| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| 2g |
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| 5g |
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| Other Sizes |
5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye with a wide-range of applications in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA, as a label for antibodies and other probes.
| Cell Assay |
- Antibody labeling assay: Antibodies were dialyzed against carbonate buffer (pH 9.0) to adjust the pH. 5(6)-FITC was dissolved in anhydrous solvent and added dropwise to the antibody solution at a molar ratio of antibody:FITC = 1:20–1:50. The mixture was incubated at 4°C for 4–6 hours in the dark, then passed through a gel filtration column to separate labeled antibodies from free 5(6)-FITC [1]
- Fab' fragment labeling assay: Fab' fragments with a single free thiol group were prepared. 5(6)-FITC was activated to form a thiol-reactive derivative, then incubated with Fab' fragments in phosphate buffer (pH 7.4) at 25°C for 2 hours in the dark. Unconjugated 5(6)-FITC was removed by dialysis to obtain FITC-labeled Fab' while preserving the thiol group [2] - DNA fluorescence detection assay: 5(6)-FITC was conjugated to DNA primers or nucleotides. The labeled DNA samples were subjected to automated sequence analysis, and fluorescence signals emitted by 5(6)-FITC (excitation ~495 nm, emission ~520 nm) were detected by a fluorescence detector to determine DNA sequences [3] |
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
Cyanide can be rapidly absorbed and distributed throughout the body via oral ingestion, inhalation, and skin contact. Cyanide is primarily metabolized to thiocyanate by thiocyanate oxidase or 3-mercaptopyruvate-thiotransferase. Cyanide metabolites are excreted in the urine. (L96) |
| Toxicity/Toxicokinetics |
Toxicity Summary
Cyanide is an inhibitor of cytochrome c oxidase in the fourth electron transport complex (located on the mitochondrial membrane of eukaryotic cells). It forms a complex with the ferric iron atom in this enzyme. The binding of cyanide to this cytochrome prevents electrons from being transferred from cytochrome c oxidase to oxygen. As a result, the electron transport chain is disrupted, and the cell can no longer perform aerobic respiration to produce ATP for energy. Tissues that rely primarily on aerobic respiration, such as the central nervous system and the heart, are particularly susceptible to this effect. Cyanide is also known to exert some toxic effects by binding to catalase, glutathione peroxidase, methemoglobin, hydroxycobalamin, phosphatase, tyrosinase, ascorbic acid oxidase, xanthine oxidase, succinate dehydrogenase, and copper/zinc superoxide dismutase. Cyanide binds to the iron ions in methemoglobin to form inactive cyanomethemoglobin. (L97) |
| References |
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| Additional Infomation |
5-Fluorescence 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. It is a fluorescent probe that can be conjugated to tissues and proteins; it can be used as a marker in fluorescent antibody staining and protein and amino acid binding techniques. Fluorescence 5-isothiocyanate is a cyanide-containing compound derived from fluorescein. It is used in flow cytometry. (L552) A fluorescent probe that can be conjugated to tissues and proteins. It is used as a marker in fluorescent antibody staining procedures and protein and amino acid binding techniques.
- 5(6)-FITC (5(6)-fluorescein isothiocyanate) is a fluorescent labeling reagent with a fluorescein core structure, existing in the form of a mixture of 5-isomers and 6-isomers [1][2][3] - Its main function is to couple biomolecules (antibodies, Fab' fragments, DNA, etc.) by reacting the isothiocyanate group (-NCS) with the amino or thiol group of the target molecule [1][2][3] - It has strong fluorescence at an excitation wavelength of ~495 nm and an emission wavelength of ~520 nm, and can sensitively detect labeled biomolecules [3] |
| Molecular Formula |
C21H11NO5S
|
|---|---|
| Molecular Weight |
389.3807
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| Exact Mass |
389.035
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| CAS # |
27072-45-3
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| Related CAS # |
63469-13-6 (hydrochloride)
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| PubChem CID |
18730
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| Appearance |
Yellow to orange solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
708.6±60.0 °C at 760 mmHg
|
| Melting Point |
>360ºC(lit.)
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| Flash Point |
382.4±32.9 °C
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| Vapour Pressure |
0.0±2.3 mmHg at 25°C
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| Index of Refraction |
1.754
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| LogP |
4
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
28
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| Complexity |
668
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
|
| Chemical Name |
3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 156 mg/mL (~400.64 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.34 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5682 mL | 12.8409 mL | 25.6819 mL | |
| 5 mM | 0.5136 mL | 2.5682 mL | 5.1364 mL | |
| 10 mM | 0.2568 mL | 1.2841 mL | 2.5682 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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