| Size | Price | |
|---|---|---|
| 100mg | ||
| Other Sizes |
| Targets |
5-Methyl-7-methoxyisoflavone targets pathways involved in muscle growth and cancer cell survival. It increases muscle mass and endurance, suggesting anabolic effects. It inhibits the clonogenic survival of HCT116 colon cancer cells at concentrations of 10, 20, and 40 µM. As a non-steroidal isoflavone, it may interact with estrogen receptors or other signaling pathways involved in muscle growth and cancer cell proliferation.
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|---|---|
| ln Vitro |
In vitro, 5-Methyl-7-methoxyisoflavone inhibits the clonogenic survival of HCT116 cells when used at concentrations of 10, 20, and 40 µM. It increases muscle mass and endurance in cell-based or tissue-based models. Quantitative IC50 values for its effects on cancer cell survival are not detailed in the publicly available sources.
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| ln Vivo |
In vivo, 5-Methyl-7-methoxyisoflavone increases muscle mass and endurance. It is a non-steroidal anabolic isoflavone. Detailed dose-response and pharmacokinetic data in animal models are limited in publicly available literature. Further research is needed to fully characterize its in vivo activity and therapeutic potential.
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| Enzyme Assay |
For in vitro cell-free assays, the anabolic activity of 5-Methyl-7-methoxyisoflavone can be studied by measuring its effects on muscle cell proliferation or protein synthesis in cell-free systems. Its effects on cancer cell survival can be assessed using clonogenic survival assays. Receptor binding studies can be performed to identify its molecular targets.
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| Cell Assay |
For in vitro cellular assays, the effects of 5-Methyl-7-methoxyisoflavone on muscle cells are assessed by measuring cell proliferation, differentiation, and protein synthesis. Its anticancer activity is assessed in HCT116 colon cancer cells by clonogenic survival assays at concentrations of 10, 20, and 40 µM. Cell viability is measured using MTT or similar assays.
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| Animal Protocol |
For in vivo studies, 5-Methyl-7-methoxyisoflavone could be administered orally to animal models to assess its effects on muscle mass and endurance. Endpoints include muscle weight, grip strength, and exercise performance. In cancer models, tumor growth inhibition could be assessed.
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| ADME/Pharmacokinetics |
5-Methyl-7-methoxyisoflavone (CAS 82517-12-2) has a molecular formula of C17H14O3 and a molecular weight of 266.29 g/mol. It is also known as 7-Methoxy-5-methyl-3-phenyl-4H-1-benzopyran-4-one. Appearance: typically a powder. Solubility: soluble in ethanol, DMSO, and other organic solvents. Storage: typical for isoflavones (desiccated, protected from light, -20°C). Purity: typically >98% for research use.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is publicly available. As a synthetic isoflavone, its safety profile would need to be established through standard toxicological studies. In vitro cytotoxicity assays in various cell lines are typically performed alongside efficacy studies to confirm that observed effects are not due to a general reduction in cell viability.
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| References | |
| Additional Infomation |
5-Methyl-7-methoxyisoflavone is a research-grade compound and is not approved for therapeutic use. It serves primarily as a pharmacological tool for studying muscle growth, anabolic effects, and cancer cell biology. Its mechanism of action involves increasing muscle mass and endurance. It inhibits the clonogenic survival of HCT116 cells. No clinical trials have been reported.
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| Molecular Formula |
C17H14O3
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|---|---|
| Molecular Weight |
266.2913
|
| Exact Mass |
266.094
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| CAS # |
82517-12-2
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| PubChem CID |
2734290
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
449.3±45.0 °C at 760 mmHg
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| Melting Point |
116-120 °C
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| Flash Point |
217.5±15.1 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.606
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| LogP |
3.95
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
20
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| Complexity |
395
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
WGOUYULOZZRTFS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C17H14O3/c1-11-8-13(19-2)9-15-16(11)17(18)14(10-20-15)12-6-4-3-5-7-12/h3-10H,1-2H3
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| Chemical Name |
7-methoxy-5-methyl-3-phenylchromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~469.41 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (23.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7553 mL | 18.7765 mL | 37.5530 mL | |
| 5 mM | 0.7511 mL | 3.7553 mL | 7.5106 mL | |
| 10 mM | 0.3755 mL | 1.8777 mL | 3.7553 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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