| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
5-TAMRA SE does not have a biological target in the traditional sense; it is a fluorescent labeling reagent rather than a drug. Its "target" is primary amine groups (-NH₂) on biomolecules such as peptides, proteins, and nucleic acids. The NHS ester group reacts with primary amines to form stable amide bonds, enabling covalent attachment of the fluorophore to the target molecule. Once conjugated, the TAMRA fluorophore provides a fluorescent label for detection, imaging, and tracking purposes.
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|---|---|
| ln Vitro |
5-TAMRA SE itself is not a biologically active compound with inhibitory or stimulatory effects. Its utility lies in its chemical reactivity and fluorescent properties. It is an amine-reactive probe that produces bright, pH-insensitive orange-red fluorescence with excitation/emission maxima of approximately 545/580 nm (or 546/579 nm). The quantum yield is approximately 0.1. It is nuclease resistant and nuclei permeable, making it suitable for nucleic acid labeling and staining.
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| ln Vivo |
5-TAMRA SE has no inherent in vivo biological activity as it is a labeling reagent. Its utility in vivo depends on conjugation to targeting molecules (e.g., antibodies, peptides) that direct the fluorescent probe to specific tissues or cells. The conjugate's fluorescence can be detected using appropriate imaging systems with excitation/emission at ~545/580 nm. The pH-insensitive fluorescence ensures reliable signal detection across different physiological environments.
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| Enzyme Assay |
There are no enzyme/receptor binding assays for 5-TAMRA SE as it is not a drug targeting a biological receptor. However, the compound's reactivity with primary amines can be assessed in vitro by incubating it with amine-containing molecules (e.g., BSA, peptides) and analyzing the conjugation efficiency using mass spectrometry, SDS-PAGE, or HPLC. Fluorescence of the conjugate is measured to confirm successful labeling and to determine the degree of labeling.
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| Cell Assay |
For cellular labeling experiments, cells are incubated with 5-TAMRA SE-conjugated probes (e.g., antibodies, peptides, or nucleic acids). The compound itself can also be used directly to label surface or intracellular proteins containing primary amines. Following labeling, cells are fixed and imaged using fluorescence microscopy with appropriate filter sets for TAMRA (excitation ~545 nm, emission ~580 nm). Flow cytometry can be used to quantify fluorescence intensity. The pH-insensitive fluorescence ensures reliable detection.
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| Animal Protocol |
In vivo imaging studies using 5-TAMRA SE require conjugation to a targeting moiety (e.g., antibody, peptide) that binds to a specific biomarker. The conjugate is administered to animals (e.g., mice) via intravenous injection. Imaging is performed using an in vivo imaging system (IVIS) with excitation/emission settings appropriate for TAMRA fluorescence. Biodistribution and target engagement are assessed by measuring fluorescence signal in tissues or organs of interest.
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| ADME/Pharmacokinetics |
5-TAMRA SE is a small molecule fluorophore with molecular weight of 527.53 g/mol. It is typically stored as a solid at -20°C under inert gas and protected from light, moisture, and heat. The compound is soluble in DMSO, DMF, and other organic solvents. Once conjugated to proteins, the resulting fluorescent probes are stable and can be used in various biological assays. There is no pharmacokinetic data as it is not administered as a drug.
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| Toxicity/Toxicokinetics |
5-TAMRA SE is considered a research reagent rather than a therapeutic agent, and its toxicity is primarily related to handling and disposal. As a fluorescent dye, it is generally handled with standard laboratory safety precautions to avoid inhalation, ingestion, and skin contact. It may be irritating to eyes and skin. The compound is light, moisture, and heat sensitive and should be stored properly. No systemic toxicity data is available as it is not intended for in vivo administration as a free compound.
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| References | |
| Additional Infomation |
5-TAMRA SE (5-Carboxytetramethylrhodamine succinimidyl ester) is an amine-reactive fluorescent labeling reagent. It is a single-isomer TAMRA derivative with an NHS ester group. It is predominantly used for labeling peptides and proteins. The conjugate produces bright, pH-insensitive orange-red fluorescence with excitation/emission at ~545/580 nm. It is nuclease resistant and nuclei permeable, suitable for DNA/RNA staining.
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| Molecular Formula |
C29H25N3O7
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|---|---|
| Molecular Weight |
527.53
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| Exact Mass |
527.169
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| CAS # |
150810-68-7
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| PubChem CID |
2762600
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| Appearance |
Pale purple to purple solid powder
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| LogP |
3.188
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
39
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| Complexity |
1140
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VWFRSNKRTNUMET-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)19-8-5-16(13-22(19)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3
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| Chemical Name |
2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
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| Synonyms |
5TAMRA SE; 5 TAMRA SE; 5-TAMRA SE
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~5 mg/mL (~9.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (1.90 mM) (saturation unknown) in 10% DMF 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8956 mL | 9.4781 mL | 18.9563 mL | |
| 5 mM | 0.3791 mL | 1.8956 mL | 3.7913 mL | |
| 10 mM | 0.1896 mL | 0.9478 mL | 1.8956 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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