| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
|
||
| Other Sizes |
| Targets |
5(6)-Carboxyfluorescein does not have a specific therapeutic target but serves as a fluorescent probe for labeling biomolecules and measuring intracellular pH. Its carboxyl group reacts with amines to form stable amide bonds, allowing it to be conjugated to proteins, peptides, and other biomolecules. Its fluorescence is pH-sensitive, making it useful for measuring changes in intracellular pH. It does not have pharmacological activity but is a valuable tool for fluorescence-based applications in research.
|
|---|---|
| ln Vitro |
5(6)-Carboxyfluorescein has two key characteristics: it has two maximum absorbance wavelengths (465 and 490 nm), and its fluorescence emission (maximum 515 nm) varies with pH in the physiological pH range 6-7.4. increases due to modifications [2].
In vitro, 5(6)-Carboxyfluorescein is used as a fluorescent probe for labeling biomolecules. It is used for measuring changes in intracellular pH. The compound's fluorescence is excited at approximately 488 nm and emits at approximately 520 nm. It is used in flow cytometry, fluorescence microscopy, and other fluorescence-based applications. Its amine-reactive property allows it to be conjugated to antibodies, proteins, and other molecules for detection and imaging. |
| ln Vivo |
5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be applied to the mapping of tumor tissue's pH in vivo [2].
In vivo, 5(6)-Carboxyfluorescein is not used as a therapeutic agent. Its application is limited to in vitro research applications. The compound is not administered to animals or humans for therapeutic purposes. Its use is strictly confined to laboratory settings. |
| Enzyme Assay |
In vitro non-cell assays for 5(6)-Carboxyfluorescein are not applicable, as it is a fluorescent dye. Its properties are characterized by its spectral characteristics (excitation and emission maxima) and its pH sensitivity. Its purity is assessed by HPLC or other analytical methods.
|
| Cell Assay |
In vitro cell-based assays for 5(6)-Carboxyfluorescein involve using the dye to label cells or biomolecules for fluorescence-based detection. Cells can be loaded with the dye or its derivatives to measure intracellular pH. The dye can also be conjugated to antibodies or other probes for immunostaining or flow cytometry. Its fluorescence is measured using a fluorescence plate reader, flow cytometer, or fluorescence microscope.
|
| Animal Protocol |
Animal/Disease Models: CDF mice, lymphoid leukemia P388[2]
Doses: 5 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: Can be used for measurement and imaging of tumor tissue. In vivo animal studies are not typically performed with 5(6)-Carboxyfluorescein, as it is a fluorescent dye used in cell-based assays. Its use is limited to in vitro and ex vivo applications. |
| ADME/Pharmacokinetics |
5(6)-Carboxyfluorescein has a molecular weight of 376.32 g/mol (for the free acid) and a molecular formula of C₂₁H₁₂O₇ (for each isomer). It is also known as 5(6)-FAM. The compound appears as a powder and should be stored under appropriate conditions, typically at -20°C, protected from light and moisture. It is soluble in DMSO and other organic solvents. Its stability in solution is good under proper storage conditions.
|
| Toxicity/Toxicokinetics |
5(6)-Carboxyfluorescein is considered a safe laboratory reagent when handled appropriately. It is not intended for human or veterinary use. Standard laboratory safety precautions should be followed when handling the compound, including the use of appropriate personal protective equipment.
|
| References |
|
| Additional Infomation |
5(6)-Carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. It is a fluorescent dye commonly used as a tracer. It is both a tracer and a fluorescent dye. It contains 5-carboxyfluorescein and 6-carboxyfluorescein.
5(6)-Carboxyfluorescein is a mixed-isomer green fluorescent dye used for labeling biomolecules and measuring intracellular pH. It is also known as 5(6)-FAM. The compound is amine-reactive and pH-sensitive. Not a therapeutic agent; intended for research purposes only. |
| Molecular Formula |
C42H24O14
|
|---|---|
| Molecular Weight |
752.6316
|
| Exact Mass |
752.116
|
| CAS # |
72088-94-9
|
| PubChem CID |
44119975
|
| Appearance |
Yellow to orange solid powder
|
| Melting Point |
275 °C
|
| LogP |
6.728
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
14
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
56
|
| Complexity |
1270
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
BPVHBBXCESDRKW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)
|
| Chemical Name |
3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid;3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~83.04 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (2.76 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3287 mL | 6.6434 mL | 13.2867 mL | |
| 5 mM | 0.2657 mL | 1.3287 mL | 2.6573 mL | |
| 10 mM | 0.1329 mL | 0.6643 mL | 1.3287 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
