| Size | Price | |
|---|---|---|
| 500mg | ||
| Other Sizes |
| ln Vitro |
5,6-Dihydroxyindole (1, 10, 20, 50, 100 μM; for 4 hours) decreased OD492 readings in a dose-dependent manner. For four hours, 1.0 mM 5,6-dihydroxyindole was used as a pretreatment on Sf9 cells. 97% of the population died. The LC50 values in the culture medium were 131.8 μM and 20.3 μM, respectively, in the buffer [1]. The baculovirus stock produced nearly no recombinant protein after three hours of preincubation with 1.25 mM 5,6-dihydroxyindole. Eggs of the wasp Microplitis demolitor and the lambda phage have LC50 values of 111.0 μM and 5.6 μM, respectively [1]. Additionally effective against viruses and parasitic wasps are 5,6-dihydroxyindole and its spontaneous oxidation products [1].
|
|---|---|
| ADME/Pharmacokinetics |
Metabolism / Metabolites
Dihyroxy-1H-indole has known human metabolites that include Dihyroxy-1H-indole glucuronide I. |
| References |
|
| Additional Infomation |
5,6-dihydroxyindole is a dihydroxyindole. It has a role as a mouse metabolite.
5,6-Dihydroxyindole has been reported in Homo sapiens and Trypanosoma brucei with data available. |
| Molecular Formula |
C₈H₇NO₂
|
|---|---|
| Molecular Weight |
149.15
|
| Exact Mass |
149.047
|
| CAS # |
3131-52-0
|
| Related CAS # |
25656-67-1
|
| PubChem CID |
114683
|
| Appearance |
Off-white to light brown solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
411.2±25.0 °C at 760 mmHg
|
| Flash Point |
202.5±23.2 °C
|
| Vapour Pressure |
0.0±1.0 mmHg at 25°C
|
| Index of Refraction |
1.802
|
| LogP |
0.37
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
11
|
| Complexity |
151
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
SGNZYJXNUURYCH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H7NO2/c10-7-3-5-1-2-9-6(5)4-8(7)11/h1-4,9-11H
|
| Chemical Name |
1H-indole-5,6-diol
|
| Synonyms |
5,6Dihydroxyindole; 5,6 Dihydroxyindole
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~335.23 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (8.38 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (8.38 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (8.38 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL | |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL | |
| 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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