| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
4'-Methoxyflavonol targets oxidative stress pathways, acting as a radical scavenger. It reduces androgen receptor levels and increases superoxide dismutase (SOD) activity. Its effects on plasma glucose, cholesterol, and triglycerides suggest it modulates metabolic pathways. Its primary mechanism is believed to be its antioxidant activity, which may have downstream effects on various cellular processes.
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|---|---|
| ln Vitro |
In vitro, 4'-Methoxyflavonol exhibits potent antioxidant activity, as demonstrated by its ability to scavenge DPPH radicals (IC₅₀ = 25.54 µg/ml). It reduces androgen receptor levels and increases SOD activity. It also shows anti-stress and antioxidant activities. Its activity is primarily attributed to its radical-scavenging capacity conferred by the 3-hydroxy group.
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| ln Vivo |
In vivo, 4'-Methoxyflavonol has been shown to reduce plasma glucose, cholesterol, and triglycerides. This suggests that its antioxidant and metabolic effects translate to systemic benefits in animal models. It also has anti-stress activity. These findings indicate its potential for further study in metabolic and stress-related disorders.
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| Enzyme Assay |
In vitro non-cell enzyme/receptor binding assays for 4'-Methoxyflavonol typically involve measuring its antioxidant activity. This is done using cell-free assays such as the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, where the compound's ability to neutralize the stable DPPH radical is measured spectrophotometrically. The IC₅₀ is determined from a dose-response curve.
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| Cell Assay |
In vitro cell-based assays for 4'-Methoxyflavonol use various cell lines to study its effects on oxidative stress. Cells are treated with the compound and exposed to an oxidative challenge, and markers of oxidative stress, such as reactive oxygen species (ROS) levels and SOD activity, are measured. Its effects on androgen receptor expression can also be assessed in relevant cell lines via Western blot or qPCR.
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| Animal Protocol |
In vivo animal studies for 4'-Methoxyflavonol would likely involve administering the compound to animal models of metabolic syndrome or stress. Parameters such as plasma glucose, cholesterol, and triglycerides would be measured to assess its metabolic effects. Behavioral tests might be used to evaluate its anti-stress activity.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
Known metabolites of 3-hydroxy-4p-methoxyflavonoids include (2S,3S,4S,5R)-3,4,5-trihydroxy-6-[2-(4-methoxyphenyl)-4-oxochromen-3-yl]oxooxacyclohexane-2-carboxylic acid. 4'-Methoxyflavonol has a molecular weight of 268.26 g/mol and a molecular formula of C₁₆H₁₂O₄. It is a synthetic flavonol. It is soluble in organic solvents such as DMSO. Detailed pharmacokinetic data, such as absorption, distribution, metabolism, and excretion (ADME), are not widely published. |
| Toxicity/Toxicokinetics |
The toxicity profile of 4'-Methoxyflavonol is not extensively detailed in the provided search results. As a flavonol, it is generally considered to have a good safety profile, but comprehensive toxicological studies are needed for therapeutic development. It is not intended for human therapeutic use without further development.
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| Additional Infomation |
3-hydroxy-4'-methoxyflavonoids have been reportedly found in Millettia zechiana, and relevant data are available.
4'-Methoxyflavonol is a synthetic flavonol with potent antioxidant activity (DPPH IC₅₀ = 25.54 µg/ml). It reduces androgen receptor and increases SOD, and reduces plasma glucose, cholesterol, and triglycerides. It has anti-stress and antioxidant activities. It is not approved for clinical use and is intended for research purposes only. |
| Molecular Formula |
C16H12O4
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|---|---|
| Molecular Weight |
268.2641
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| Exact Mass |
268.074
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| CAS # |
6889-78-7
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| PubChem CID |
97141
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.353g/cm3
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| Boiling Point |
426.7ºC at 760 mmHg
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| Melting Point |
233-234ºC
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| Flash Point |
160.7ºC
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| Index of Refraction |
1.651
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| LogP |
3.174
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
20
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| Complexity |
407
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C2=C([H])C([H])=C([H])C([H])=C2C(C(=C1C1C([H])=C([H])C(=C([H])C=1[H])OC([H])([H])[H])O[H])=O
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| InChi Key |
IIBBFGMVMNZMGA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H12O4/c1-19-11-8-6-10(7-9-11)16-15(18)14(17)12-4-2-3-5-13(12)20-16/h2-9,18H,1H3
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| Chemical Name |
3-hydroxy-2-(4-methoxyphenyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~6.4 mg/mL (~23.86 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.64 mg/mL (2.39 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.4 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.64 mg/mL (2.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.4 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7277 mL | 18.6386 mL | 37.2773 mL | |
| 5 mM | 0.7455 mL | 3.7277 mL | 7.4555 mL | |
| 10 mM | 0.3728 mL | 1.8639 mL | 3.7277 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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