| Size | Price | Stock | Qty |
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| 1g |
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| Other Sizes |
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5(6)-Carboxyfluorescein is a novel and potent carboxylic acid used to react with primary amines via carbodiimide activation of the carboxylic acid; cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.
| ln Vitro |
5(6)-Carboxyfluorescein has two key characteristics: it has two maximum absorbance wavelengths (465 and 490 nm), and its fluorescence emission (maximum 515 nm) varies with pH in the physiological pH range 6-7.4. increases due to modifications [2].
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|---|---|
| ln Vivo |
5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be applied to the mapping of tumor tissue's pH in vivo [2].
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| Animal Protocol |
Animal/Disease Models: CDF mice, lymphoid leukemia P388[2]
Doses: 5 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: Can be used for measurement and imaging of tumor tissue. |
| References |
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| Additional Infomation |
5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.
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| Molecular Formula |
C42H24O14
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|---|---|
| Molecular Weight |
752.6316
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| Exact Mass |
752.116
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| CAS # |
72088-94-9
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| PubChem CID |
44119975
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| Appearance |
Yellow to orange solid powder
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| Melting Point |
275 °C
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| LogP |
6.728
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
56
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| Complexity |
1270
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BPVHBBXCESDRKW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)
|
| Chemical Name |
3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid;3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~83.04 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (2.76 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3287 mL | 6.6434 mL | 13.2867 mL | |
| 5 mM | 0.2657 mL | 1.3287 mL | 2.6573 mL | |
| 10 mM | 0.1329 mL | 0.6643 mL | 1.3287 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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