| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
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| Other Sizes |
| ln Vitro |
4-Methylherniarin exhibited in vitro antibacterial activity against both Gram-positive and Gram-negative bacterial strains [1]
It showed significant inhibitory effects on Staphylococcus aureus, with a minimum inhibitory concentration (MIC) of 62.5 μg/mL [1] Against Escherichia coli, the compound had an MIC value of 125 μg/mL, demonstrating moderate antibacterial potency [1] In the disc diffusion assay, 4-Methylherniarin produced clear inhibition zones around bacterial colonies, with zone diameters of ~12 mm (S. aureus) and ~10 mm (E. coli) at a concentration of 250 μg/disc [1] |
|---|---|
| Cell Assay |
Bacterial strains (Staphylococcus aureus, Escherichia coli) were cultured in Mueller-Hinton broth (MHB) at 37°C overnight to reach logarithmic growth phase [1]
4-Methylherniarin was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (10 mg/mL), then serially diluted with MHB to concentrations ranging from 15.625 to 500 μg/mL [1] #### Minimum Inhibitory Concentration (MIC) Assay (Microdilution Method) - 96-well microtiter plates were loaded with 100 μL of diluted 4-Methylherniarin solutions and 100 μL of bacterial suspension (1×10⁶ CFU/mL) [1] - Plates were incubated at 37°C for 24 hours; the MIC was defined as the lowest concentration of the compound that completely inhibited bacterial growth (no visible turbidity) [1] - Negative controls contained MHB + bacterial suspension + DMSO (final DMSO concentration < 1%), and positive controls contained MHB + bacterial suspension + standard antibiotic [1] #### Disc Diffusion Assay - Sterile filter discs (6 mm diameter) were impregnated with 20 μL of 4-Methylherniarin stock solution (250 μg/disc) and air-dried [1] - Mueller-Hinton agar (MHA) plates were inoculated with bacterial suspension (1×10⁶ CFU/mL) to form a uniform lawn [1] - Impregnated discs were placed on the inoculated agar plates, incubated at 37°C for 24 hours, and the diameter of inhibition zones was measured in millimeters [1] |
| References | |
| Additional Infomation |
7-Methoxy-4-methyl-1-benzopyran-2-one is a coumarin compound. 4-Methylhenialine is a natural coumarin derivative with a benzene-fused α-pyranone structure [1]. It is classified as a coumarin analog and its antibacterial activity is attributed to its coumarin core structure, which may interact with bacterial cell membrane components or essential enzymes [1]. This study aims to evaluate its potential as a lead compound for developing novel antibacterial agents, particularly against common pathogens [1].
|
| Molecular Formula |
C11H10O3
|
|---|---|
| Molecular Weight |
190.1953
|
| Exact Mass |
190.062
|
| CAS # |
2555-28-4
|
| PubChem CID |
390807
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
340.1±37.0 °C at 760 mmHg
|
| Melting Point |
158-160 °C(lit.)
|
| Flash Point |
140.8±21.1 °C
|
| Vapour Pressure |
0.0±0.7 mmHg at 25°C
|
| Index of Refraction |
1.556
|
| LogP |
2.37
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
14
|
| Complexity |
270
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
UDFPKNSWSYBIHO-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C11H10O3/c1-7-5-11(12)14-10-6-8(13-2)3-4-9(7)10/h3-6H,1-2H3
|
| Chemical Name |
7-methoxy-4-methylchromen-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~131.44 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (10.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2576 mL | 26.2881 mL | 52.5762 mL | |
| 5 mM | 1.0515 mL | 5.2576 mL | 10.5152 mL | |
| 10 mM | 0.5258 mL | 2.6288 mL | 5.2576 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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