(5S,8R,9S,10S,13R,14S,17R)-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~277.34 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.93 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7734 mL | 13.8669 mL | 27.7339 mL | |
| 5 mM | 0.5547 mL | 2.7734 mL | 5.5468 mL | |
| 10 mM | 0.2773 mL | 1.3867 mL | 2.7734 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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