| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
| Targets |
5-Methylcytidine is a modified nucleoside that plays a role in RNA structure and function. It is subject to oxidative processing in vivo to form 5-(hydroxymethyl)cytidine, which has roles in cell viability and development through effects on RNA structure and translation efficiency. It is a key molecule in the study of RNA modifications and epigenetics.
|
|---|---|
| ln Vitro |
In vitro, 5-Methylcytidine is used as a standard for the detection and quantification of modified nucleosides. It is used in studies of RNA modifications, epigenetics, and gene expression. Its levels in biological samples can be measured by LC-MS or other analytical techniques. It is a pyrimidine nucleoside detected in multiple biofluids.
|
| ln Vivo |
In vivo, 5-Methylcytidine is a normal metabolite found in various biofluids. It is present in tRNA and rRNA. Its levels can be affected by various physiological and pathological conditions. It is used as a biomarker in some studies.
|
| Enzyme Assay |
For metabolite quantification, 5-Methylcytidine is measured in biological samples using LC-MS or GC-MS. Samples are processed, and the compound is separated on a suitable column and detected by mass spectrometry. Quantification is performed using external calibration curves or isotope-labeled internal standards.
|
| Cell Assay |
For cellular studies, cells are cultured, and the levels of 5-methylcytidine in the culture medium or cell lysates are measured. Cells may be treated with compounds that affect DNA or RNA methylation, and the resulting changes in 5-methylcytidine levels are measured.
|
| Animal Protocol |
For in vivo studies, biofluid samples (e.g., urine, plasma) are collected from animal models or human subjects. 5-Methylcytidine levels are measured by LC-MS. Its levels are correlated with disease status or treatment response.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for 5-Methylcytidine are not applicable, as it is an endogenous metabolite. Its levels in biofluids reflect its production and clearance rates. No specific PK parameters are reported.
|
| Toxicity/Toxicokinetics |
5-Methylcytidine is a normal metabolite and is not considered toxic at physiological concentrations. No acute toxicity, organ-specific toxicity, or mutagenicity data have been reported. As a research chemical, it should be handled according to standard laboratory safety protocols.
|
| Additional Infomation |
5-Methylcytidine is a methylcytidine.
5-Methylcytidine (CAS 2140-61-6) is a modified nucleoside found in RNA and DNA. It has the molecular formula C₁₀H₁₅N₃O₅ and a molecular weight of 257.24. It is used in epigenetics research and is strictly for research use. |
| Molecular Formula |
C10H15N3O5
|
|---|---|
| Molecular Weight |
257.2432
|
| Exact Mass |
257.101
|
| CAS # |
2140-61-6
|
| PubChem CID |
92918
|
| Appearance |
White to off-white solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
537.5±60.0 °C at 760 mmHg
|
| Melting Point |
212-215 °C (dec.)(lit.)
|
| Flash Point |
278.9±32.9 °C
|
| Vapour Pressure |
0.0±3.2 mmHg at 25°C
|
| Index of Refraction |
1.725
|
| LogP |
-1.35
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
18
|
| Complexity |
422
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
CC1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O
|
| InChi Key |
ZAYHVCMSTBRABG-JXOAFFINSA-N
|
| InChi Code |
InChI=1S/C10H15N3O5/c1-4-2-13(10(17)12-8(4)11)9-7(16)6(15)5(3-14)18-9/h2,5-7,9,14-16H,3H2,1H3,(H2,11,12,17)/t5-,6-,7-,9-/m1/s1
|
| Chemical Name |
4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidin-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~130 mg/mL (~505.36 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3.25 mg/mL (12.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3.25 mg/mL (12.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 3.25 mg/mL (12.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8874 mL | 19.4371 mL | 38.8742 mL | |
| 5 mM | 0.7775 mL | 3.8874 mL | 7.7748 mL | |
| 10 mM | 0.3887 mL | 1.9437 mL | 3.8874 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
