| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
5-Ethynyl-2'-deoxyuridine targets newly synthesized DNA. As a thymidine analog, it is incorporated into DNA during the S-phase of the cell cycle in place of thymidine. The ethynyl group enables subsequent detection via click chemistry. EdU does not bind to specific proteins but rather serves as a metabolic label for DNA synthesis. It is widely used to study cell proliferation, DNA replication, and cell cycle dynamics in various biological systems.
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| ln Vitro |
5-Ethynyl-2'-deoxyuridine (EdU) staining is a quick, accurate, and repeatable technique for examining the proliferation of cells in the central nervous system [1].
In vitro, 5-Ethynyl-2'-deoxyuridine is used to label proliferating cells in culture. Cells are incubated with EdU for a defined labeling period (typically 30 minutes to several hours), during which EdU is incorporated into newly synthesized DNA. After fixation and permeabilization, the incorporated EdU is detected using a copper-catalyzed azide-alkyne cycloaddition (CuAAc) reaction with a fluorescent azide dye. This allows for the identification and quantification of cells that were in S-phase during the labeling period. EdU labeling is compatible with antibody staining for simultaneous detection of other cellular markers. |
| ln Vivo |
Two-month-old female mice's dentate gyrus showed a little increase in the amount of 5-Ethynyl-2'-deoxyuridine (EdU; one intraperitoneal injection of EdU at 10, 20, 50, 100, or 200 mg/kg)-positive cells [1].
In vivo, 5-Ethynyl-2'-deoxyuridine can be administered to animals to label proliferating cells in tissues. EdU is typically injected intraperitoneally or intravenously, and after a defined labeling period, tissues are collected and processed for detection. The incorporated EdU is detected via click chemistry on tissue sections, allowing for the identification of proliferating cells in specific tissues or organs. This technique is widely used in developmental biology, cancer research, and neurogenesis studies. |
| Enzyme Assay |
Non-cellular enzyme/receptor binding assay protocols are not applicable to EdU, as it is not an enzyme inhibitor or receptor ligand. However, chemical characterization of EdU involves HPLC, NMR, and mass spectrometry to confirm purity and structure. The compound is typically provided as a solid and dissolved in DMSO or PBS for use. Its reactivity in click chemistry can be assessed by reaction with azide-containing fluorophores, with reaction efficiency monitored by HPLC or fluorescence spectroscopy.
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| Cell Assay |
Cellular assay protocols for 5-Ethynyl-2'-deoxyuridine involve labeling cells with EdU. Cells are incubated with EdU at concentrations typically ranging from 1-50 μM for 30 minutes to 24 hours. After labeling, cells are fixed, permeabilized, and incubated with a click chemistry reaction cocktail containing a fluorescent azide, copper catalyst, and reducing agent. The fluorescent signal is detected by flow cytometry, fluorescence microscopy, or plate reader. DNA counterstains (e.g., DAPI or Hoechst) are used to identify total cell nuclei.
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| Animal Protocol |
Animal/Disease Models: Two month old female mice[1]
Doses: 10, 20, 50, 100 or 200 mg/kg Route of Administration: Single injection; intraperitoneally; 4 hrs (hours) after EdU injection, brains were processed for EdU staining. Experimental Results: EdU positive cell Numbers slightly increased in a dose-dependent manner both in control and running mice. In vivo animal experiment protocols for 5-Ethynyl-2'-deoxyuridine involve administering EdU to animals. EdU is typically injected intraperitoneally or intravenously at doses of 10-100 mg/kg. After a labeling period (hours to days), animals are euthanized and tissues are collected, fixed, and sectioned. The incorporated EdU is detected via click chemistry on tissue sections. This allows for the mapping of proliferating cells in tissues. EdU labeling can be combined with immunohistochemistry for simultaneous detection of cell-type specific markers. |
| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-Ethynyl-2'-deoxyuridine have been characterized. As a small nucleoside analog (MW 252.22 g/mol), EdU is cell-permeable and rapidly incorporated into DNA. It is soluble in DMSO (≥60 mg/mL). In vivo, EdU is distributed to tissues and incorporated into proliferating cells. The compound is metabolized and cleared from the body over time. The optimal labeling time depends on the specific application and tissue type.
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| Toxicity/Toxicokinetics |
Toxicity of 5-Ethynyl-2'-deoxyuridine is generally low at the concentrations used for labeling studies. EdU is considered less toxic than BrdU and does not require DNA denaturation for detection. However, like other nucleoside analogs, high concentrations or prolonged exposure may affect cell proliferation or cause DNA damage. Standard safety precautions should be followed when handling EdU. The compound is for research use only and not for human therapeutic applications.
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| References |
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| Additional Infomation |
5-Ethynyl-2'-deoxyuridine (CAS: 61135-33-9) has a molecular formula of C11H12N2O5 and a molecular weight of 252.22 g/mol. It is also known as EdU. It is a thymidine analog used to monitor de novo DNA synthesis through click chemistry. EdU is a replacement for BrdU to measure DNA synthesis during S-phase of the cell cycle. Purity is typically ≥95%. Solubility: DMSO ≥60 mg/mL. This product is for research use only.
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| Molecular Formula |
C11H12N2O5
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| Molecular Weight |
252.22338
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| Exact Mass |
252.075
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| CAS # |
61135-33-9
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| PubChem CID |
472172
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| Appearance |
White to yellow solid powder
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| Density |
1.55g/cm3
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| Melting Point |
199 °C
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| Index of Refraction |
1.644
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| LogP |
-1.3
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
463
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C#CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
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| InChi Key |
CDEURGJCGCHYFH-DJLDLDEBSA-N
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| InChi Code |
InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1
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| Chemical Name |
5-ethynyl-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~198.24 mM)
H2O : ~25 mg/mL (~99.12 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 4 mg/mL (15.86 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9648 mL | 19.8240 mL | 39.6479 mL | |
| 5 mM | 0.7930 mL | 3.9648 mL | 7.9296 mL | |
| 10 mM | 0.3965 mL | 1.9824 mL | 3.9648 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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