| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
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| Targets |
5-TAMRA does not have a specific biological target. It is a fluorescent dye used for labeling biomolecules. Its carboxylic acid group enables conjugation to proteins, peptides, and nucleic acids for detection and imaging applications.
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|---|---|
| ln Vitro |
In vitro, 5-TAMRA is characterized by its fluorescent properties, with excitation at ~546 nm and emission at ~579 nm. It is widely used to label peptides and proteins. It is preferred for complicated biological applications where reproducibility is critical.
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| ln Vivo |
In vivo, 5-TAMRA is not used as a therapeutic agent. Its applications are strictly in vitro, for labeling and detection in molecular biology, biochemistry, and imaging applications.
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| Enzyme Assay |
The in vitro labeling protocol for 5-TAMRA involves reacting the dye with the target molecule containing a primary amine group. The labeled product is then purified by chromatography.
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| Cell Assay |
In vitro cell-based assays using 5-TAMRA are primarily for cell staining and imaging. Cells are incubated with the dye or with 5-TAMRA-labeled antibodies or probes, and fluorescence is detected by microscopy or flow cytometry.
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| Animal Protocol |
In vivo animal experiments are not applicable for 5-TAMRA, as it is a fluorescent dye used for labeling and detection in vitro.
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| ADME/Pharmacokinetics |
5-TAMRA has a molecular weight of 430.45 g/mol and is soluble in DMSO. It is typically stored as a powder at -20°C, protected from light.
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| Toxicity/Toxicokinetics |
5-TAMRA is considered to have low toxicity, as it is a fluorescent dye used in diagnostic and laboratory applications. As a research chemical, standard safety precautions should be followed. The product is not intended for therapeutic or human use.
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| Additional Infomation |
5-Carboxytetramethylrhodamine is a tetramethylrhodamine compound with a carboxyl substituent at the 5-position; it is a fluorescent dye.
5-TAMRA is a widely used research reagent with no clinical approval. It is a key tool for labeling peptides and proteins, and for preparing fluorescent bioconjugates. |
| Molecular Formula |
C25H22N2O5
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|---|---|
| Molecular Weight |
430.4526
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| Exact Mass |
430.152
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| CAS # |
91809-66-4
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| PubChem CID |
2762602
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| Appearance |
Green to dark green solid powder
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| LogP |
3.727
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
32
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| Complexity |
875
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YMZMTOFQCVHHFB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
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| Chemical Name |
3-carboxy-4-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 63 mg/mL (~146.36 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (5.81 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3232 mL | 11.6158 mL | 23.2315 mL | |
| 5 mM | 0.4646 mL | 2.3232 mL | 4.6463 mL | |
| 10 mM | 0.2323 mL | 1.1616 mL | 2.3232 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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